{"_buckets": {"deposit": "4cbedccd-589d-404b-9364-f01ae491d453"}, "_deposit": {"id": "10734", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "10734"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00010734"}, "item_10_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\uff08\u5225\u8a00\u8a9e\u7b49\uff09\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Influence of the Male Preputial Gland to the Acceleration of Oestrus in the Laboratory Mouse."}]}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1983-12-26", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "154", "bibliographicPageStart": "143", "bibliographicVolumeNumber": "20", "bibliographic_titles": [{"bibliographic_title": "\u4fe1\u5dde\u5927\u5b66\u8fb2\u5b66\u90e8\u7d00\u8981"}]}]}, "item_10_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Grooping of female laboratory mice in the absence of males induce to suppression of oestrus cycle (Whitten effect). Prolonged diestrus in grouped females may be overcome and oestrus synchronized by the presence of males, a phenomenon linked topheromonal properties of male odor. CHIPMAN and ALBRECHT reported that the oestrous-accelerating pheromone is closely related to and/or augmented by the preputial gland secretion. However, MCKINNEY reported that odor from male preputial did not accelerate or induce oestrus in grouped females. The present study was conducted to examine further the effects of grouping on oestrus in mice exposed continuously to the stimulus of preputial gland ordors. The present study was initiated with two objectives : (1) to obtain an efficient of the urinary pheromone, and (2) to determine if the pheromone is present in preputial gland. Mice used were ICR mature virgin females. They were maintained on our laboratory, fed and water \"ad libitum\" and kept in an environment maintained at 20\u00b11\u2103 with 12hr. of artificial light daily. In Exp. 1, 7 weeks-old females were grouped ten per cage, the dimensions of which were 17 \u00d7 30 \u00d7 10cm, for 8 days to cause inhibition of oestrus (Fig. 1). The exposure animals were 6 groups : (1) no urine, (2) intact male, (3) castrate male (4) remoevd preputial gland male, (5) ovariectomized female + testosterone 195\u03bcg/0.1ml/day, (6) ovariectomized, removed critoris gland female + testosterone which was haused individually in 10 \u00d7 16 \u00d7 10cm cages. The exposure animals were placed on the subject female cages. Frequency of oestrous following exposure with a male or fimale is shown in Fig. 2, in which (2), (4), (5) and (6) groups differed significantly (P \u003c 0.05)from (1) and (3) groups. These results were suggested that male preputial gland did not accelerate oestrus in grouped female. In Exp. 2, the exposure odour were 3 groups : (1) preputial homogenate (2) intact male urine (3) saline solution. Frequency of oestrous following exposure with these odour is shown in Fig. 3. The observed frequency of oestrus in (2) intact male urine differed significantly (P \u003c 0.05) from preputial homogenate and saline solution. Topical application of preputial gland homogenate failed to promote oetsrous among grouped females. Kidneys of males are havier than those of females and kidney weight is increased by androgen treatment (Fig. 4). In histological observation, the preputial glands are androgen-dependency (Fig. 5 and 6). Furthermore, Bowman\u0027s capsules with cuboidal epithelium were found in 74% and 44% in the kidney of adult intact male and castrated male, respectively (Fig. 8 and 9). 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