{"_buckets": {"deposit": "8338a700-aefc-491f-9719-0dfc296eb38b"}, "_deposit": {"id": "10244", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "10244"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00010244", "sets": ["1017"]}, "author_link": ["32347", "32348", "32349", "32350", "32351"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "394", "bibliographicPageStart": "389", "bibliographicVolumeNumber": "76", "bibliographic_titles": [{"bibliographic_title": "JOURNAL OF VETERINARY MEDICAL SCIENCE"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The purpose of the present study was to investigate the possibility of immunoelectron microscopic observation of endocrine cells in paraffin-embedded tissues. 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The procedure, which involves reprocessing from sliced tissues and immunohistochemical staining by colloidal-gold immunolabeling of paraffin sections from paraffin blocks, was able to reveal the fine characteristics of secretory granules containing glucagon-like peptide-1. Morphometric analyses of the secretory granules showed no significant differences between the reprocessing procedure and a conventional post-embedding procedure, which was performed as a control. The reprocessing procedure has some advantages besides providing information on secretory granules containing the amino acid peptide. For example, the same cell can be observed under both a light microscope and the electron microscope. In addition, the high-electron densities of silver-enhanced gold particles are easily recognized, and the boundary between the profile of the granules and the immunogold labeling is clearly shown at the electron microscopic level. 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