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  1. 080 繊維学部
  2. 0801 学術論文

The ability of freeze-dried bull spermatozoa to induce calcium oscillations and resumption of meiosis

http://hdl.handle.net/10091/16151
http://hdl.handle.net/10091/16151
4c01083f-af56-41f3-9be9-6462a49fa4ae
名前 / ファイル ライセンス アクション
THE71_3_543.pdf THE71_3_543.pdf (588.0 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2012-12-19
タイトル
言語 en
タイトル The ability of freeze-dried bull spermatozoa to induce calcium oscillations and resumption of meiosis
言語
言語 eng
キーワード
主題Scheme Other
主題 Bovine spermatozoa
キーワード
主題Scheme Other
主題 Calcium oscillations
キーワード
主題Scheme Other
主題 Freeze-drying
キーワード
主題Scheme Other
主題 Homogenous ICSI
キーワード
主題Scheme Other
主題 Interspecies ICSI
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Abdalla, H

× Abdalla, H

WEKO 40787

Abdalla, H

Search repository
Hirabayashi, A

× Hirabayashi, A

WEKO 40788

Hirabayashi, A

Search repository
Hochi, S

× Hochi, S

WEKO 40789

Hochi, S

Search repository
信州大学研究者総覧へのリンク
氏名 Hochi, S
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html
出版者
出版者 ELSEVIER SCIENCE INC
引用
内容記述タイプ Other
内容記述 THERIOGENOLOGY. 71(3):543-552 (2009)
書誌情報 THERIOGENOLOGY

巻 71, 号 3, p. 543-552, 発行日 2009-02
抄録
内容記述タイプ Abstract
内容記述 The objective was to investigate the ability of freeze-dried (FD) bull spermatozoa to induce calcium oscillations in mouse oocytes and meiosis resumption in in vitro-matured bovine oocytes after intracytoplasmic sperm injection (ICSI). Bull spermatozoa were freeze-dried and stored for 1 y at +25, +4, or -196 degrees C. In the first experiment, rehydrated sperm heads were microinseminated into hybrid mouse oocytes loaded with fluo-3/AM, and the kinetics of intracellular calcium concentration was monitored for I h. Repetitive increases of intracellular calcium concentration were recorded in the majority of injected oocytes, with exception of a few oocytes injected with FD sperm heads stored at +4 degrees C (11%) and +25 degrees C (8%) that exhibited a single increase or no response (non-oscillated). The proportion of oocytes that oscillated with high frequency (>= 10 spikes/h) was higher in the non-dried control group (79%; P < 0.05) than in the FD groups (58, 55, and 54% for storage at -196, +4, and +25 degrees C, respectively). In the second experiment, control and FD spermatozoa were microinseminated into in vitro-matured, denuded bovine oocytes. The oocytes were fixed and stained 12 h after ICSI. A higher proportion of bovine oocytes injected with control spermatozoa (70%; P < 0.05) resumed meiosis than those injected with +25, +4 and -196 degrees C stored FD spermatozoa (53, 48, and 57%, respectively). The proportion of ICSI oocytes that developed to the pronuclear stage (complete activation) was higher in the control group (64%; P < 0.05) than those in all the FD groups (34, 27, and 28% for storage at -196, +4, and +25 degrees C, respectively). Thus, the ability of bull spermatozoa to induce frequent intracellular calcium spikes in mouse oocytes was impaired by the process of freeze-drying, without differences among storage at +25, +4 or -196 degrees C, probably resulting in a lower proportion of bovine oocytes that resumed meiosis and/or developed to the pronuclear stage.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0093-691X
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00862972
PubMed
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=18845331
関連名称 18845331
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.theriogenology.2008.08.021
関連名称 10.1016/j.theriogenology.2008.08.021
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000262346700018
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