{"created":"2021-03-01T06:11:01.312062+00:00","id":7173,"links":{},"metadata":{"_buckets":{"deposit":"286634f2-236a-4d30-9f64-8c692bac7355"},"_deposit":{"id":"7173","owners":[],"pid":{"revision_id":0,"type":"depid","value":"7173"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00007173","sets":["461:848:849"]},"author_link":["17266","17267","17268","17269","17270","17271","17272","17273","17274","17275","17276"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"946","bibliographicPageStart":"939","bibliographicVolumeNumber":"106","bibliographic_titles":[{"bibliographic_title":"BRITISH JOURNAL OF CANCER"}]}]},"item_6_description_19":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"信州大学博士（医学）・学位論文・平成25年3月31日授与（甲第945号）・境澤 香里","subitem_description_type":"Other"}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND: The availability of molecular-targeted therapies for the treatment of melanoma has emphasised the need to identify mutations in target genes such as BRAF and KIT. Circulating tumour cells (CTC) are present in the peripheral blood of a significant proportion of cancer patients. METHODS: High molecular weight melanoma-associated antigen (HMW-MAA) was used to isolate melanoma cells from peripheral blood as it is selectively expressed at high levels on melanomas. The HMW-MAA-positive cells were isolated using immunomagnetic beads. After removing CD45(+) cells, CTC were identified by staining with MART-1-and gp100-specific antibodies (HMW-MAA(+), CD45(-), MART-1/gp100(+)). Single, isolated CTC were then subjected to BRAF and KIT mutational analysis. RESULTS: CTC (HMW-MAA(+), CD45(-), MART-1/gp100(+)) were isolated from the blood of 11 patients and BRAF and KIT were sequenced in nine and four patients, respectively. The BRAF sequences identified in the CTC were inconsistent with those identified in autologous melanoma tumours in three patients and the KIT sequences were inconsistent in three patients. In addition, polyclonal BRAF mutations were identified in one patient and concomitant mutations in BRAF and KIT were identified in another patient. CONCLUSION: Melanoma cells show clonal heterogeneity. Therefore, CTC genotyping may be crucial for successful molecular-targeted therapy. British Journal of Cancer (2012) 106, 939-946. doi:10.1038/bjc.2012.12 www.bjcancer.com Published online 26 January 2012 (C) 2012 Cancer Research UK","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"BRITISH JOURNAL OF CANCER. 106(5):939-946 (2012)","subitem_description_type":"Other"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"NATURE PUBLISHING GROUP"}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1038/bjc.2012.12"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/bjc.2012.12","subitem_relation_type_select":"DOI"}}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sakaizawa,  K."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Goto,  Y."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kiniwa,  Y."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uchiyama,  A."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Harada,  K."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimada,  S."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saida,  T."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ferrone,  S."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takata,  M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uhara,  H."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okuyama,  R."}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-25"}],"displaytype":"detail","filename":"H24Kou945_Sakaizawa.pdf","filesize":[{"value":"542.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"H24Kou945_Sakaizawa.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/7173/files/H24Kou945_Sakaizawa.pdf"},"version_id":"3480ade8-0e6d-440b-8a59-ca6036f69d07"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Mutation analysis of BRAF and KIT in circulating melanoma cells at the single cell level","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Mutation analysis of BRAF and KIT in circulating melanoma cells at the single cell level","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["849"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-06-27"},"publish_date":"2014-06-27","publish_status":"0","recid":"7173","relation_version_is_last":true,"title":["Mutation analysis of BRAF and KIT in circulating melanoma cells at the single cell level"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:31:56.053927+00:00"}