| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2012-12-06 |
| タイトル |
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|
タイトル |
Identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in Corynebacterium glutamicum |
| 言語 |
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言語 |
eng |
| DOI |
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関連識別子 |
https://doi.org/10.1007/s00253-011-3210-x |
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関連名称 |
10.1007/s00253-011-3210-x |
| キーワード |
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主題 |
Glucose uptake bypass, Phosphotransferase system, myo-inositol transporter, Corynebacterium glutamicum, Lysine production |
| 資源タイプ |
|
|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Ikeda, Masato
Mizuno, Yuta
Awane, Shin-ichi
Hayashi, Masahiro
Mitsuhashi, Satoshi
Takeno, Seiki
|
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Ikeda, Masato |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Takeno, Seiki |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html |
| 出版者 |
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出版者 |
SPRINGER |
| 引用 |
|
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内容記述 |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 90(4):1443-1451 (2011) |
| 書誌情報 |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
巻 90,
号 4,
p. 1443-1451,
発行日 2011-05
|
| 抄録 |
|
|
内容記述 |
Corynebacterium glutamicum uses the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) to uptake and phosphorylate glucose; no other route has yet been identified. Disruption of the ptsH gene in wild-type C. glutamicum resulted, as expected, in a phenotype exhibiting little growth on any of the PTS sugars: glucose, fructose, and sucrose. However, a suppressor mutant that grew on glucose but not on the other two sugars was spontaneously isolated from the PTS-negative strain WT Delta ptsH. The suppressor strain SPH2, unlike the wild-type strain, exhibited a phenotype of resistance to 2-deoxyglucose which is known to be a toxic substrate for the glucose-PTS of this microbe, suggesting that strain SPH2 utilizes glucose via a different system involving a permease and native glucokinases. Analysis of the C. glutamicum genome sequence using Escherichia coli galactose permease, which can transport glucose, led to the identification of two candidate genes, iolT1 and iolT2, both of which have been reported as myo-inositol transporters. When cultured on glucose medium supplemented with myo-inositol, strain WT Delta ptsH was able to consume glucose, suggesting that glucose uptake was mediated by one or more myo-inositol-induced transporters. Overexpression of iolT1 alone and that of iolT2 alone under the gapA promoter in strain WT Delta ptsH rendered the strain capable of growing on glucose, proving that each transporter played a role in glucose uptake. Disruption of iolT1 in strain SPH2 abolished growth on glucose, whereas disruption of iolT2 did not, revealing that iolT1 was responsible for glucose uptake in strain SPH2. Sequence analysis of the iol gene cluster and its surrounding region identified a single-base deletion in the putative transcriptional regulator gene Cgl0157 of strain SPH2. Introduction of the frameshift mutation allowed strain WT Delta ptsH to grow on glucose, and further deletion of iolT1 abolished the growth again, indicating that inactivation of Cgl0157 under a PTS-negative background can be a means by which to express the iolT1-specified glucose uptake bypass instead of the native PTS. When this strategy was applied to a defined lysine producer, the engineered strain displayed increased lysine production from glucose. |
| 資源タイプ（コンテンツの種類） |
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0175-7598 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10503386 |
| PubMed |
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|
識別子タイプ |
PMID |
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関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/21452034/ |
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|
関連名称 |
21452034 |
| 権利 |
|
|
権利情報 |
The original publication is available at www.springerlink.com |
| 出版タイプ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| WoS |
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|
URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000290574700024 |
Identification_application_different_glucose_uptake_system.pdf (255.6 kB)
