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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

Immunoelectron Microscopic Observation of Chicken Glucagon-Like Peptide (GLP)-1-Containing Cells in Tissues Derived from Thin Section, Paraffin Block and Conventional Method

http://hdl.handle.net/10091/17856
http://hdl.handle.net/10091/17856
7b4ba2e0-f890-4985-a4af-42647183080e
名前 / ファイル ライセンス アクション
Immunoelectron_Microscopic_Observation_Chicken_Glucagon-Like.pdf Immunoelectron_Microscopic_Observation_Chicken_Glucagon-Like.pdf (3.3 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-09-05
タイトル
言語 en
タイトル Immunoelectron Microscopic Observation of Chicken Glucagon-Like Peptide (GLP)-1-Containing Cells in Tissues Derived from Thin Section, Paraffin Block and Conventional Method
言語
言語 eng
キーワード
主題Scheme Other
主題 glucagon-like peptide-1
キーワード
主題Scheme Other
主題 immunocytochemistry
キーワード
主題Scheme Other
主題 paraffin-embedded tissue
キーワード
主題Scheme Other
主題 reprocessing method
キーワード
主題Scheme Other
主題 transmission electron microscope
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Watanabe, Takafumi

× Watanabe, Takafumi

WEKO 32347

ja Watanabe, Takafumi

Search repository
Nishimura, Kei

× Nishimura, Kei

WEKO 32348

ja Nishimura, Kei

Search repository
Monir, Mohammad M.

× Monir, Mohammad M.

WEKO 32349

ja Monir, Mohammad M.

Search repository
Takemoto, Chihiro

× Takemoto, Chihiro

WEKO 32350

ja Takemoto, Chihiro

Search repository
Hiramatsu, Kohzy

× Hiramatsu, Kohzy

WEKO 32351

ja Hiramatsu, Kohzy

Search repository
信州大学研究者総覧へのリンク
氏名 Hiramatsu, Kohzy
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.yeTmPpkh.html
出版者
出版者 JAPAN SOC VET SCI
引用
内容記述タイプ Other
内容記述 JOURNAL OF VETERINARY MEDICAL SCIENCE. 76(3):389-394 (2014)
書誌情報 JOURNAL OF VETERINARY MEDICAL SCIENCE

巻 76, 号 3, p. 389-394, 発行日 2014-03
抄録
内容記述タイプ Abstract
内容記述 The purpose of the present study was to investigate the possibility of immunoelectron microscopic observation of endocrine cells in paraffin-embedded tissues. The procedure, which involves reprocessing from sliced tissues and immunohistochemical staining by colloidal-gold immunolabeling of paraffin sections from paraffin blocks, was able to reveal the fine characteristics of secretory granules containing glucagon-like peptide-1. Morphometric analyses of the secretory granules showed no significant differences between the reprocessing procedure and a conventional post-embedding procedure, which was performed as a control. The reprocessing procedure has some advantages besides providing information on secretory granules containing the amino acid peptide. For example, the same cell can be observed under both a light microscope and the electron microscope. In addition, the high-electron densities of silver-enhanced gold particles are easily recognized, and the boundary between the profile of the granules and the immunogold labeling is clearly shown at the electron microscopic level. Furthermore, the procedure, which is inexpensive and does not require special devices, can effectively use precious samples that are already paraffin-embedded and unable to be obtained twice, such as the case for endangered animals and rare pathological tissues. To the best of our knowledge, the present study is the first to report the advantages of the reprocessing method for sliced paraffin sections of gut endocrine cells.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ PISSN
収録物識別子 0916-7250
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10796138
PubMed
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/24257328/
関連名称 24257328
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1292/jvms.13-0339
関連名称 10.1292/jvms.13-0339
権利
権利情報 Copyright© 2014 The Japanese Society of Veterinary Science
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000333777400010
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