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  1. 070 農学部, 大学院農学研究科
  2. 0702 紀要 ・刊行物
  3. 07021 信州大学農学部紀要
  4. Vol. 05

タマネギ灰色腐敗病菌(Botrytis allii Munn)の生産するペクチン分解酵素の諸性質

http://hdl.handle.net/10091/2238
http://hdl.handle.net/10091/2238
bbe5f6d7-2673-4386-b155-6d986010cd4a
名前 / ファイル ライセンス アクション
Agriculture05-02-03.pdf Agriculture05-02-03.pdf (398.4 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2009-03-03
タイトル
言語 ja
タイトル タマネギ灰色腐敗病菌(Botrytis allii Munn)の生産するペクチン分解酵素の諸性質
タイトル
言語 en
タイトル Some Properties of Pectic Enzymes Produced by Botrytis allii Munn in Vitro
言語
言語 jpn
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 田端, 信一郎

× 田端, 信一郎

WEKO 34134

田端, 信一郎

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田部, 真

× 田部, 真

WEKO 34135

田部, 真

Search repository
出版者
出版者 信州大学農学部
引用
内容記述タイプ Other
内容記述 信州大学農学部紀要 5(2): 77-84(1968)
書誌情報 信州大学農学部紀要

巻 5, 号 2, p. 77-84, 発行日 1968-12-25
抄録
内容記述タイプ Abstract
内容記述 Botrytis allii causes a soft rot of onion bulb tissues. In rotted tissues the degradation of pectic materials present in the cell walls had been observed histo-chemically. Therefore, it is suggested that this pathogen can produces pectic enzymes. The present investigation was studied in vitro on the production of extracellular pectic enzymes by B. allii and some properties of these enzymes. The results are as follows. 1. The healthy and dead tissues of onion bulbs incubated in culture filtrates (crude enzyme solution) of B. allii were softened strongly at 25℃~30℃ and pH 5.0. 2. Pectin methylesterase (PME), polygalacturonase (PG), and polymethylgalacturonase (PMG) had an optimum pH for activity of 4.0, 5.0, and 4.5, and optimum temperatures were 50, 60, and 45℃. 3. Enzyme activities of dialyzed enzyme solution being measured, PMG activity was similar to that of crude enzyme solution, but PME and PG had a half activity of it. The reduced activities did not recovered with addition of metal ions to reaction mixtures. 4. It was concluded from the results of measuring the viscosity and free aldehyde groups in reaction mixtures that PG and PMG hydrolyzing α-1,4 glycosidic bond were endo type. 5. In the time course study of the growth of B. allii the maximum growth was indicated in the liquid culture of 8 days and then, declined in dry weight. PME and PG activity of culture filtrates increased with increse of the mycelial growth and decreased after the maximum growth. PMG activtiy increased similarly but was maintained for a period of time after weight increase ceased. 6. B. allii produced these pectic enzymes adaptively when pectic substrates were present in culture medium.
言語 en
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0583-0621
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00121352
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
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