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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

Expression of a Biologically Active GFP-alpha(S1)-Casein Fusion Protein in Lactococcus lactis

http://hdl.handle.net/10091/16133
http://hdl.handle.net/10091/16133
08cf4243-77cd-4b93-b273-1887b5b943e7
名前 / ファイル ライセンス アクション
Expression_Biologically_Active_GFP-alphaS1-Casein_Fusion.pdf Expression_Biologically_Active_GFP-alphaS1-Casein_Fusion.pdf (474.1 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2012-12-10
タイトル
言語 en
タイトル Expression of a Biologically Active GFP-alpha(S1)-Casein Fusion Protein in Lactococcus lactis
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Shigemori, Suguru

× Shigemori, Suguru

WEKO 32565

Shigemori, Suguru

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Yonekura, Shinichi

× Yonekura, Shinichi

WEKO 32566

Yonekura, Shinichi

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Sato, Takashi

× Sato, Takashi

WEKO 32567

Sato, Takashi

Search repository
Nakanishi, Maya

× Nakanishi, Maya

WEKO 32568

Nakanishi, Maya

Search repository
Otani, Hajime

× Otani, Hajime

WEKO 32569

Otani, Hajime

Search repository
Shimosato, Takeshi

× Shimosato, Takeshi

WEKO 32570

Shimosato, Takeshi

Search repository
信州大学研究者総覧へのリンク
氏名 Yonekura, Shinichi
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.gakFPUkh.html
信州大学研究者総覧へのリンク
氏名 Shimosato, Takeshi
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html
出版者
出版者 SPRINGER
引用
内容記述タイプ Other
内容記述 CURRENT MICROBIOLOGY. 64(6):569-575 (2012)
書誌情報 CURRENT MICROBIOLOGY

巻 64, 号 6, p. 569-575, 発行日 2012-06
抄録
内容記述タイプ Abstract
内容記述 In this study, we successfully developed a recombinant strain of Lactococcus lactis NZ9000 (NZ9000) that produced green fluorescent protein fused to alpha(S1)-casein (GFP-alpha(S1)Cas). A modified lactic acid bacterial vector (pNZ8148#2) was constructed by inserting genes for GFP and alpha(S1)-casein, a major cow's milk allergen, and the resulting vector, pNZ8148#2-GFP-alpha(S1)Cas, was applied to the expression of recombinant GFP-alpha(S1)Cas protein (rGFP-alpha(S1)Cas) in NZ9000. After inducing expression with nisin, the production of rGFP-alpha(S1)Cas was confirmed by confocal laser microscopic analysis, and the expression conditions were optimized based on fluorescent analysis and western blotting results. Moreover, the in vitro treatment of splenocytes isolated from alpha-casein (a parts per thousand yen70 % alpha(S)-casein)-immunized mice with rGFP-alpha(S1)Cas resulted in increased IL-13 mRNA expression. The observed allergic activity is indicative of the Th2-cell mediated immune response and is similar to the effects induced by exposure to alpha-casein. Our results suggest that the expression of rGFP-alpha(S1)Cas in NZ9000 may facilitate in vivo applications of this system aimed at improving the specificity of immunological responses to specific milk allergen.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0343-8651
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00621783
PubMed
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/22437853/
関連名称 22437853
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1007/s00284-012-0111-x
関連名称 10.1007/s00284-012-0111-x
権利
権利情報 The original publication is available at www.springerlink.com
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000303402000008
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