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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

Depletion of Primordial Germ Cells (PGCs) by X-irradiation to Extraembryonic Region of Chicken Embryos and Expression of Xenotransplanted Quail PGCs

http://hdl.handle.net/10091/13182
http://hdl.handle.net/10091/13182
4c703112-7034-4c33-956d-6e862cabd9ba
名前 / ファイル ライセンス アクション
Depletion_Primordial_Germ_Cells_PGCs.pdf Depletion_Primordial_Germ_Cells_PGCs.pdf (527.6 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2011-06-20
タイトル
タイトル Depletion of Primordial Germ Cells (PGCs) by X-irradiation to Extraembryonic Region of Chicken Embryos and Expression of Xenotransplanted Quail PGCs
言語
言語 eng
DOI
関連識別子 https://doi.org/10.2141/jpsa.46.136
関連名称 10.2141/jpsa.46.136
キーワード
主題 chicken, chimera, primordial germ cell, quail, soft x-ray
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Atsumi, Yusuke

× Atsumi, Yusuke

Atsumi, Yusuke

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Yazawa, Shigenobu

× Yazawa, Shigenobu

Yazawa, Shigenobu

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Usui, Fumitake

× Usui, Fumitake

Usui, Fumitake

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Nakamura, Yoshiaki

× Nakamura, Yoshiaki

Nakamura, Yoshiaki

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Yamamoto, Yasuhiro

× Yamamoto, Yasuhiro

Yamamoto, Yasuhiro

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Tagami, Takahiro

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Tagami, Takahiro

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Hiramatsu, Kohzy

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Hiramatsu, Kohzy

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Kagami, Hiroshi

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Kagami, Hiroshi

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Ono, Tamao

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Ono, Tamao

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信州大学研究者総覧へのリンク
氏名 Hiramatsu, Kohzy
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.yeTmPpkh.html
信州大学研究者総覧へのリンク
氏名 Kagami, Hiroshi
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html
信州大学研究者総覧へのリンク
氏名 Ono, Tamao
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html
出版者
出版者 JAPAN POULTRY SCIENCE ASSOC
引用
内容記述 JOURNAL OF POULTRY SCIENCE. 46(2): 136-143(2009)
書誌情報 JOURNAL OF POULTRY SCIENCE

巻 46, 号 2, p. 136-143, 発行日 2009-04-25
抄録
内容記述 The generation of germline chimeras by the transfer of primordial germ cells (PGCs) requires incorporation of the PGCs of the donor into the gonadal tissue of the recipient embryo. We investigated the utility of soft x-irradiation with application of a lead (12-3 x 0.25 mm, similar to 0.1 g) shield to the embryo proper for the production of chicken-quail germline chimeras. Chicken embryos shielded during irradiation for 120 s (similar to 7.2 Gy) at stages 13 to 17 showed a hatchability of 35% (106/301). whereas the hatchability of unshielded embryos was 26% (27/105). The relative Population of gonadal PGCs Lit stage 30 for embryos irradiated at stage 13 with or Without shielding was 13 and 5%. respectively, of the value for nonirradiated controls. Chicken embryos irradiated at stages 13 or 14 with or Without shielding and transfused with quail embryonic blood containing PGCs each exhibited similar to 130 relative population of donor PGCs in the left gonad at stage 30. Xenotransplanted hatchlings exhibited donor-derived PGCs as detected by Southern hybridization and PCR. Exposure of chicken embryos to similar to 7.2 Gy of x-radiation at stage 13 with the application of a lead shield to the embryo proper is thus a feasible approach to depletion of endogenous germ cells and the production of chicken-quail germline chimeras.
資源タイプ(コンテンツの種類)
ISSN
収録物識別子タイプ ISSN
収録物識別子 1346-7395
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11564513
異版である
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://joi.jlc.jst.go.jp/JST.JSTAGE/jpsa.46.136
権利
権利情報 Copyright (c) 2008 by Japan Poultry Science Association
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
WoS
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000265555300011
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Cite as

Atsumi, Yusuke, Yazawa, Shigenobu, Usui, Fumitake, Nakamura, Yoshiaki, Yamamoto, Yasuhiro, Tagami, Takahiro, Hiramatsu, Kohzy, Kagami, Hiroshi, Ono, Tamao, 2009, Depletion of Primordial Germ Cells (PGCs) by X-irradiation to Extraembryonic Region of Chicken Embryos and Expression of Xenotransplanted Quail PGCs: JAPAN POULTRY SCIENCE ASSOC, 136–143 p.

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