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GLP-2-immunoreactive cells were observed as open-typed endocrine cells in the villous epithelium and crypts on both antigen retrieval agent-treated and untreated preparations. No obvious differences were detected in morphological features of GLP-2-immunoreactive cells between treated and untreated preparations. The frequencies of occurrence of GLP-2-immunoreactive cells, however, were significantly different in treated and untreated preparations: in the proximal and distal regions of jejunum and ileum obtained from untreated preparations, the frequencies of occurrence were 0.5 +/- 0.2, 0.7 +/- 0.1, 0.9 +/- 0.2 and 1.5 +/- 0.3, respectively (cell numbers per mucosal area: cells/mm(2), mean +/- SD), whereas those from treated sections were 14.7 +/- 2.3, 19.8 +/- 2.3, 23.5 +/- 4.7 and 34.6 +/- 4.9 cells/mm(2), respectively. These data indicate that this antigen retrieval method is able to make immunoreactive GLP-2 available for detection and that GLP-2 may act as one of the common hormones secreted by L cells in the chicken small intestine.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "JOURNAL OF VETERINARY MEDICAL SCIENCE. 76(4):565-568 (2014)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Hiramatsu, Kohzy", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.yeTmPpkh.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000335207800014"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "JAPAN SOC VET SCI"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "24334814"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/24334814/", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1292/jvms.13-0513"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1292/jvms.13-0513", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "Copyright\u00a9 2014 The Japanese Society of Veterinary Science"}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0916-7250", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0916-7250", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10796138", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "antigen retrieval agent; chicken; glucagon-like peptide-2; immunohistochemistry; intestine"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "GLP-2; PROGLUCAGON"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "An antigen retrieval method for immunohistochemical staining of glucagon-like peptide (GLP)-2-immunoreactive cells was investigated in the chicken small intestine. GLP-2-immunoreactive cells were observed as open-typed endocrine cells in the villous epithelium and crypts on both antigen retrieval agent-treated and untreated preparations. No obvious differences were detected in morphological features of GLP-2-immunoreactive cells between treated and untreated preparations. The frequencies of occurrence of GLP-2-immunoreactive cells, however, were significantly different in treated and untreated preparations: in the proximal and distal regions of jejunum and ileum obtained from untreated preparations, the frequencies of occurrence were 0.5 +/- 0.2, 0.7 +/- 0.1, 0.9 +/- 0.2 and 1.5 +/- 0.3, respectively (cell numbers per mucosal area: cells/mm(2), mean +/- SD), whereas those from treated sections were 14.7 +/- 2.3, 19.8 +/- 2.3, 23.5 +/- 4.7 and 34.6 +/- 4.9 cells/mm(2), respectively. These data indicate that this antigen retrieval method is able to make immunoreactive GLP-2 available for detection and that GLP-2 may act as one of the common hormones secreted by L cells in the chicken small intestine."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Monir,  Mohammad M.", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "32332", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hiramatsu,  Kohzy", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "32333", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishimura,  Kei", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "32334", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takemoto,  Chihiro", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "32335", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe,  Takafumi", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "32336", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Distribution_Glucagon-Like_Peptide_GLP-2-Immunoreactive_Cells.pdf", "filesize": [{"value": "2.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 2300000.0, "url": {"label": "Distribution_Glucagon-Like_Peptide_GLP-2-Immunoreactive_Cells.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/10242/files/Distribution_Glucagon-Like_Peptide_GLP-2-Immunoreactive_Cells.pdf"}, "version_id": "bda56929-1026-4fd7-ad15-6967ef603a2d"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "antigen retrieval agent", "subitem_subject_scheme": "Other"}, {"subitem_subject": "chicken", "subitem_subject_scheme": "Other"}, {"subitem_subject": "glucagon-like peptide-2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "immunohistochemistry", "subitem_subject_scheme": "Other"}, {"subitem_subject": "intestine", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Distribution of Glucagon-Like Peptide (GLP)-2-Immunoreactive Cells in the Chicken Small Intestine: Antigen Retrieval Immunohistochemistry", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Distribution of Glucagon-Like Peptide (GLP)-2-Immunoreactive Cells in the Chicken Small Intestine: Antigen Retrieval Immunohistochemistry", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1016/1017"], "permalink_uri": "http://hdl.handle.net/10091/17858", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2014-09-05"}, "publish_date": "2014-09-05", "publish_status": "0", "recid": "10242", "relation": {}, "relation_version_is_last": true, "title": ["Distribution of Glucagon-Like Peptide (GLP)-2-Immunoreactive Cells in the Chicken Small Intestine: Antigen Retrieval Immunohistochemistry"], "weko_shared_id": -1}