| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2014-08-06 |
| タイトル |
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|
タイトル |
Development of Fatty Acid-Producing Corynebacterium glutamicum Strains |
| 言語 |
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|
言語 |
eng |
| DOI |
|
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|
関連識別子 |
https://doi.org/10.1128/AEM.02003-13 |
|
|
関連名称 |
10.1128/AEM.02003-13 |
| 資源タイプ |
|
|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Takeno, Seiki
Takasaki, Manami
Urabayashi, Akinobu
Mimura, Akinori
Muramatsu, Tetsuhiro
Mitsuhashi, Satoshi
Ikeda, Masato
|
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Takeno, Seiki |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Ikeda, Masato |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html |
| 出版者 |
|
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出版者 |
AMER SOC MICROBIOLOGY |
| 引用 |
|
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内容記述 |
APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 79(21):6776-6783 (2013) |
| 書誌情報 |
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
巻 79,
号 21,
p. 6776-6783,
発行日 2013-11
|
| 抄録 |
|
|
内容記述 |
To date, no information has been made available on the genetic traits that lead to increased carbon flow into the fatty acid biosynthetic pathway of Corynebacterium glutamicum. To develop basic technologies for engineering, we employed an approach that begins by isolating a fatty acid-secreting mutant without depending on mutagenic treatment. This was followed by genome analysis to characterize its genetic background. The selection of spontaneous mutants resistant to the palmitic acid ester surfactant Tween 40 resulted in the isolation of a desired mutant that produced oleic acid, suggesting that a single mutation would cause increased carbon flow down the pathway and subsequent excretion of the oversupplied fatty acid into the medium. Two additional rounds of selection of spontaneous cerulenin-resistant mutants led to increased production of the fatty acid in a stepwise manner. Whole-genome sequencing of the resulting best strain identified three specific mutations (fasR20, fasA63(up), and fasA2623). Allele-specific PCR analysis showed that the mutations arose in that order. Reconstitution experiments with these mutations revealed that only fasR20 gave rise to oleic acid production in the wild-type strain. The other two mutations contributed to an increase in oleic acid production. Deletion of fasR from the wild-type strain led to oleic acid production as well. Reverse transcription-quantitative PCR analysis revealed that the fasR20 mutation brought about upregulation of the fasA and fasB genes encoding fatty acid synthases IA and IB, respectively, by 1.31-fold +/- 0.11-fold and 1.29-fold +/- 0.12-fold, respectively, and of the accD1 gene encoding the beta-subunit of acetyl-CoA carboxylase by 3.56-fold +/- 0.97-fold. On the other hand, the fasA63(up) mutation upregulated the fasA gene by 2.67-fold +/- 0.16-fold. In flask cultivation with 1% glucose, the fasR20 fasA63(up) fasA2623 triple mutant produced approximately 280 mg of fatty acids/liter, which consisted mainly of oleic acid (208 mg/liter) and palmitic acid (47 mg/liter). |
| 資源タイプ（コンテンツの種類） |
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| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0099-2240 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00543249 |
| PubMed |
|
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/23995924/ |
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|
関連名称 |
23995924 |
| 権利 |
|
|
権利情報 |
Copyright© 2013 American Society for Microbiology. |
| 出版タイプ |
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|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| WoS |
|
|
URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000326193300029 |
Development_Fatty_Acid-Producing_Corynebacterium_glutamicum.pdf (769.5 kB)
