Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2012-12-19 |
タイトル |
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タイトル |
The ability of freeze-dried bull spermatozoa to induce calcium oscillations and resumption of meiosis |
言語 |
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言語 |
eng |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.theriogenology.2008.08.021 |
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関連名称 |
10.1016/j.theriogenology.2008.08.021 |
キーワード |
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主題 |
Bovine spermatozoa, Calcium oscillations, Freeze-drying, Homogenous ICSI, Interspecies ICSI |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Abdalla, H
Hirabayashi, A
Hochi, S
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信州大学研究者総覧へのリンク |
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氏名 |
Hochi, S |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html |
出版者 |
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出版者 |
ELSEVIER SCIENCE INC |
引用 |
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内容記述 |
THERIOGENOLOGY. 71(3):543-552 (2009) |
書誌情報 |
THERIOGENOLOGY
巻 71,
号 3,
p. 543-552,
発行日 2009-02
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抄録 |
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内容記述 |
The objective was to investigate the ability of freeze-dried (FD) bull spermatozoa to induce calcium oscillations in mouse oocytes and meiosis resumption in in vitro-matured bovine oocytes after intracytoplasmic sperm injection (ICSI). Bull spermatozoa were freeze-dried and stored for 1 y at +25, +4, or -196 degrees C. In the first experiment, rehydrated sperm heads were microinseminated into hybrid mouse oocytes loaded with fluo-3/AM, and the kinetics of intracellular calcium concentration was monitored for I h. Repetitive increases of intracellular calcium concentration were recorded in the majority of injected oocytes, with exception of a few oocytes injected with FD sperm heads stored at +4 degrees C (11%) and +25 degrees C (8%) that exhibited a single increase or no response (non-oscillated). The proportion of oocytes that oscillated with high frequency (>= 10 spikes/h) was higher in the non-dried control group (79%; P < 0.05) than in the FD groups (58, 55, and 54% for storage at -196, +4, and +25 degrees C, respectively). In the second experiment, control and FD spermatozoa were microinseminated into in vitro-matured, denuded bovine oocytes. The oocytes were fixed and stained 12 h after ICSI. A higher proportion of bovine oocytes injected with control spermatozoa (70%; P < 0.05) resumed meiosis than those injected with +25, +4 and -196 degrees C stored FD spermatozoa (53, 48, and 57%, respectively). The proportion of ICSI oocytes that developed to the pronuclear stage (complete activation) was higher in the control group (64%; P < 0.05) than those in all the FD groups (34, 27, and 28% for storage at -196, +4, and +25 degrees C, respectively). Thus, the ability of bull spermatozoa to induce frequent intracellular calcium spikes in mouse oocytes was impaired by the process of freeze-drying, without differences among storage at +25, +4 or -196 degrees C, probably resulting in a lower proportion of bovine oocytes that resumed meiosis and/or developed to the pronuclear stage. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0093-691X |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00862972 |
PubMed |
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関連識別子 |
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=18845331 |
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関連名称 |
18845331 |
出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
WoS |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000262346700018 |