{"_buckets": {"deposit": "6d3e89e0-96ac-4e92-a7bc-96d2f63f1b39"}, "_deposit": {"id": "10419", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "10419"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00010419"}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-01-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1-2", "bibliographicPageEnd": "244", "bibliographicPageStart": "239", "bibliographicVolumeNumber": "649", "bibliographic_titles": [{"bibliographic_title": "MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS"}]}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_31": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\uff1amime\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS. 649(1-2): 239-244 (2008)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Takeno, S", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html"}, {"subitem_link_text": "Ikeda, M", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000252795800025"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "ELSEVIER SCIENCE BV"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "18037338"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=18037338", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/j.mrgentox.2007.10.003"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.1016/j.mrgentox.2007.10.003", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1383-5718", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1383-5718", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11116545", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "mutagenic specificity; N-methyl-N \u0027-nitro-N-nitrosoguanidine; strain improvement; Corynebacterium glutamicum"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "L-LYSINE PRODUCTION; AMINO-ACID PRODUCTION; 3-DEOXY-D-ARABINO-HEPTULOSONATE-7-PHOSPHATE SYNTHASE; FEEDBACK INHIBITION; SERRATIA-MARCESCENS; ESCHERICHIA-COLI; GENOME; MUTANT; OVERPRODUCTION; ASPARTOKINASE"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Mutations induced. by classical whole-cell mutagenesis using N-methyl-N\u0027-nitro-N-nitrosoguanidine (NTG) were determined for all genes of pathways from glucose to L-lysine in an industrial L-lysine producer of Corynebacterium glutamicum. A total of 50 mutations with a genome-wide distribution were identified and characterized for mutational types and mutagenic specificities. Those mutations were all point mutations with single-base substitutions and no deletions, frame shifts, and insertions were found. Among six possible types of base substitutions, the mutations consisted of only two types: 47 G.C --\u003e A.T transitions and three A.T --\u003e G.C transitions with no transversion. The findings indicate a limited repertoire of amino acid substitutions by classical NTG multagenesis and thus raise a new possibility of further improving industrial strains by optimizing key mutations through PCR-mediated site-directed mutagenesis. (C) 2007 Elsevier B.V. All rights reserved."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ohnishi,  J"}], "nameIdentifiers": [{"nameIdentifier": "32956", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mizoguchi,  H"}], "nameIdentifiers": [{"nameIdentifier": "32957", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takeno,  S"}], "nameIdentifiers": [{"nameIdentifier": "32958", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ikeda,  M"}], "nameIdentifiers": [{"nameIdentifier": "32959", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "mutation_research.pdf", "filesize": [{"value": "151.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 151600.0, "url": {"label": "mutation_research.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/10419/files/mutation_research.pdf"}, "version_id": "a8d0ee84-268b-4057-bbc4-87616d0ac244"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Characterization of mutations induced by N-methyl-N \u0027-nitro-N-nitrosoguanidine in an industrial Corynebacterium glutamicum strain", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Characterization of mutations induced by N-methyl-N \u0027-nitro-N-nitrosoguanidine in an industrial Corynebacterium glutamicum strain"}]}, "item_type_id": "6", "owner": "1", "path": ["1016/1017"], "permalink_uri": "http://hdl.handle.net/10091/1015", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2008-03-31"}, "publish_date": "2008-03-31", "publish_status": "0", "recid": "10419", "relation": {}, "relation_version_is_last": true, "title": ["Characterization of mutations induced by N-methyl-N \u0027-nitro-N-nitrosoguanidine in an industrial Corynebacterium glutamicum strain"], "weko_shared_id": null}