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  1. 080 繊維学部
  2. 0801 学術論文

Disruption of the cell wall lytic enzyme CwlO affects the amount and molecular size of poly-gamma-glutamic acid produced by Bacillus subtilis (natto)

http://hdl.handle.net/10091/16190
http://hdl.handle.net/10091/16190
7a4a93f3-b3d2-44cf-bd5e-ae008a337fdf
名前 / ファイル ライセンス アクション
Disruption_cell_wall_lytic_enzyme_CwlO_affects.pdf Disruption_cell_wall_lytic_enzyme_CwlO_affects.pdf (841.3 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2013-01-18
タイトル
言語 en
タイトル Disruption of the cell wall lytic enzyme CwlO affects the amount and molecular size of poly-gamma-glutamic acid produced by Bacillus subtilis (natto)
言語
言語 eng
キーワード
主題Scheme Other
主題 Bacillus subtilis (natto)
キーワード
主題Scheme Other
主題 CwlO
キーワード
主題Scheme Other
主題 peptidoglycan hydrolase
キーワード
主題Scheme Other
主題 poly-gamma-glutamic acid
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Mitsui, Nobuo

× Mitsui, Nobuo

WEKO 40636

Mitsui, Nobuo

Search repository
Murasawa, Hisashi

× Murasawa, Hisashi

WEKO 40637

Murasawa, Hisashi

Search repository
Sekiguchi, Junichi

× Sekiguchi, Junichi

WEKO 40638

Sekiguchi, Junichi

Search repository
出版者
出版者 MICROBIOL RES FOUNDATION
引用
内容記述タイプ Other
内容記述 JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY. 57(1):35-43 (2011)
書誌情報 JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY

巻 57, 号 1, p. 35-43, 発行日 2011-02
抄録
内容記述タイプ Abstract
内容記述 Poly-gamma-glutamic acid (gamma PGA), a polymer of glutamic acid, is a component of the viscosity substance of natto, a traditional Japanese food made from soybeans fermented with Bacillus subtilis (natto). Here we investigate the effects of the cell wall lytic enzymes belonging to the D,L-endo-peptidases (LytE, LytF, CwlO and CwlS) on gamma PGA production by B. subtilis (natto). gamma PGA levels in a cwlO disruptant were about twofold higher than that of the wild-type strain, whereas disruption of the lytE, lytF and cwlS genes had little effect on gamma PGA production. The molecular size of gamma PGA in the cwlO disruptant was larger than that of the wild-type strain. A complementary strain was constructed by insertion of the entire cwlO gene into the amyE locus of the CwlO mutant genome, and gamma PGA production was restored to wild-type levels in this complementary strain. These results indicated that the peptidoglycan degradation enzyme, CwlO, plays an important role in gamma PGA production and affects the molecular size of gamma PGA.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0022-1260
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00698664
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.2323/jgam.57.35
関連名称 10.2323/jgam.57.35
権利
権利情報 Copyright© 2011 The Microbiology Research Foundation
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000296949000004
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