ログイン
言語:

WEKO3

  • トップ
  • コミュニティ
  • ランキング
AND
To

Field does not validate



インデックスリンク

インデックスツリー

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "45bea227-0bf7-444a-9a7d-6cd6bed68143"}, "_deposit": {"id": "10255", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "10255"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00010255"}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "95", "bibliographicPageStart": "87", "bibliographicVolumeNumber": "51", "bibliographic_titles": [{"bibliographic_title": "JOURNAL OF POULTRY SCIENCE"}]}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Transplantation of primordial germ cells (PGCs), which are the progenitor cells of gametes, is a powerful tool for generation of transgenic chickens. However, the frequencies of transgene integration into the genome of purified PGCs still remain low. An in vitro culture system enabling chicken PGCs to propagate efficiently would be useful for efficient transgenesis of PGCs. In the present study, we optimized the culture conditions for chicken PGCs to enhance the proliferation and evaluated the germline transmission of cultured PGCs that proliferated for long periods of time. PGC-like cells (PGC-LCs), that have remarkably similar morphological characteristics to intact PGCs, could be derived by cultivation of blood containing PGCs obtained from 2.5-day-old chicken embryos according to the protocol of van de Lavoir et al. (2006). We determined which feeder cells and which growth factors were required to improve proliferation of PGC-LCs. Male PGC-LCs survival and proliferation were enhanced during culture in the basic medium containing either basic fibroblast growth factor (bFGF) alone or both bFGF and stem cell factor (SCF) on a feeder of buffalo rat liver (BRL) cells. Male PGC-LCs could be propagated in defined culture condition for extended periods. These cells expressed the germline-specific protein Vasa and undifferentiated cell marker stage-specific embryonic antigen-1 (SSEA-1) and pluripotency genes Nanog and PouV. Furthermore, Male PGC-LCs cultured for 225 d could migrate toward and colonize within recipient gonads and transmit to the next generation following transplantation. We succeeded in produce 3 offspring originating from long-term cultured PGC-LCs from a germline chimeric rooster (6%). The present study represents valuable steps toward defining a culture condition enabling PGC-LCs to propagate efficiently for long periods in vitro with maintenance of their commitment to the germline.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "JOURNAL OF POULTRY SCIENCE. 51(1):87-95 (2014)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Ono, Tamao", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html"}, {"subitem_link_text": "Kagami, Hiroshi", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000330202200013"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "JAPAN POULTRY SCIENCE ASSOC"}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.2141/jpsa.0130077"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.2141/jpsa.0130077", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "Copyright\u00a9 2014 Japan Poultry Science Association"}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1346-7395", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1346-7395", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11564513", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "chicken; germline chimera; PGCs; PGC-LCs"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "DENSITY GRADIENT CENTRIFUGATION; CHIMERIC CHICKENS; RECIPIENT EMBRYOS; GALLUS-DOMESTICUS; X-IRRADIATION; IN-VITRO; DIFFERENTIATION; PGCS; PURIFICATION; EXPRESSION"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Transplantation of primordial germ cells (PGCs), which are the progenitor cells of gametes, is a powerful tool for generation of transgenic chickens. However, the frequencies of transgene integration into the genome of purified PGCs still remain low. An in vitro culture system enabling chicken PGCs to propagate efficiently would be useful for efficient transgenesis of PGCs. In the present study, we optimized the culture conditions for chicken PGCs to enhance the proliferation and evaluated the germline transmission of cultured PGCs that proliferated for long periods of time. PGC-like cells (PGC-LCs), that have remarkably similar morphological characteristics to intact PGCs, could be derived by cultivation of blood containing PGCs obtained from 2.5-day-old chicken embryos according to the protocol of van de Lavoir et al. (2006). We determined which feeder cells and which growth factors were required to improve proliferation of PGC-LCs. Male PGC-LCs survival and proliferation were enhanced during culture in the basic medium containing either basic fibroblast growth factor (bFGF) alone or both bFGF and stem cell factor (SCF) on a feeder of buffalo rat liver (BRL) cells. Male PGC-LCs could be propagated in defined culture condition for extended periods. These cells expressed the germline-specific protein Vasa and undifferentiated cell marker stage-specific embryonic antigen-1 (SSEA-1) and pluripotency genes Nanog and PouV. Furthermore, Male PGC-LCs cultured for 225 d could migrate toward and colonize within recipient gonads and transmit to the next generation following transplantation. We succeeded in produce 3 offspring originating from long-term cultured PGC-LCs from a germline chimeric rooster (6%). The present study represents valuable steps toward defining a culture condition enabling PGC-LCs to propagate efficiently for long periods in vitro with maintenance of their commitment to the germline."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Miyahara,  Daichi"}], "nameIdentifiers": [{"nameIdentifier": "32400", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mori,  Takafumi"}], "nameIdentifiers": [{"nameIdentifier": "32401", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Makino,  Ryuichi"}], "nameIdentifiers": [{"nameIdentifier": "32402", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakamura,  Yoshiaki"}], "nameIdentifiers": [{"nameIdentifier": "32403", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oishi,  Isao"}], "nameIdentifiers": [{"nameIdentifier": "32404", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ono,  Tamao"}], "nameIdentifiers": [{"nameIdentifier": "32405", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nirasawa,  Keijiro"}], "nameIdentifiers": [{"nameIdentifier": "32406", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tagami,  Takahiro"}], "nameIdentifiers": [{"nameIdentifier": "32407", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kagami,  Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "32408", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-25"}], "displaytype": "detail", "download_preview_message": "", "filename": "Culture_Conditions_Maintain_Propagation.pdf", "filesize": [{"value": "1.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 16000000, "url": {"label": "Culture_Conditions_Maintain_Propagation.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/10255/files/Culture_Conditions_Maintain_Propagation.pdf"}, "version_id": "3efd3483-333c-47d4-98b3-f4bd93ce2e31"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "chicken", "subitem_subject_scheme": "Other"}, {"subitem_subject": "germline chimera", "subitem_subject_scheme": "Other"}, {"subitem_subject": "PGCs", "subitem_subject_scheme": "Other"}, {"subitem_subject": "PGC-LCs", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Culture Conditions for Maintain Propagation, Long-term Survival and Germline Transmission of Chicken Primordial Germ Cell-Like Cells", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Culture Conditions for Maintain Propagation, Long-term Survival and Germline Transmission of Chicken Primordial Germ Cell-Like Cells"}]}, "item_type_id": "6", "owner": "1", "path": ["1016/1017"], "permalink_uri": "http://hdl.handle.net/10091/17812", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2014-08-06"}, "publish_date": "2014-08-06", "publish_status": "0", "recid": "10255", "relation": {}, "relation_version_is_last": true, "title": ["Culture Conditions for Maintain Propagation, Long-term Survival and Germline Transmission of Chicken Primordial Germ Cell-Like Cells"], "weko_shared_id": null}
  1. 070 農学部, 大学院農学研究科 (Faculty&Graduate School of Agriculture)
  2. 0701 学術論文 (Journal Articles)

Culture Conditions for Maintain Propagation, Long-term Survival and Germline Transmission of Chicken Primordial Germ Cell-Like Cells

http://hdl.handle.net/10091/17812
834b7a25-1dfc-4cd5-b350-b0a190a1bb69
プレビュー
名前 / ファイル ライセンス Actions
Culture_Conditions_Maintain_Propagation.pdf Culture_Conditions_Maintain_Propagation.pdf (1.6 MB)
item type 学術雑誌論文 / Journal Article(1)
公開日 2014-08-06
タイトル
タイトル Culture Conditions for Maintain Propagation, Long-term Survival and Germline Transmission of Chicken Primordial Germ Cell-Like Cells
言語
言語 eng
キーワード
主題 chicken
キーワード
主題 germline chimera
キーワード
主題 PGCs
キーワード
主題 PGC-LCs
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Miyahara, Daichi

× Miyahara, Daichi

WEKO 32400

Miyahara, Daichi

Search repository
Mori, Takafumi

× Mori, Takafumi

WEKO 32401

Mori, Takafumi

Search repository
Makino, Ryuichi

× Makino, Ryuichi

WEKO 32402

Makino, Ryuichi

Search repository
Nakamura, Yoshiaki

× Nakamura, Yoshiaki

WEKO 32403

Nakamura, Yoshiaki

Search repository
Oishi, Isao

× Oishi, Isao

WEKO 32404

Oishi, Isao

Search repository
Ono, Tamao

× Ono, Tamao

WEKO 32405

Ono, Tamao

Search repository
Nirasawa, Keijiro

× Nirasawa, Keijiro

WEKO 32406

Nirasawa, Keijiro

Search repository
Tagami, Takahiro

× Tagami, Takahiro

WEKO 32407

Tagami, Takahiro

Search repository
Kagami, Hiroshi

× Kagami, Hiroshi

WEKO 32408

Kagami, Hiroshi

Search repository
信州大学研究者総覧へのリンク
Ono, Tamao
http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html
信州大学研究者総覧へのリンク
Kagami, Hiroshi
http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html
出版者
出版者 JAPAN POULTRY SCIENCE ASSOC
引用
内容記述タイプ Other
内容記述 JOURNAL OF POULTRY SCIENCE. 51(1):87-95 (2014)
書誌情報 JOURNAL OF POULTRY SCIENCE

巻 51, 号 1, p. 87-95, 発行日 2014-01
抄録
内容記述タイプ Abstract
内容記述 Transplantation of primordial germ cells (PGCs), which are the progenitor cells of gametes, is a powerful tool for generation of transgenic chickens. However, the frequencies of transgene integration into the genome of purified PGCs still remain low. An in vitro culture system enabling chicken PGCs to propagate efficiently would be useful for efficient transgenesis of PGCs. In the present study, we optimized the culture conditions for chicken PGCs to enhance the proliferation and evaluated the germline transmission of cultured PGCs that proliferated for long periods of time. PGC-like cells (PGC-LCs), that have remarkably similar morphological characteristics to intact PGCs, could be derived by cultivation of blood containing PGCs obtained from 2.5-day-old chicken embryos according to the protocol of van de Lavoir et al. (2006). We determined which feeder cells and which growth factors were required to improve proliferation of PGC-LCs. Male PGC-LCs survival and proliferation were enhanced during culture in the basic medium containing either basic fibroblast growth factor (bFGF) alone or both bFGF and stem cell factor (SCF) on a feeder of buffalo rat liver (BRL) cells. Male PGC-LCs could be propagated in defined culture condition for extended periods. These cells expressed the germline-specific protein Vasa and undifferentiated cell marker stage-specific embryonic antigen-1 (SSEA-1) and pluripotency genes Nanog and PouV. Furthermore, Male PGC-LCs cultured for 225 d could migrate toward and colonize within recipient gonads and transmit to the next generation following transplantation. We succeeded in produce 3 offspring originating from long-term cultured PGC-LCs from a germline chimeric rooster (6%). The present study represents valuable steps toward defining a culture condition enabling PGC-LCs to propagate efficiently for long periods in vitro with maintenance of their commitment to the germline.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 1346-7395
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11564513
DOI
関連識別子
識別子タイプ DOI
関連識別子 http://dx.doi.org/10.2141/jpsa.0130077
関連名称
関連名称 10.2141/jpsa.0130077
権利
権利情報 Copyright© 2014 Japan Poultry Science Association
著者版フラグ
値 publisher
WoS
Web of Science
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000330202200013
Back
0
views
See details
Views

Versions

Ver.1 2021-03-01 12:16:56.812686
Show All versions

Share

Mendeley CiteULike Twitter Facebook Print Addthis

Cite as

Export

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by CERN Data Centre & Invenio


Powered by CERN Data Centre & Invenio