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Engineering of Corynebacterium glutamicum with an NADPH-Generating Glycolytic Pathway for L-Lysine Production
http://hdl.handle.net/10091/13188
http://hdl.handle.net/10091/131883f595403-e81c-4a90-b0dc-bde2745d3234
| 名前 / ファイル | ライセンス | アクション |
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Engineering_Corynebacterium_glutamicum.pdf (497.3 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2011-06-20 | |||||
| タイトル | ||||||
| タイトル | Engineering of Corynebacterium glutamicum with an NADPH-Generating Glycolytic Pathway for L-Lysine Production | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| DOI | ||||||
| 関連識別子 | https://doi.org/10.1128/AEM.01464-10 | |||||
| 関連名称 | 10.1128/AEM.01464-10 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Takeno, Seiki
× Takeno, Seiki× Murata, Ryosuke× Kobayashi, Ryosuke× Mitsuhashi, Satoshi× Ikeda, Masato |
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| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Takeno, Seiki | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html | |||||
| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Ikeda, Masato | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html | |||||
| 出版者 | ||||||
| 出版者 | AMER SOC MICROBIOLOGY | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 76(21): 7154-7160(2010) | |||||
| 書誌情報 |
APPLIED AND ENVIRONMENTAL MICROBIOLOGY 巻 76, 号 21, p. 7154-7160, 発行日 2010-11 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A sufficient supply of NADPH is a critical factor in L-lysine production by Corynebacterium glutamicum. Endogenous NAD-dependent glyceraldehyde 3-phosphate dehydrogenase (GAPDH) of C. glutamicum was replaced with nonphosphorylating NADP-dependent glyceraldehyde 3-phosphate dehydrogenase (GapN) of Streptococcus mutans, which catalyzes the reaction of glyceraldehyde 3-phosphate to 3-phosphoglycerate with the reduction of NADP(+) to NADPH, resulting in the reconstruction of the functional glycolytic pathway. Although the growth of the engineered strain on glucose was significantly retarded, a suppressor mutant with an increased ability to utilize sugars was spontaneously isolated from the engineered strain. The suppressor mutant was characterized by the properties of GapN as well as the nucleotide sequence of the gene, confirming that no change occurred in either the activity or the basic properties of GapN. The suppressor mutant was engineered into an L-lysine-producing strain by plasmid-mediated expression of the desensitized lysC gene, and the performance of the mutant as an L-lysine producer was evaluated. The amounts of L-lysine produced by the suppressor mutant were larger than those produced by the reference strain (which was created by replacement of the preexisting gapN gene in the suppressor mutant with the original gapA gene) by similar to 70% on glucose, similar to 120% on fructose, and similar to 100% on sucrose, indicating that the increased L-lysine production was attributed to GapN. These results demonstrate effective L-lysine production by C. glutamicum with an additional source of NADPH during glycolysis. | |||||
| 資源タイプ（コンテンツの種類） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0099-2240 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00543249 | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| WoS | ||||||
| 表示名 | Web of Science | |||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000283439800022 | |||||
