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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

A Novel Concentrating System of Chicken Stem Cells by Bone Marrow Side Population Cells

http://hdl.handle.net/10091/13184
http://hdl.handle.net/10091/13184
dceedd91-e436-4b69-a0f8-fa71efc04d65
名前 / ファイル ライセンス アクション
A_Novel_Concentrating_System_Chicken_Stem_Cells.pdf A_Novel_Concentrating_System_Chicken_Stem_Cells.pdf (280.4 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2011-06-20
タイトル
タイトル A Novel Concentrating System of Chicken Stem Cells by Bone Marrow Side Population Cells
言語
言語 eng
DOI
関連識別子 https://doi.org/10.2141/jpsa.009052
関連名称 10.2141/jpsa.009052
キーワード
主題 bone marrow cells, chicken, flow cytometric analysis, hematopoietic stem cells, side population
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Usui, Fumitake

× Usui, Fumitake

Usui, Fumitake

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Nakamura, Yoshiaki

× Nakamura, Yoshiaki

Nakamura, Yoshiaki

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Yamamoto, Yasuhiro

× Yamamoto, Yasuhiro

Yamamoto, Yasuhiro

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Tatsumi, Kouichi

× Tatsumi, Kouichi

Tatsumi, Kouichi

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Tominari, Kazue

× Tominari, Kazue

Tominari, Kazue

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Ono, Tamao

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Ono, Tamao

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Kagami, Hiroshi

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Kagami, Hiroshi

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信州大学研究者総覧へのリンク
氏名 Ono, Tamao
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html
信州大学研究者総覧へのリンク
氏名 Kagami, Hiroshi
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html
出版者
出版者 JAPAN POULTRY SCIENCE ASSOC
引用
内容記述 JOURNAL OF POULTRY SCIENCE. 47(1): 53-56(2010)
書誌情報 JOURNAL OF POULTRY SCIENCE

巻 47, 号 1, p. 53-56, 発行日 2010-01-25
抄録
内容記述 Numerous studies in mammalian species have recently been reported that many stem cells have an ability to efficiently efflux the vital DNA-binding dye Hoechst 33342, and it is called side population (SP) cells. However, few study have been reported on the avian SP cells. It could be possible that concentration of hematopoietic stem cells (HSCs) in birds since the characteristic of SIP cells should be shared in various tissues and species. In this study, we first attempted the isolation of SP cells from chicken bone marrow and the assessment by gene expression and morphologic analyses. Bone marrow cells (BMCs) were flushed from the femurs and tibias of chicks aged at 10 days with PBS. The BMCs were layered on lymphocyte separation medium and centrifuged for excluding the erythrocytes. The separated cells were adjusted to 10(6)/ml in HBSS. Hoechst 33342 were added (1.25 mu g/ml) and incubated 60 to 90 minutes at 37 degrees C. Propidium iodide was added (2 mu g/ml) to exclude dead cells. The SP cells were isolated with flow cytometer. The sorted cells were stained with May-Gruenwald Giemsa (MG) for morphological analysis and RNA was extracted for gene expression analysis. The avian SP cells which was vanished by addition verapamil counld be separated. The percentage of SP cells in chicken bone marrow was about 2.6%. The morphological analysis by MG staining indicated that the SP cells had a larger nuclear and little cytoplasm which were typical characterisation of mouse HSCs. The pattern of gene expressions (CD34, c-Kit, CD4 and CD8) in SP cells also resembled that of the mouse HSCs. These results suggested that the HSCs could be enriched from avian bone marrow cells. Together with these results, it was concluded that SP is one of powerful tools for concentration of avian stem cells.
資源タイプ(コンテンツの種類)
ISSN
収録物識別子タイプ ISSN
収録物識別子 1346-7395
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11564513
異版である
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://joi.jlc.jst.go.jp/JST.JSTAGE/jpsa/009052
権利
権利情報 Copyright (c) 2010 by Japan Poultry Science Association
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
WoS
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000273999400009
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