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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

Engineering of Corynebacterium glutamicum with an NADPH-Generating Glycolytic Pathway for L-Lysine Production

http://hdl.handle.net/10091/13188
3f595403-e81c-4a90-b0dc-bde2745d3234
名前 / ファイル ライセンス アクション
Engineering_Corynebacterium_glutamicum.pdf Engineering_Corynebacterium_glutamicum.pdf (497.3 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2011-06-20
タイトル
言語 en
タイトル Engineering of Corynebacterium glutamicum with an NADPH-Generating Glycolytic Pathway for L-Lysine Production
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Takeno, Seiki

× Takeno, Seiki

WEKO 32624

Takeno, Seiki

Search repository
Murata, Ryosuke

× Murata, Ryosuke

WEKO 32625

Murata, Ryosuke

Search repository
Kobayashi, Ryosuke

× Kobayashi, Ryosuke

WEKO 32626

Kobayashi, Ryosuke

Search repository
Mitsuhashi, Satoshi

× Mitsuhashi, Satoshi

WEKO 32627

Mitsuhashi, Satoshi

Search repository
Ikeda, Masato

× Ikeda, Masato

WEKO 32628

Ikeda, Masato

Search repository
信州大学研究者総覧へのリンク
氏名 Takeno, Seiki
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html
信州大学研究者総覧へのリンク
氏名 Ikeda, Masato
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html
出版者
出版者 AMER SOC MICROBIOLOGY
引用
内容記述タイプ Other
内容記述 APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 76(21): 7154-7160(2010)
書誌情報 APPLIED AND ENVIRONMENTAL MICROBIOLOGY

巻 76, 号 21, p. 7154-7160, 発行日 2010-11
抄録
内容記述タイプ Abstract
内容記述 A sufficient supply of NADPH is a critical factor in L-lysine production by Corynebacterium glutamicum. Endogenous NAD-dependent glyceraldehyde 3-phosphate dehydrogenase (GAPDH) of C. glutamicum was replaced with nonphosphorylating NADP-dependent glyceraldehyde 3-phosphate dehydrogenase (GapN) of Streptococcus mutans, which catalyzes the reaction of glyceraldehyde 3-phosphate to 3-phosphoglycerate with the reduction of NADP(+) to NADPH, resulting in the reconstruction of the functional glycolytic pathway. Although the growth of the engineered strain on glucose was significantly retarded, a suppressor mutant with an increased ability to utilize sugars was spontaneously isolated from the engineered strain. The suppressor mutant was characterized by the properties of GapN as well as the nucleotide sequence of the gene, confirming that no change occurred in either the activity or the basic properties of GapN. The suppressor mutant was engineered into an L-lysine-producing strain by plasmid-mediated expression of the desensitized lysC gene, and the performance of the mutant as an L-lysine producer was evaluated. The amounts of L-lysine produced by the suppressor mutant were larger than those produced by the reference strain (which was created by replacement of the preexisting gapN gene in the suppressor mutant with the original gapA gene) by similar to 70% on glucose, similar to 120% on fructose, and similar to 100% on sucrose, indicating that the increased L-lysine production was attributed to GapN. These results demonstrate effective L-lysine production by C. glutamicum with an additional source of NADPH during glycolysis.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0099-2240
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00543249
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1128/AEM.01464-10
関連名称
関連名称 10.1128/AEM.01464-10
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000283439800022
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