{"created":"2021-03-01T06:11:02.277059+00:00","id":7189,"links":{},"metadata":{"_buckets":{"deposit":"c428f87a-feec-486e-9017-942124757774"},"_deposit":{"id":"7189","owners":[],"pid":{"revision_id":0,"type":"depid","value":"7189"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00007189","sets":["461:848:849"]},"author_link":["17409","17410","17411","17412","17413","17414","17415","17416"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"607","bibliographicPageStart":"599","bibliographicVolumeNumber":"60","bibliographic_titles":[{"bibliographic_title":"ENDOCRINE JOURNAL"}]}]},"item_6_description_19":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"信州大学博士（医学）・学位論文・平成24年3月31日授与（甲第936号）・武井 真大","subitem_description_type":"Other"}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In pancreatic beta-cells, glucose metabolism leads to closure of ATP sensitive K+ channels (K-ATP channel) and Ca2+ influx, which is regarded as a required step for triggering of insulin release. Here, we demonstrate that glucose triggers rapid insulin release independent from its action on K-ATP channels given the cellular cAMP is elevated. We measured insulin release from rat pancreatic islets by static and perifusion experiments. Changes in cytosolic free Ca2+ concentration ([Ca2+](i)) were monitored using fura-2 loaded rat pancreatic beta-cells. Glucose-induced insulin release was abolished when Ca2+ influx was inhibited by a combination of 250 mu M diazoxide, an opener of K-ATP channel, and 10 mu M nifedipine, a blocker of L-type voltage-dependent Ca2+ channels. However, with both nifedipine and diazoxide, glucose induced a 5-fold increase in insulin release in the presence of 10 mu M forskolin, an activator of adenylyl cyclase. In the presence of diazoxide, nifedipine, and forskolin, 22 mM glucose sharply increased the rate of insulin release within 2 min which peaked at 6 min: this was followed by a further gradual increase in insulin release. In contrast, it lowered [Ca2+](i) with a nadir at 2-3 min followed by a gradual increase in [Ca2+](i). The glucose effect was mimicked by 20 mM alpha-ketoisocaproic acid, a mitochondrial fuel, and it was nullified by 2 mM sodium azide, an inhibitor of mitochondrial electron transport. Cerulenin, an inhibitor of protein acylation, decreased the glucose effect. In conclusion, a rise in [Ca2+](i) through voltage-dependent Ca2+ channels is not mandatory for glucose-induced triggering of insulin release.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"ENDOCRINE JOURNAL. 60(5):599-607 (2013)","subitem_description_type":"Other"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"JAPAN ENDOCRINE SOC"}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1507/endocrj.EJ12-0388"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1507/endocrj.EJ12-0388","subitem_relation_type_select":"DOI"}}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takei,  Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Dezaki,  Katsuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishii,  Hiroaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishio,  Shin-ichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato,  Yoshihiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki,  Satoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yada,  Toshihiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Komatsu,  Mitsuhisa"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-25"}],"displaytype":"detail","filename":"H24Kou936_Takei.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"H24Kou936_Takei.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/7189/files/H24Kou936_Takei.pdf"},"version_id":"628a0e0c-76b1-4424-8fa6-f92ba5049c3b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A new experimental model of ATP-sensitive K+ channel-independent insulinotropic action of glucose: a permissive role of cAMP for triggering of insulin release from rat pancreatic beta-cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A new experimental model of ATP-sensitive K+ channel-independent insulinotropic action of glucose: a permissive role of cAMP for triggering of insulin release from rat pancreatic beta-cells","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["849"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-06-27"},"publish_date":"2014-06-27","publish_status":"0","recid":"7189","relation_version_is_last":true,"title":["A new experimental model of ATP-sensitive K+ channel-independent insulinotropic action of glucose: a permissive role of cAMP for triggering of insulin release from rat pancreatic beta-cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T03:54:12.074358+00:00"}