Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2013-01-18 |
タイトル |
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タイトル |
Disruption of the cell wall lytic enzyme CwlO affects the amount and molecular size of poly-gamma-glutamic acid produced by Bacillus subtilis (natto) |
言語 |
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言語 |
eng |
DOI |
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関連識別子 |
https://doi.org/10.2323/jgam.57.35 |
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関連名称 |
10.2323/jgam.57.35 |
キーワード |
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主題 |
Bacillus subtilis (natto), CwlO, peptidoglycan hydrolase, poly-gamma-glutamic acid |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Mitsui, Nobuo
Murasawa, Hisashi
Sekiguchi, Junichi
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出版者 |
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出版者 |
MICROBIOL RES FOUNDATION |
引用 |
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内容記述 |
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY. 57(1):35-43 (2011) |
書誌情報 |
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
巻 57,
号 1,
p. 35-43,
発行日 2011-02
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抄録 |
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内容記述 |
Poly-gamma-glutamic acid (gamma PGA), a polymer of glutamic acid, is a component of the viscosity substance of natto, a traditional Japanese food made from soybeans fermented with Bacillus subtilis (natto). Here we investigate the effects of the cell wall lytic enzymes belonging to the D,L-endo-peptidases (LytE, LytF, CwlO and CwlS) on gamma PGA production by B. subtilis (natto). gamma PGA levels in a cwlO disruptant were about twofold higher than that of the wild-type strain, whereas disruption of the lytE, lytF and cwlS genes had little effect on gamma PGA production. The molecular size of gamma PGA in the cwlO disruptant was larger than that of the wild-type strain. A complementary strain was constructed by insertion of the entire cwlO gene into the amyE locus of the CwlO mutant genome, and gamma PGA production was restored to wild-type levels in this complementary strain. These results indicated that the peptidoglycan degradation enzyme, CwlO, plays an important role in gamma PGA production and affects the molecular size of gamma PGA. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0022-1260 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00698664 |
権利 |
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権利情報 |
Copyright© 2011 The Microbiology Research Foundation |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000296949000004 |