| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2014-08-06 |
| タイトル |
|
|
タイトル |
Culture Conditions for Maintain Propagation, Long-term Survival and Germline Transmission of Chicken Primordial Germ Cell-Like Cells |
| 言語 |
|
|
言語 |
eng |
| DOI |
|
|
|
関連識別子 |
https://doi.org/10.2141/jpsa.0130077 |
|
|
関連名称 |
10.2141/jpsa.0130077 |
| キーワード |
|
|
主題 |
chicken, germline chimera, PGCs, PGC-LCs |
| 資源タイプ |
|
|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Miyahara, Daichi
Mori, Takafumi
Makino, Ryuichi
Nakamura, Yoshiaki
Oishi, Isao
Ono, Tamao
Nirasawa, Keijiro
Tagami, Takahiro
Kagami, Hiroshi
|
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Ono, Tamao |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Kagami, Hiroshi |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html |
| 出版者 |
|
|
出版者 |
JAPAN POULTRY SCIENCE ASSOC |
| 引用 |
|
|
内容記述 |
JOURNAL OF POULTRY SCIENCE. 51(1):87-95 (2014) |
| 書誌情報 |
JOURNAL OF POULTRY SCIENCE
巻 51,
号 1,
p. 87-95,
発行日 2014-01
|
| 抄録 |
|
|
内容記述 |
Transplantation of primordial germ cells (PGCs), which are the progenitor cells of gametes, is a powerful tool for generation of transgenic chickens. However, the frequencies of transgene integration into the genome of purified PGCs still remain low. An in vitro culture system enabling chicken PGCs to propagate efficiently would be useful for efficient transgenesis of PGCs. In the present study, we optimized the culture conditions for chicken PGCs to enhance the proliferation and evaluated the germline transmission of cultured PGCs that proliferated for long periods of time. PGC-like cells (PGC-LCs), that have remarkably similar morphological characteristics to intact PGCs, could be derived by cultivation of blood containing PGCs obtained from 2.5-day-old chicken embryos according to the protocol of van de Lavoir et al. (2006). We determined which feeder cells and which growth factors were required to improve proliferation of PGC-LCs. Male PGC-LCs survival and proliferation were enhanced during culture in the basic medium containing either basic fibroblast growth factor (bFGF) alone or both bFGF and stem cell factor (SCF) on a feeder of buffalo rat liver (BRL) cells. Male PGC-LCs could be propagated in defined culture condition for extended periods. These cells expressed the germline-specific protein Vasa and undifferentiated cell marker stage-specific embryonic antigen-1 (SSEA-1) and pluripotency genes Nanog and PouV. Furthermore, Male PGC-LCs cultured for 225 d could migrate toward and colonize within recipient gonads and transmit to the next generation following transplantation. We succeeded in produce 3 offspring originating from long-term cultured PGC-LCs from a germline chimeric rooster (6%). The present study represents valuable steps toward defining a culture condition enabling PGC-LCs to propagate efficiently for long periods in vitro with maintenance of their commitment to the germline. |
| 資源タイプ（コンテンツの種類） |
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1346-7395 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11564513 |
| 権利 |
|
|
権利情報 |
Copyright© 2014 Japan Poultry Science Association |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| WoS |
|
|
URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000330202200013 |
Culture_Conditions_Maintain_Propagation.pdf (1.6 MB)
