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Depletion of Primordial Germ Cells (PGCs) by X-irradiation to Extraembryonic Region of Chicken Embryos and Expression of Xenotransplanted Quail PGCs
http://hdl.handle.net/10091/13182
http://hdl.handle.net/10091/131824c703112-7034-4c33-956d-6e862cabd9ba
| 名前 / ファイル | ライセンス | アクション |
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Depletion_Primordial_Germ_Cells_PGCs.pdf (527.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||||
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| 公開日 | 2011-06-20 | |||||||||||||||||||||||
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| タイトル | Depletion of Primordial Germ Cells (PGCs) by X-irradiation to Extraembryonic Region of Chicken Embryos and Expression of Xenotransplanted Quail PGCs | |||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||
| DOI | ||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.2141/jpsa.46.136 | |||||||||||||||||||||||
| 関連名称 | 10.2141/jpsa.46.136 | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | chicken, chimera, primordial germ cell, quail, soft x-ray | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| タイプ | journal article | |||||||||||||||||||||||
| 著者 |
Atsumi, Yusuke
× Atsumi, Yusuke
× Yazawa, Shigenobu
× Usui, Fumitake
× Nakamura, Yoshiaki
× Yamamoto, Yasuhiro
× Tagami, Takahiro
× Hiramatsu, Kohzy
× Kagami, Hiroshi
× Ono, Tamao
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| 信州大学研究者総覧へのリンク | ||||||||||||||||||||||||
| 氏名 | Hiramatsu, Kohzy | |||||||||||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.yeTmPpkh.html | |||||||||||||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||||||||||||
| 氏名 | Kagami, Hiroshi | |||||||||||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html | |||||||||||||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||||||||||||
| 氏名 | Ono, Tamao | |||||||||||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html | |||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||
| 出版者 | JAPAN POULTRY SCIENCE ASSOC | |||||||||||||||||||||||
| 引用 | ||||||||||||||||||||||||
| 内容記述 | JOURNAL OF POULTRY SCIENCE. 46(2): 136-143(2009) | |||||||||||||||||||||||
| 書誌情報 |
JOURNAL OF POULTRY SCIENCE 巻 46, 号 2, p. 136-143, 発行日 2009-04-25 |
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| 抄録 | ||||||||||||||||||||||||
| 内容記述 | The generation of germline chimeras by the transfer of primordial germ cells (PGCs) requires incorporation of the PGCs of the donor into the gonadal tissue of the recipient embryo. We investigated the utility of soft x-irradiation with application of a lead (12-3 x 0.25 mm, similar to 0.1 g) shield to the embryo proper for the production of chicken-quail germline chimeras. Chicken embryos shielded during irradiation for 120 s (similar to 7.2 Gy) at stages 13 to 17 showed a hatchability of 35% (106/301). whereas the hatchability of unshielded embryos was 26% (27/105). The relative Population of gonadal PGCs Lit stage 30 for embryos irradiated at stage 13 with or Without shielding was 13 and 5%. respectively, of the value for nonirradiated controls. Chicken embryos irradiated at stages 13 or 14 with or Without shielding and transfused with quail embryonic blood containing PGCs each exhibited similar to 130 relative population of donor PGCs in the left gonad at stage 30. Xenotransplanted hatchlings exhibited donor-derived PGCs as detected by Southern hybridization and PCR. Exposure of chicken embryos to similar to 7.2 Gy of x-radiation at stage 13 with the application of a lead shield to the embryo proper is thus a feasible approach to depletion of endogenous germ cells and the production of chicken-quail germline chimeras. | |||||||||||||||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||||||||||||||
| ISSN | ||||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 1346-7395 | |||||||||||||||||||||||
| 書誌レコードID | ||||||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA11564513 | |||||||||||||||||||||||
| 異版である | ||||||||||||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||||||||||||
| 識別子タイプ | URI | |||||||||||||||||||||||
| 関連識別子 | http://joi.jlc.jst.go.jp/JST.JSTAGE/jpsa.46.136 | |||||||||||||||||||||||
| 権利 | ||||||||||||||||||||||||
| 権利情報 | Copyright (c) 2008 by Japan Poultry Science Association | |||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||
| WoS | ||||||||||||||||||||||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000265555300011 | |||||||||||||||||||||||
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Atsumi, Yusuke, Yazawa, Shigenobu, Usui, Fumitake, Nakamura, Yoshiaki, Yamamoto, Yasuhiro, Tagami, Takahiro, Hiramatsu, Kohzy, Kagami, Hiroshi, Ono, Tamao, 2009, Depletion of Primordial Germ Cells (PGCs) by X-irradiation to Extraembryonic Region of Chicken Embryos and Expression of Xenotransplanted Quail PGCs: JAPAN POULTRY SCIENCE ASSOC, 136–143 p.
