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B. subtilis CwlK ( YcdD) exhibits high sequence similarity with the peptidoglycan hydrolytic L, D- endopeptidase ( PLY500) of Listeria monocytogenes phage and CwlK has the VanY motif which is a D- alanyl- D- alanine carboxypeptidase ( Pfam: http:// www. sanger. ac. uk/ Software/ Pfam/). The beta- galactosidase activity observed on cwlK- lacZ fusion indicated that the cwlK gene was expressed during the vegetative growth phase, and Western blotting suggested that CwlK seems to be localized in the membrane. Truncated CwlK fused with a histidinetag ( h- Delta CwlK) was produced in Escherichia coli and puriWed on a nickel column. The h- Delta CwlK protein hydrolyzed the peptidoglycan of B. subtilis, and the optimal pH, temperature and NaCl concentration for h- Delta CwlK were pH 6.5, 37 C, and 0 M, respectively. Interestingly, h- Delta CwlK could hydrolyze the linkage of L- alanine- D- glutamic acid in the stem of the peptidoglycan, however, this enzyme could not hydrolyze the linkage of D- alanine- D- alanine, suggesting that CwlK is an L, D- endopeptidase not a D- carboxypeptidase. CwlK could not hydrolyze polyglutamate from B. natto or peptidoglycan of Staphylococcus aureus. This is the Wrst report describing the characterization of an L, D- endopeptidase in B. subtilis and also the Wrst report in bacteria of the characterization of a PLY500 family protein encoded in chromosomal DNA."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Fukushima, T"}], "nameIdentifiers": [{"nameIdentifier": "35593", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yao, Y"}], "nameIdentifiers": [{"nameIdentifier": "35594", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitajima, T"}], "nameIdentifiers": [{"nameIdentifier": "35595", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamamoto, H"}], "nameIdentifiers": [{"nameIdentifier": "35596", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sekiguchi, J"}], "nameIdentifiers": [{"nameIdentifier": "35597", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "CwlK.pdf", "filesize": [{"value": "408.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 408600.0, "url": {"label": "CwlK.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/11453/files/CwlK.pdf"}, "version_id": "39d2f887-198b-4095-a711-86663a39ba47"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Characterization of new L,D-endopeptidase gene product CwlK (previous YcdD) that hydrolyzes peptidoglycan in Bacillus subtilis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Characterization of new L,D-endopeptidase gene product CwlK (previous YcdD) that hydrolyzes peptidoglycan in Bacillus subtilis", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1163"], "permalink_uri": "http://hdl.handle.net/10091/571", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-11-20"}, "publish_date": "2007-11-20", "publish_status": "0", "recid": "11453", "relation": {}, "relation_version_is_last": true, "title": ["Characterization of new L,D-endopeptidase gene product CwlK (previous YcdD) that hydrolyzes peptidoglycan in Bacillus subtilis"], "weko_shared_id": -1}
Characterization of new L,D-endopeptidase gene product CwlK (previous YcdD) that hydrolyzes peptidoglycan in Bacillus subtilis
http://hdl.handle.net/10091/571
http://hdl.handle.net/10091/571df9e8262-8f3a-445c-b5e7-3a8073f64d76
名前 / ファイル | ライセンス | アクション |
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CwlK.pdf (408.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-11-20 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Characterization of new L,D-endopeptidase gene product CwlK (previous YcdD) that hydrolyzes peptidoglycan in Bacillus subtilis | |||||
言語 | ||||||
言語 | eng | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1007/s00438-007-0255-8 | |||||
関連名称 | 10.1007/s00438-007-0255-8 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Fukushima, T
× Fukushima, T× Yao, Y× Kitajima, T× Yamamoto, H× Sekiguchi, J |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Yamamoto, H | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uhDNjekV.html | |||||
出版者 | ||||||
出版者 | SPRINGER | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | MOLECULAR GENETICS AND GENOMICS. 278(4): 371-383 (2007) | |||||
書誌情報 |
Molecular genetics and genomics 巻 278, 号 4, p. 371-383, 発行日 2007-10 |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The original publication is available at www.springerlink.com | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1617-4615 | |||||
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収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11547886 | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
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