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B. subtilis CwlK ( YcdD) exhibits high sequence similarity with the peptidoglycan hydrolytic L, D- endopeptidase ( PLY500) of Listeria monocytogenes phage and CwlK has the VanY motif which is a D- alanyl- D- alanine carboxypeptidase ( Pfam: http:// www. sanger. ac. uk/ Software/ Pfam/). The beta- galactosidase activity observed on cwlK- lacZ fusion indicated that the cwlK gene was expressed during the vegetative growth phase, and Western blotting suggested that CwlK seems to be localized in the membrane. Truncated CwlK fused with a histidinetag ( h- Delta CwlK) was produced in Escherichia coli and puriWed on a nickel column. The h- Delta CwlK protein hydrolyzed the peptidoglycan of B. subtilis, and the optimal pH, temperature and NaCl concentration for h- Delta CwlK were pH 6.5, 37 C, and 0 M, respectively. 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  1. 090 大学院理工学系研究科, 大学院総合工学系研究科
  2. 0901 学術論文

Characterization of new L,D-endopeptidase gene product CwlK (previous YcdD) that hydrolyzes peptidoglycan in Bacillus subtilis

http://hdl.handle.net/10091/571
df9e8262-8f3a-445c-b5e7-3a8073f64d76
名前 / ファイル ライセンス アクション
CwlK.pdf CwlK.pdf (408.6 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2007-11-20
タイトル
言語 en
タイトル Characterization of new L,D-endopeptidase gene product CwlK (previous YcdD) that hydrolyzes peptidoglycan in Bacillus subtilis
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Fukushima, T

× Fukushima, T

WEKO 35593

Fukushima, T

Search repository
Yao, Y

× Yao, Y

WEKO 35594

Yao, Y

Search repository
Kitajima, T

× Kitajima, T

WEKO 35595

Kitajima, T

Search repository
Yamamoto, H

× Yamamoto, H

WEKO 35596

Yamamoto, H

Search repository
Sekiguchi, J

× Sekiguchi, J

WEKO 35597

Sekiguchi, J

Search repository
信州大学研究者総覧へのリンク
氏名 Yamamoto, H
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.uhDNjekV.html
出版者
出版者 SPRINGER
引用
内容記述タイプ Other
内容記述 MOLECULAR GENETICS AND GENOMICS. 278(4): 371-383 (2007)
書誌情報 Molecular genetics and genomics

巻 278, 号 4, p. 371-383, 発行日 2007-10
内容記述
内容記述タイプ Other
内容記述 The original publication is available at www.springerlink.com
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 1617-4615
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11547886
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1007/s00438-007-0255-8
関連名称
関連名称 10.1007/s00438-007-0255-8
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000249578100003
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