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Single or double mutations did not prevent normal sporulation, but did affect germination. Germination was severely inhibited by the double mutation of sleB and cwlD. The quality of natto made with the sleB cwlD double mutant was tested, and the amounts of glutamic acid and ammonia were very similar to those in the wild type. The possibility of industrial development of natto containing a reduced number of viable spores is presented."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mitsui,  N"}], "nameIdentifiers": [{"nameIdentifier": "35573", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Murasawa,  H"}], "nameIdentifiers": [{"nameIdentifier": "35574", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sekiguchi,  J"}], "nameIdentifiers": [{"nameIdentifier": "35575", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Development_of_Natto_with_Germination-Defective-Mutants.pdf", "filesize": [{"value": "565.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 565300.0, "url": {"label": "Development_of_Natto_with_Germination-Defective-Mutants.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/11448/files/Development_of_Natto_with_Germination-Defective-Mutants.pdf"}, "version_id": "11878b53-5eb2-4c4a-88e5-4e84ec198d02"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Development of natto with germination-defective mutants of Bacillus subtilis (natto)", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Development of natto with germination-defective mutants of Bacillus subtilis (natto)"}]}, "item_type_id": "6", "owner": "1", "path": ["1162/1163"], "permalink_uri": "http://hdl.handle.net/10091/3213", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2009-07-24"}, "publish_date": "2009-07-24", "publish_status": "0", "recid": "11448", "relation": {}, "relation_version_is_last": true, "title": ["Development of natto with germination-defective mutants of Bacillus subtilis (natto)"], "weko_shared_id": null}