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Here we investigate the effects of the cell wall lytic enzymes belonging to the D,L-endo-peptidases (LytE, LytF, CwlO and CwlS) on gamma PGA production by B. subtilis (natto). gamma PGA levels in a cwlO disruptant were about twofold higher than that of the wild-type strain, whereas disruption of the lytE, lytF and cwlS genes had little effect on gamma PGA production. The molecular size of gamma PGA in the cwlO disruptant was larger than that of the wild-type strain. A complementary strain was constructed by insertion of the entire cwlO gene into the amyE locus of the CwlO mutant genome, and gamma PGA production was restored to wild-type levels in this complementary strain. 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Here we investigate the effects of the cell wall lytic enzymes belonging to the D,L-endo-peptidases (LytE, LytF, CwlO and CwlS) on gamma PGA production by B. subtilis (natto). gamma PGA levels in a cwlO disruptant were about twofold higher than that of the wild-type strain, whereas disruption of the lytE, lytF and cwlS genes had little effect on gamma PGA production. The molecular size of gamma PGA in the cwlO disruptant was larger than that of the wild-type strain. A complementary strain was constructed by insertion of the entire cwlO gene into the amyE locus of the CwlO mutant genome, and gamma PGA production was restored to wild-type levels in this complementary strain. These results indicated that the peptidoglycan degradation enzyme, CwlO, plays an important role in gamma PGA production and affects the molecular size of gamma PGA."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mitsui,  Nobuo"}], "nameIdentifiers": [{"nameIdentifier": "40636", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Murasawa,  Hisashi"}], "nameIdentifiers": [{"nameIdentifier": "40637", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sekiguchi,  Junichi"}], "nameIdentifiers": [{"nameIdentifier": "40638", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Disruption_cell_wall_lytic_enzyme_CwlO_affects.pdf", "filesize": [{"value": "841.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 841300.0, "url": {"label": "Disruption_cell_wall_lytic_enzyme_CwlO_affects.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/13377/files/Disruption_cell_wall_lytic_enzyme_CwlO_affects.pdf"}, "version_id": "2b9c0a48-71be-4018-a397-8bd183564cc6"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Bacillus subtilis (natto)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "CwlO", "subitem_subject_scheme": "Other"}, {"subitem_subject": "peptidoglycan hydrolase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "poly-gamma-glutamic acid", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Disruption of the cell wall lytic enzyme CwlO affects the amount and molecular size of poly-gamma-glutamic acid produced by Bacillus subtilis (natto)", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Disruption of the cell wall lytic enzyme CwlO affects the amount and molecular size of poly-gamma-glutamic acid produced by Bacillus subtilis (natto)", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1310"], "permalink_uri": "http://hdl.handle.net/10091/16190", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2013-01-18"}, "publish_date": "2013-01-18", "publish_status": "0", "recid": "13377", "relation": {}, "relation_version_is_last": true, "title": ["Disruption of the cell wall lytic enzyme CwlO affects the amount and molecular size of poly-gamma-glutamic acid produced by Bacillus subtilis (natto)"], "weko_shared_id": -1}