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  1. 040 理学部
  2. 0401 学術論文

Enzymatic flow injection method for rapid determination of choline in urine with electrochemiluminescence detection

http://hdl.handle.net/10091/10722
http://hdl.handle.net/10091/10722
0e23c177-09a9-4254-8ee3-e0edd70bb52d
名前 / ファイル ライセンス アクション
Enzymatic_Flow_Injection_Method.pdf Enzymatic_Flow_Injection_Method.pdf (342.1 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2010-10-21
タイトル
タイトル Enzymatic flow injection method for rapid determination of choline in urine with electrochemiluminescence detection
言語
言語 eng
DOI
関連識別子 https://doi.org/10.1016/j.bioelechem.2009.12.005
関連名称 10.1016/j.bioelechem.2009.12.005
キーワード
主題 Electrochemiluminescence, Flow injection, Enzyme reactor, Choline, Human urine
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Jin, Jiye

× Jin, Jiye

Jin, Jiye

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Muroga, Masahiro

× Muroga, Masahiro

Muroga, Masahiro

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Takahashi, Fumiki

× Takahashi, Fumiki

Takahashi, Fumiki

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Nakamura, Toshio

× Nakamura, Toshio

Nakamura, Toshio

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信州大学研究者総覧へのリンク
氏名 Jin, Jiye
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.jUThymkV.html
出版者
出版者 ELSEVIER SCIENCE SA
引用
内容記述 Bioelectrochemistry. 79(1):147-151 (2010)
書誌情報 Bioelectrochemistry

巻 79, 号 1, p. 147-151, 発行日 2010-08
抄録
内容記述 In order to determine trace choline in human urine, a flow injection analysis (FIA) system has been developed by coupling an enzyme reactor with an electrochemiluminescence (ECL) detector. The enzyme reactor is prepared by covalently immobilizing choline oxidase (ChOx) onto the aminopropyl-controlled pore glass beads, which are then carefully packed into a micro column. The enzyme reactor catalyzes the production of hydrogen peroxide that is in direct proportion to the concentration of choline. In this study, the enzymatically produced hydrogen peroxide was detected by an ECL detector positioned at the down stream of an enzyme react:or based on the luminol/H2O2 ECL system. Under the optimized condition, the enzymatic FIA/ECL provided high sensitivity for the determination of choline with the detection limit as low as 0.05 mu M (absolute detection limit was at sub pmol level). The method was successfully applied in the determination of choline in the samples of human urine, and the analytical results were in good agreement with those obtained by using the microbore HPLC with an immobilized enzyme reactor-electrochemical detection system.
資源タイプ(コンテンツの種類)
ISSN
収録物識別子タイプ ISSN
収録物識別子 1567-5394
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11437254
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000278666300024
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Cite as

Jin, Jiye, Muroga, Masahiro, Takahashi, Fumiki, Nakamura, Toshio, 2010, Enzymatic flow injection method for rapid determination of choline in urine with electrochemiluminescence detection: ELSEVIER SCIENCE SA, 147–151 p.

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