{"created":"2021-03-01T06:10:47.092010+00:00","id":6939,"links":{},"metadata":{"_buckets":{"deposit":"ff89e970-89a6-432f-81e2-521813f8b299"},"_deposit":{"id":"6939","owners":[],"pid":{"revision_id":0,"type":"depid","value":"6939"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00006939","sets":["461:463:805:815"]},"author_link":["16840","16841","16842"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-02-28","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"8","bibliographicPageStart":"1","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"紀要"}]}]},"item_10_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We established a HPLC method for determination of α-Tocopherol (Vitamin E) in serum and liver tissues. α-Tocopherol was extracted from serum and liver homogenate with a mixture of n-hexane and ethanol (4 : 1 v/v). The extracts were dried under gas flow of nitrogen at 40℃, and the residus were reconstituted with 200㎕ of 96% methanol. Twenty㎕ of the reconstituted specimen was injected into HPLC system equiped with reverse phase ODS column 150×4.6mm i.d., 20㎕ loop injector and fluorospectrophotometric detector (Ex.286nm, Em. 325nm) , and eluted with 96 % methanol. Within run precision, mean±SD and CV (obtained with this method) were 15.1± 0.36㎍/㎖, 2.4% for serum (n=15) and 23.9±1.20㎍/㎖, 5.0 % for liver tissues (n=10).  The values of α-Tocopherol (mean±SD) in serum from healthy adults (n =20) and in liver tissues from mongrel dogs (n=-20) were 14.0±4.42㎍/㎖ and 20.7±7.41㎍/g , respectively. The method has an enough reproducibility and can be applied for determination of α-Tocopherol in biological materials in the field of laboratory medicine.","subitem_description_type":"Abstract"}]},"item_10_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_10_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"紀要 18: 1-8(1993)","subitem_description_type":"Other"}]},"item_10_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"信州大学医療技術短期大学部"}]},"item_10_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-1982","subitem_source_identifier_type":"ISSN"}]},"item_10_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10402438","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"寺澤,  文子","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"内川,  典子","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"野本,  昭三"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-25"}],"displaytype":"detail","filename":"Allied_Med18_01.pdf","filesize":[{"value":"371.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Allied_Med18_01.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/6939/files/Allied_Med18_01.pdf"},"version_id":"51247393-a171-41a7-b0cd-e64f01f6df53"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Vitamin E","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"α-Tocopherol","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HPLC","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fluorospectrophotometry","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"allowable error limit","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"高速液体クロマトグラフィーによる血清および肝組織中α-トコフェロール(ビタミンE)定量法の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"高速液体クロマトグラフィーによる血清および肝組織中α-トコフェロール(ビタミンE)定量法の検討","subitem_title_language":"ja"},{"subitem_title":"Studies on the HPLC Method for Determination of　α-Tocopherol (Vitamine E) in Serum and Liver Tissues","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["815"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-03-01"},"publish_date":"2010-03-01","publish_status":"0","recid":"6939","relation_version_is_last":true,"title":["高速液体クロマトグラフィーによる血清および肝組織中α-トコフェロール(ビタミンE)定量法の検討"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-02-13T01:06:23.136076+00:00"}