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Sequence analysis of their arg operons identified three point mutations (argR123, argG92(up), and argG45) in one producer and one point mutation (argB26 or argB31) in each of the other two producers. Reconstitution of the former three mutations or of each argB mutation on a wild-type genome led to no production. Combined introduction of argB26 or argB31 with argR123 into a wild type gave rise to arginine/citrulline production. When argR123 was replaced by an argR-deleted mutation (Delta argR), the production was further increased. The best mutation set, Delta argR and argB26, was used to screen for the highest productivity in the backgrounds of different wild-type strains of C. glutamicum. This yielded a robust producer, RB, but the production was still one-third of that of the best classical producer. Transcriptome analysis revealed that the arg operon of the classical producer was much more highly upregulated than that of strain RB. Introduction of leuC456, a mutation derived from a classical L-lysine producer and provoking global induction of the amino acid biosynthesis genes, including the arg operon, into strain RB led to increased production but incurred retarded fermentation. On the other hand, replacement of the chromosomal argB by heterologous Escherichia coli argB, natively insensitive to arginine, caused a threefold-increased production without retardation, revealing that the limitation in strain RB was the activity of the argB product. To overcome this, in addition to argB26, the argB31 mutation was introduced into strain RB, which caused higher deregulation of the enzyme and resulted in dramatically increased production, like the strain with E. coli argB. 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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

Reengineering of a Corynebacterium glutamicum L-Arginine and L-Citrulline Producer

http://hdl.handle.net/10091/2983
http://hdl.handle.net/10091/2983
5ce44b97-bb2d-46f7-8b91-c0e339450cde
名前 / ファイル ライセンス アクション
arginin_and_citrulline_producer.pdf arginin_and_citrulline_producer.pdf (439.0 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2009-05-08
タイトル
言語 en
タイトル Reengineering of a Corynebacterium glutamicum L-Arginine and L-Citrulline Producer
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Ikeda, M

× Ikeda, M

WEKO 32899

Ikeda, M

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Mitsuhashi, S

× Mitsuhashi, S

WEKO 32900

Mitsuhashi, S

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Tanaka, K

× Tanaka, K

WEKO 32901

Tanaka, K

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Hayashi, M

× Hayashi, M

WEKO 32902

Hayashi, M

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信州大学研究者総覧へのリンク
氏名 Ikeda, M
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html
出版者
出版者 American Society for Microbiology
引用
内容記述タイプ Other
内容記述 Applied and Environmental Microbiology. 75(6):1635-1641 (2009)
書誌情報 Applied and Environmental Microbiology

巻 75, 号 6, p. 1635-1641, 発行日 2009-03
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0099-2240
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00543249
PubMed
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/19139237/
関連名称 19139237
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1128/AEM.02027-08
関連名称 10.1128/AEM.02027-08
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000263920900021
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