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Analysis of a change in bacterial community in different environments with addition of chitin or chitosan
http://hdl.handle.net/10091/16191
http://hdl.handle.net/10091/161912f3f1b60-e986-4e89-a795-4da96fdb7496
| 名前 / ファイル | ライセンス | アクション |
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Analysis_change_bacterial_community_different_environments.pdf (781.3 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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| 公開日 | 2013-01-18 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Analysis of a change in bacterial community in different environments with addition of chitin or chitosan | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| DOI | ||||||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.jbiosc.2009.10.021 | |||||||||||||||
| 関連名称 | 10.1016/j.jbiosc.2009.10.021 | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題 | Bacterial community, PCR-DGGE, 16S rRNA gene, Chitin, Chitosan, Unculturable bacteria | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| タイプ | journal article | |||||||||||||||
| 著者 |
Sato, Kazuaki
× Sato, Kazuaki
× Azama, Yasuhito
× Nogawa, Masahiro
× Taguchi, Goro
× Shimosaka, Makoto
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| 信州大学研究者総覧へのリンク | ||||||||||||||||
| 氏名 | Nogawa, Masahiro | |||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.gNcajNnU.html | |||||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||||
| 氏名 | Taguchi, Goro | |||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html | |||||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||||
| 氏名 | Shimosaka, Makoto | |||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html | |||||||||||||||
| 出版者 | ||||||||||||||||
| 出版者 | SOC BIOSCIENCE BIOENGINEERING JAPAN | |||||||||||||||
| 引用 | ||||||||||||||||
| 内容記述 | JOURNAL OF BIOSCIENCE AND BIOENGINEERING. 109(5):472-478 (2010) | |||||||||||||||
| 書誌情報 |
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 巻 109, 号 5, p. 472-478, 発行日 2010-05 |
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| 抄録 | ||||||||||||||||
| 内容記述 | The temporal changes of a bacterial community in soil with chitin or chitosan added were analyzed by PCR-denaturing gradient gel electrophoresis (DGGE) targeting the 16S rRNA gene using total DNAs prepared from the community. Band patterns of PCR-DGGE confirmed that 31 species become predominant after the addition of chitin or chitosan. The determination of the nucleotide sequences of the bands of the 31 species indicated that 20 species belonged to the division Proteobacteria, and that the genus Celivibrio was apparently predominant among them (7/20). The 16S rRNA sequences of the 16 deduced species (16/31) showed less than 98% similarities to those of previously identified bacteria, indicating that the species were derived from unidentified bacteria. The total community DNAs extracted from bacterial cells adsorbed on the surface of flakes of chitin and chitosan placed in a river, a moat, or soil were subjected to PCR-DGGE to examine the extent of diversity of chitinolytic bacteria among different environments. The predominant species significantly differed between the chitin and chitosan placed in the river and moat, but not so much between those placed in the soil. The large difference between the diversities of the three bacterial communities indicated that a wide variety of bacteria including unidentified ones are involved in the degradation of chitin and chitosan in the above-mentioned natural environments. (C) 2009, The Society for Biotechnology, Japan. All rights reserved. | |||||||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||||||
| ISSN | ||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||
| 収録物識別子 | 1389-1723 | |||||||||||||||
| 書誌レコードID | ||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||
| 収録物識別子 | AA11307678 | |||||||||||||||
| PubMed | ||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||
| 関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=20347770 | |||||||||||||||
| 関連名称 | 20347770 | |||||||||||||||
| 権利 | ||||||||||||||||
| 権利情報 | Copyright© 2009, The Society for Biotechnology, Japan. | |||||||||||||||
| 出版タイプ | ||||||||||||||||
| 出版タイプ | AM | |||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||
| WoS | ||||||||||||||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000279089600008 | |||||||||||||||
