| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2011-06-20 |
| タイトル |
|
|
タイトル |
A Novel Concentrating System of Chicken Stem Cells by Bone Marrow Side Population Cells |
| 言語 |
|
|
言語 |
eng |
| DOI |
|
|
|
関連識別子 |
https://doi.org/10.2141/jpsa.009052 |
|
|
関連名称 |
10.2141/jpsa.009052 |
| キーワード |
|
|
主題 |
bone marrow cells, chicken, flow cytometric analysis, hematopoietic stem cells, side population |
| 資源タイプ |
|
|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Usui, Fumitake
Nakamura, Yoshiaki
Yamamoto, Yasuhiro
Tatsumi, Kouichi
Tominari, Kazue
Ono, Tamao
Kagami, Hiroshi
|
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Ono, Tamao |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Kagami, Hiroshi |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html |
| 出版者 |
|
|
出版者 |
JAPAN POULTRY SCIENCE ASSOC |
| 引用 |
|
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内容記述 |
JOURNAL OF POULTRY SCIENCE. 47(1): 53-56(2010) |
| 書誌情報 |
JOURNAL OF POULTRY SCIENCE
巻 47,
号 1,
p. 53-56,
発行日 2010-01-25
|
| 抄録 |
|
|
内容記述 |
Numerous studies in mammalian species have recently been reported that many stem cells have an ability to efficiently efflux the vital DNA-binding dye Hoechst 33342, and it is called side population (SP) cells. However, few study have been reported on the avian SP cells. It could be possible that concentration of hematopoietic stem cells (HSCs) in birds since the characteristic of SIP cells should be shared in various tissues and species. In this study, we first attempted the isolation of SP cells from chicken bone marrow and the assessment by gene expression and morphologic analyses. Bone marrow cells (BMCs) were flushed from the femurs and tibias of chicks aged at 10 days with PBS. The BMCs were layered on lymphocyte separation medium and centrifuged for excluding the erythrocytes. The separated cells were adjusted to 10(6)/ml in HBSS. Hoechst 33342 were added (1.25 mu g/ml) and incubated 60 to 90 minutes at 37 degrees C. Propidium iodide was added (2 mu g/ml) to exclude dead cells. The SP cells were isolated with flow cytometer. The sorted cells were stained with May-Gruenwald Giemsa (MG) for morphological analysis and RNA was extracted for gene expression analysis. The avian SP cells which was vanished by addition verapamil counld be separated. The percentage of SP cells in chicken bone marrow was about 2.6%. The morphological analysis by MG staining indicated that the SP cells had a larger nuclear and little cytoplasm which were typical characterisation of mouse HSCs. The pattern of gene expressions (CD34, c-Kit, CD4 and CD8) in SP cells also resembled that of the mouse HSCs. These results suggested that the HSCs could be enriched from avian bone marrow cells. Together with these results, it was concluded that SP is one of powerful tools for concentration of avian stem cells. |
| 資源タイプ（コンテンツの種類） |
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| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1346-7395 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11564513 |
| 異版である |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
http://joi.jlc.jst.go.jp/JST.JSTAGE/jpsa/009052 |
| 権利 |
|
|
権利情報 |
Copyright (c) 2010 by Japan Poultry Science Association |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| WoS |
|
|
URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000273999400009 |
A_Novel_Concentrating_System_Chicken_Stem_Cells.pdf (280.4 kB)
