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Traditional approaches for production of anti-AGE antibodies use cross-linkers such as 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) to conjugate the AGE antigen to the carrier protein. However, these approaches often fail to produce antibodies that are specific to the particular AGE of interest. In the present study, N-epsilon-(carboxymethyl)lysine (CML), a major antigenic AGE structure, was conjugated to human serum albumin (HSA) using various cross-linkers, including EDC, bis(sulfosuccinimidyl) suberate (BS3) and glutaraldehyde, to compare their efficiency for the production of epitope-specific antibodies. All of the cross-linkers tested were capable of conjugating CML to HSA, and each CML-conjugated HSA was recognized by previously characterized anti-CML antibody. 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  1. 050 医学部, 大学院医学系研究科
  2. 0501 学術論文

Glutaraldehyde is an effective cross-linker for production of antibodies against advanced glycation end-products

http://hdl.handle.net/10091/1104
280456f0-fe2f-41fb-a86c-b72bbecb6f09
名前 / ファイル ライセンス アクション
Merarevisedms.pdf Merarevisedms.pdf (84.2 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-07-17
タイトル
言語 en
タイトル Glutaraldehyde is an effective cross-linker for production of antibodies against advanced glycation end-products
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Mera, K

× Mera, K

WEKO 8371

en Mera, K

Search repository
Nagai, M

× Nagai, M

WEKO 8372

en Nagai, M

Search repository
Brock, JWC

× Brock, JWC

WEKO 8373

en Brock, JWC

Search repository
Fujiwara, Y

× Fujiwara, Y

WEKO 8374

en Fujiwara, Y

Search repository
Murata, T

× Murata, T

WEKO 8375

en Murata, T

Search repository
Maruyama, T

× Maruyama, T

WEKO 8376

en Maruyama, T

Search repository
Baynes, JW

× Baynes, JW

WEKO 8377

en Baynes, JW

Search repository
Otagiri, M

× Otagiri, M

WEKO 8378

en Otagiri, M

Search repository
Nagai, R

× Nagai, R

WEKO 8379

en Nagai, R

Search repository
信州大学研究者総覧へのリンク
氏名 Murata, T
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.jpApONfU.html
出版者
出版者 ELSEVIER SCIENCE BV
引用
内容記述タイプ Other
内容記述 JOURNAL OF IMMUNOLOGICAL METHODS. 334(1-2): 82-90 (2008)
書誌情報 JOURNAL OF IMMUNOLOGICAL METHODS

巻 334, 号 1-2, p. 82-90, 発行日 2008-05-20
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ PISSN
収録物識別子 0022-1759
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00699645
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.jim.2008.02.002
関連名称
関連名称 10.1016/j.jim.2008.02.002
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000255994500009
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