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Neurospora sp.の生産するプロテアーゼインヒビターの諸性質
http://hdl.handle.net/10091/2309
http://hdl.handle.net/10091/2309610fdd6b-5786-4814-964c-9f6cc4c24eca
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Agriculture13-02-02.pdf (341.6 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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公開日 | 2009-03-03 | |||||||||
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タイトル | Neurospora sp.の生産するプロテアーゼインヒビターの諸性質 | |||||||||
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タイトル | Some properties of a protease inhibitor produced by Neurospora sp. | |||||||||
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言語 | jpn | |||||||||
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資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||
タイプ | departmental bulletin paper | |||||||||
著者 |
大谷, 元
× 大谷, 元
× 鴇田, 文三郎
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出版者 | ||||||||||
出版者 | 信州大学農学部 | |||||||||
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内容記述 | 信州大学農学部紀要 13(2): 131-136(1976) | |||||||||
書誌情報 |
信州大学農学部紀要 巻 13, 号 2, p. 131-136, 発行日 1976-12-25 |
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内容記述 | For the purpose to obtain fungi which are capable of producing trypsin inhi-bitors, screening test was carried out. A strain, which belongs to the genus of Neurospora was obtained, and production of the inhibitor by the strain and some properties of the inhibitor produced were examined. Results obtained were summa-rized as follows : 1) The strain produced the inhibitor maximally, when it was cultivated with shaking in a medium containing 2.0% sucrose, 2.0% peptone, 1.0% yeast extracts, 0.1% KH₂PO₄ and 0.1% MgSO₄・7H₂O (pH 5.0) at 30 C for 7 days. 2) The inhibitor was partially purified with 80% acetone, 70% saturated ammonium sulfate and 50% ethanol. 3) Molecular weight of the inhibitor was speculated to be 10000 to 30000. The inhibitor was stable both in acid and neutral solutions, and unstable in an alkaline solution. Fifty percent of activity of the inhibitor was inactivated by boiling for 5 minutes in 0.1 M phosphate buffer, pH 7.0 and more than 90% was inactivated by boiling for 30 minutes. 4) The inhibitor inhibited Nagase, papain and Pronase as well as trypsin, but not a-chymotrypsin and pepsin. 5) The inhibitor obtained in this experiment was quite different from other protease inhibitors from microorganisms in their properties. | |||||||||
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内容記述 | Article | |||||||||
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収録物識別子タイプ | ISSN | |||||||||
収録物識別子 | 0583-0621 | |||||||||
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収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AN00121352 | |||||||||
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出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |