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The artificial sex reversal was carried out successfully by the oral treatment of methylteststeron for two months after hatching out. In the sex reversed triploid ginbuna, the processes of spermatogenesis from spermatogonia to the primary spermatocyte was similar with diploid males. But thereafter though in the diploid male two different types of nuclear plate in diameter were observed, in the triploid ginbuna it was observed only the same diameter of nuclear plate. From these observations, it was concluded that the first maturation division might be omitted in these males as in females. Further, the triploid males produced the unequal diameter and DNA contents of sperms. This might be caused by unequal nuclear division during meiosis. Artificial insemination of triploid female with the sperm of sex reversed triploid male, resulted in the all triploid females. 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