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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

Identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in Corynebacterium glutamicum

http://hdl.handle.net/10091/16130
80bc8e79-9c28-4834-8c96-f0cb90f4c882
名前 / ファイル ライセンス アクション
Identification_application_different_glucose_uptake_system.pdf Identification_application_different_glucose_uptake_system.pdf (255.6 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2012-12-06
タイトル
言語 en
タイトル Identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in Corynebacterium glutamicum
言語
言語 eng
キーワード
主題Scheme Other
主題 Glucose uptake bypass
キーワード
主題Scheme Other
主題 Phosphotransferase system
キーワード
主題Scheme Other
主題 myo-inositol transporter
キーワード
主題Scheme Other
主題 Corynebacterium glutamicum
キーワード
主題Scheme Other
主題 Lysine production
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Ikeda, Masato

× Ikeda, Masato

WEKO 32576

Ikeda, Masato

Search repository
Mizuno, Yuta

× Mizuno, Yuta

WEKO 32577

Mizuno, Yuta

Search repository
Awane, Shin-ichi

× Awane, Shin-ichi

WEKO 32578

Awane, Shin-ichi

Search repository
Hayashi, Masahiro

× Hayashi, Masahiro

WEKO 32579

Hayashi, Masahiro

Search repository
Mitsuhashi, Satoshi

× Mitsuhashi, Satoshi

WEKO 32580

Mitsuhashi, Satoshi

Search repository
Takeno, Seiki

× Takeno, Seiki

WEKO 32581

Takeno, Seiki

Search repository
信州大学研究者総覧へのリンク
氏名 Ikeda, Masato
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html
信州大学研究者総覧へのリンク
氏名 Takeno, Seiki
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html
出版者
出版者 SPRINGER
引用
内容記述タイプ Other
内容記述 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 90(4):1443-1451 (2011)
書誌情報 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

巻 90, 号 4, p. 1443-1451, 発行日 2011-05
抄録
内容記述タイプ Abstract
内容記述 Corynebacterium glutamicum uses the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) to uptake and phosphorylate glucose; no other route has yet been identified. Disruption of the ptsH gene in wild-type C. glutamicum resulted, as expected, in a phenotype exhibiting little growth on any of the PTS sugars: glucose, fructose, and sucrose. However, a suppressor mutant that grew on glucose but not on the other two sugars was spontaneously isolated from the PTS-negative strain WT Delta ptsH. The suppressor strain SPH2, unlike the wild-type strain, exhibited a phenotype of resistance to 2-deoxyglucose which is known to be a toxic substrate for the glucose-PTS of this microbe, suggesting that strain SPH2 utilizes glucose via a different system involving a permease and native glucokinases. Analysis of the C. glutamicum genome sequence using Escherichia coli galactose permease, which can transport glucose, led to the identification of two candidate genes, iolT1 and iolT2, both of which have been reported as myo-inositol transporters. When cultured on glucose medium supplemented with myo-inositol, strain WT Delta ptsH was able to consume glucose, suggesting that glucose uptake was mediated by one or more myo-inositol-induced transporters. Overexpression of iolT1 alone and that of iolT2 alone under the gapA promoter in strain WT Delta ptsH rendered the strain capable of growing on glucose, proving that each transporter played a role in glucose uptake. Disruption of iolT1 in strain SPH2 abolished growth on glucose, whereas disruption of iolT2 did not, revealing that iolT1 was responsible for glucose uptake in strain SPH2. Sequence analysis of the iol gene cluster and its surrounding region identified a single-base deletion in the putative transcriptional regulator gene Cgl0157 of strain SPH2. Introduction of the frameshift mutation allowed strain WT Delta ptsH to grow on glucose, and further deletion of iolT1 abolished the growth again, indicating that inactivation of Cgl0157 under a PTS-negative background can be a means by which to express the iolT1-specified glucose uptake bypass instead of the native PTS. When this strategy was applied to a defined lysine producer, the engineered strain displayed increased lysine production from glucose.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0175-7598
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10503386
PubMed
関連識別子
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=21452034
関連名称
関連名称 21452034
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1007/s00253-011-3210-x
関連名称
関連名称 10.1007/s00253-011-3210-x
権利
権利情報 The original publication is available at www.springerlink.com
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000290574700024
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