WEKO3
-
RootNode
アイテム
Enzymatic flow injection method for rapid determination of choline in urine with electrochemiluminescence detection
http://hdl.handle.net/10091/10722
http://hdl.handle.net/10091/107220e23c177-09a9-4254-8ee3-e0edd70bb52d
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
公開日 | 2010-10-21 | |||||||||||||
タイトル | ||||||||||||||
タイトル | Enzymatic flow injection method for rapid determination of choline in urine with electrochemiluminescence detection | |||||||||||||
言語 | ||||||||||||||
言語 | eng | |||||||||||||
DOI | ||||||||||||||
関連識別子 | https://doi.org/10.1016/j.bioelechem.2009.12.005 | |||||||||||||
関連名称 | 10.1016/j.bioelechem.2009.12.005 | |||||||||||||
キーワード | ||||||||||||||
主題 | Electrochemiluminescence, Flow injection, Enzyme reactor, Choline, Human urine | |||||||||||||
資源タイプ | ||||||||||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
タイプ | journal article | |||||||||||||
著者 |
Jin, Jiye
× Jin, Jiye
× Muroga, Masahiro
× Takahashi, Fumiki
× Nakamura, Toshio
|
|||||||||||||
信州大学研究者総覧へのリンク | ||||||||||||||
氏名 | Jin, Jiye | |||||||||||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.jUThymkV.html | |||||||||||||
出版者 | ||||||||||||||
出版者 | ELSEVIER SCIENCE SA | |||||||||||||
引用 | ||||||||||||||
内容記述 | Bioelectrochemistry. 79(1):147-151 (2010) | |||||||||||||
書誌情報 |
Bioelectrochemistry 巻 79, 号 1, p. 147-151, 発行日 2010-08 |
|||||||||||||
抄録 | ||||||||||||||
内容記述 | In order to determine trace choline in human urine, a flow injection analysis (FIA) system has been developed by coupling an enzyme reactor with an electrochemiluminescence (ECL) detector. The enzyme reactor is prepared by covalently immobilizing choline oxidase (ChOx) onto the aminopropyl-controlled pore glass beads, which are then carefully packed into a micro column. The enzyme reactor catalyzes the production of hydrogen peroxide that is in direct proportion to the concentration of choline. In this study, the enzymatically produced hydrogen peroxide was detected by an ECL detector positioned at the down stream of an enzyme react:or based on the luminol/H2O2 ECL system. Under the optimized condition, the enzymatic FIA/ECL provided high sensitivity for the determination of choline with the detection limit as low as 0.05 mu M (absolute detection limit was at sub pmol level). The method was successfully applied in the determination of choline in the samples of human urine, and the analytical results were in good agreement with those obtained by using the microbore HPLC with an immobilized enzyme reactor-electrochemical detection system. | |||||||||||||
資源タイプ(コンテンツの種類) | ||||||||||||||
ISSN | ||||||||||||||
収録物識別子タイプ | ISSN | |||||||||||||
収録物識別子 | 1567-5394 | |||||||||||||
書誌レコードID | ||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||
収録物識別子 | AA11437254 | |||||||||||||
出版タイプ | ||||||||||||||
出版タイプ | AM | |||||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||
WoS | ||||||||||||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000278666300024 |
Share
Cite as
Jin, Jiye, Muroga, Masahiro, Takahashi, Fumiki, Nakamura, Toshio, 2010, Enzymatic flow injection method for rapid determination of choline in urine with electrochemiluminescence detection: ELSEVIER SCIENCE SA, 147–151 p.
Loading...