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Restriction of Germline Proliferation by Soft X-ray Irradiation of Chicken Embryos and its Application to Chimera Production
http://hdl.handle.net/10091/13186
http://hdl.handle.net/10091/13186f88a60b7-cf31-4248-b702-358e9aaa33cd
| 名前 / ファイル | ライセンス | アクション |
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Restriction_Germline_Proliferation_Soft_X-ray_Irradiation.pdf (418.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2011-06-20 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Restriction of Germline Proliferation by Soft X-ray Irradiation of Chicken Embryos and its Application to Chimera Production | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| DOI | ||||||||||||||
| 関連識別子 | https://doi.org/10.2141/jpsa.45.292 | |||||||||||||
| 関連名称 | 10.2141/jpsa.45.292 | |||||||||||||
| キーワード | ||||||||||||||
| 主題 | blastoderm, chimera, embryo culture, primordial germ cells, soft x-ray | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| タイプ | journal article | |||||||||||||
| 著者 |
Atsumi, Yusuke
× Atsumi, Yusuke
× Tagami, Takahiro
× Kagami, Hiroshi
× Ono, Tamao
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| 信州大学研究者総覧へのリンク | ||||||||||||||
| 氏名 | Kagami, Hiroshi | |||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html | |||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||
| 氏名 | Ono, Tamao | |||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html | |||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | JAPAN POULTRY SCIENCE ASSOC | |||||||||||||
| 引用 | ||||||||||||||
| 内容記述 | JOURNAL OF POULTRY SCIENCE. 45(4): 292-297(2008) | |||||||||||||
| 書誌情報 |
JOURNAL OF POULTRY SCIENCE 巻 45, 号 4, p. 292-297, 発行日 2008-10-25 |
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| 抄録 | ||||||||||||||
| 内容記述 | Primordial germ cells (PGCs) are the progenitor cells of gametes. Avian PGCs are located in the central region of the area pellucida at the blastoderm stage. PGCs enter the circulation soon after the formation of blood vessels in incubating eggs and eventually settle in the gonadal primordium. We have now examined exposure of chicken embryos to soft (low-energy) x-rays as a means of depleting endogenous PGCs and thereby improving the efficiency of chimera production. The blastoderm of White Leghorn eggs was exposed to soft x-rays for 0, 20, 40 or 60s before incubation. The irradiated embryos manifested delayed development at 60 h of incubation. They also showed reduced numbers of circulating PGCs at stages 14 and 15 and of gonadal PGCs at stage 30. The hatchability of irradiated embryos was lower than that of nonirradiated controls. Irradiation for 20 s was found to provide the best outcome taking into consideration both the restriction of PGC proliferation and hatchability. Dispersed blastoderm cells of quail (black plumage) embryos were introduced into the blastoderm of chicken embryos irradiated for 20 s or of nonirradiated embryos. The number of donor-derived PGCs was higher in the irradiated embryos than in the nonirradiated controls at stage 30. These results suggest that soft x-irradiation of chicken embryos is a feasible approach to depletion of endogenous germ cells and consequent improvement in the efficiency of incorporation of donor PGCs. | |||||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 1346-7395 | |||||||||||||
| 書誌レコードID | ||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AA11564513 | |||||||||||||
| 異版である | ||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | URI | |||||||||||||
| 関連識別子 | http://joi.jlc.jst.go.jp/JST.JSTAGE/jpsa/45.292 | |||||||||||||
| 権利 | ||||||||||||||
| 権利情報 | Copyright (c) 2008 by Japan Poultry Science Association | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| WoS | ||||||||||||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000260362900009 | |||||||||||||
