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Here we investigate the effects of the cell wall lytic enzymes belonging to the D,L-endo-peptidases (LytE, LytF, CwlO and CwlS) on gamma PGA production by B. subtilis (natto). gamma PGA levels in a cwlO disruptant were about twofold higher than that of the wild-type strain, whereas disruption of the lytE, lytF and cwlS genes had little effect on gamma PGA production. The molecular size of gamma PGA in the cwlO disruptant was larger than that of the wild-type strain. A complementary strain was constructed by insertion of the entire cwlO gene into the amyE locus of the CwlO mutant genome, and gamma PGA production was restored to wild-type levels in this complementary strain. These results indicated that the peptidoglycan degradation enzyme, CwlO, plays an important role in gamma PGA production and affects the molecular size of gamma PGA.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY. 57(1):35-43 (2011)", "subitem_description_type": "Other"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000296949000004"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "MICROBIOL RES FOUNDATION"}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.2323/jgam.57.35"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.2323/jgam.57.35", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "Copyright\u00a9 2011 The Microbiology Research Foundation"}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0022-1260", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0022-1260", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00698664", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Bacillus subtilis (natto); CwlO; peptidoglycan hydrolase; poly-gamma-glutamic acid"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "POLYGLUTAMIC ACID; HYDROLASE GENE; DEGRADATION; SEPARATION; SYNTHETASE; ENCODES; PROTEIN; GROWTH; PLAYS"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Poly-gamma-glutamic acid (gamma PGA), a polymer of glutamic acid, is a component of the viscosity substance of natto, a traditional Japanese food made from soybeans fermented with Bacillus subtilis (natto). Here we investigate the effects of the cell wall lytic enzymes belonging to the D,L-endo-peptidases (LytE, LytF, CwlO and CwlS) on gamma PGA production by B. subtilis (natto). gamma PGA levels in a cwlO disruptant were about twofold higher than that of the wild-type strain, whereas disruption of the lytE, lytF and cwlS genes had little effect on gamma PGA production. The molecular size of gamma PGA in the cwlO disruptant was larger than that of the wild-type strain. A complementary strain was constructed by insertion of the entire cwlO gene into the amyE locus of the CwlO mutant genome, and gamma PGA production was restored to wild-type levels in this complementary strain. These results indicated that the peptidoglycan degradation enzyme, CwlO, plays an important role in gamma PGA production and affects the molecular size of gamma PGA."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mitsui,  Nobuo"}], "nameIdentifiers": [{"nameIdentifier": "40636", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Murasawa,  Hisashi"}], "nameIdentifiers": [{"nameIdentifier": "40637", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sekiguchi,  Junichi"}], "nameIdentifiers": [{"nameIdentifier": "40638", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Disruption_cell_wall_lytic_enzyme_CwlO_affects.pdf", "filesize": [{"value": "841.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 841300.0, "url": {"label": "Disruption_cell_wall_lytic_enzyme_CwlO_affects.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/13377/files/Disruption_cell_wall_lytic_enzyme_CwlO_affects.pdf"}, "version_id": "2b9c0a48-71be-4018-a397-8bd183564cc6"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Bacillus subtilis (natto)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "CwlO", "subitem_subject_scheme": "Other"}, {"subitem_subject": "peptidoglycan hydrolase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "poly-gamma-glutamic acid", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Disruption of the cell wall lytic enzyme CwlO affects the amount and molecular size of poly-gamma-glutamic acid produced by Bacillus subtilis (natto)", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Disruption of the cell wall lytic enzyme CwlO affects the amount and molecular size of poly-gamma-glutamic acid produced by Bacillus subtilis (natto)", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1309/1310"], "permalink_uri": "http://hdl.handle.net/10091/16190", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2013-01-18"}, "publish_date": "2013-01-18", "publish_status": "0", "recid": "13377", "relation": {}, "relation_version_is_last": true, "title": ["Disruption of the cell wall lytic enzyme CwlO affects the amount and molecular size of poly-gamma-glutamic acid produced by Bacillus subtilis (natto)"], "weko_shared_id": -1}