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Traditional approaches for production of anti-AGE antibodies use cross-linkers such as 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) to conjugate the AGE antigen to the carrier protein. However, these approaches often fail to produce antibodies that are specific to the particular AGE of interest. In the present study, N-epsilon-(carboxymethyl)lysine (CML), a major antigenic AGE structure, was conjugated to human serum albumin (HSA) using various cross-linkers, including EDC, bis(sulfosuccinimidyl) suberate (BS3) and glutaraldehyde, to compare their efficiency for the production of epitope-specific antibodies. All of the cross-linkers tested were capable of conjugating CML to HSA, and each CML-conjugated HSA was recognized by previously characterized anti-CML antibody. However, only the use of glutaraldehyde as the cross-linker resulted in the production of a CML-specific monoclonal antibody, termed 2G11.2G11 significantly recognized CML-modified HSA and peptide, whereas it did not recognize N-epsilon-(carboxyethyl)lysine (CEL)-modified HSA and peptide, indicating that 2G11 is highly specific to CML, and can distinguish the difference of a single methyl group between the two epitopes. To further demonstrate the use of glutaraldehyde, anti-AGE antibodies against CEL, S-(2-succinyl) cysteine and S-(carboxymethyl)cysteine were obtained by conjugation with glutaraldehyde. These studies demonstrate the efficacy of glutaraldehyde as a cross-linker for the production of antibodies against small molecules. (C) 2008 Elsevier B.V. All rights reserved."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mera, K", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8371", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagai, M", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8372", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Brock, JWC", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8373", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujiwara, Y", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8374", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Murata, T", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8375", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Maruyama, T", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8376", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Baynes, JW", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8377", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Otagiri, M", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8378", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagai, R", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8379", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Merarevisedms.pdf", "filesize": [{"value": "84.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 84200.0, "url": {"label": "Merarevisedms.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/3976/files/Merarevisedms.pdf"}, "version_id": "f994e396-f6f6-47ce-b345-e96bbabcfcb3"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Glutaraldehyde is an effective cross-linker for production of antibodies against advanced glycation end-products", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Glutaraldehyde is an effective cross-linker for production of antibodies against advanced glycation end-products", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["462"], "permalink_uri": "http://hdl.handle.net/10091/1104", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-07-17"}, "publish_date": "2008-07-17", "publish_status": "0", "recid": "3976", "relation": {}, "relation_version_is_last": true, "title": ["Glutaraldehyde is an effective cross-linker for production of antibodies against advanced glycation end-products"], "weko_shared_id": -1}
Glutaraldehyde is an effective cross-linker for production of antibodies against advanced glycation end-products
http://hdl.handle.net/10091/1104
http://hdl.handle.net/10091/1104280456f0-fe2f-41fb-a86c-b72bbecb6f09
名前 / ファイル | ライセンス | アクション |
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Merarevisedms.pdf (84.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-07-17 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Glutaraldehyde is an effective cross-linker for production of antibodies against advanced glycation end-products | |||||
言語 | ||||||
言語 | eng | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.jim.2008.02.002 | |||||
関連名称 | 10.1016/j.jim.2008.02.002 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Mera, K
× Mera, K× Nagai, M× Brock, JWC× Fujiwara, Y× Murata, T× Maruyama, T× Baynes, JW× Otagiri, M× Nagai, R |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Murata, T | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.jpApONfU.html | |||||
出版者 | ||||||
出版者 | ELSEVIER SCIENCE BV | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | JOURNAL OF IMMUNOLOGICAL METHODS. 334(1-2): 82-90 (2008) | |||||
書誌情報 |
JOURNAL OF IMMUNOLOGICAL METHODS 巻 334, 号 1-2, p. 82-90, 発行日 2008-05-20 |
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資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0022-1759 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00699645 | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000255994500009 |