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A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa
http://hdl.handle.net/10091/16150
http://hdl.handle.net/10091/161508fc702f3-fa82-4912-bd54-d8e3a69d4934
| 名前 / ファイル | ライセンス | アクション |
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THE72_4_453.pdf (327.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2012-12-19 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| DOI | ||||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.theriogenology.2009.03.011 | |||||||||||||
| 関連名称 | 10.1016/j.theriogenology.2009.03.011 | |||||||||||||
| キーワード | ||||||||||||||
| 主題 | Blastocyst yield, Bovine ICSI, Ethanol, Ionomycin, Stored ovary | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| タイプ | journal article | |||||||||||||
| 著者 |
Abdalla, H
× Abdalla, H
× Shimoda, M
× Hirabayashi, M
× Hochi, S
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| 信州大学研究者総覧へのリンク | ||||||||||||||
| 氏名 | Hochi, S | |||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html | |||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | ELSEVIER SCIENCE INC | |||||||||||||
| 引用 | ||||||||||||||
| 内容記述 | THERIOGENOLOGY. 72(4):453-460 (2009) | |||||||||||||
| 書誌情報 |
THERIOGENOLOGY 巻 72, 号 4, p. 453-460, 発行日 2009-09 |
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| 抄録 | ||||||||||||||
| 内容記述 | Regardless of the presence of sperm-borne oocyte-activating factors, activation of bovine oocytes with exogenous activation stimuli is required for further development after intracytoplasmic sperm injection (ICSI). The current study was designed to develop a new activation regimen for improving the blastocyst yield after ICSI of bovine oocytes harvested from ovaries stored at 10 to 12 degrees C for 24 h. After ICSI, oocytes were treated with 5 mu M ionomycin for 5 min, 7% ethanol for 5 or 10 min, ionomycin followed by ethanol (5 or 10 min), ionomycin followed by 10 mu g/mL cycloheximide for 5 h, or ionomycin followed by 1.9 mM 6-dimethylaminopurine for 3 h. Across the activation regimens, the cleavage rates of ICSI oocytes (45% to 77%) were higher than those of parthenogenetically activated oocytes (11% to 21%; P < 0.05). Activating the ICSI oocytes with ionomycin plus ethanol improved the blastocyst yield (29% to 30%) compared with that of nontreated oocytes (12%; P < 0.05), but the other regimens did not improve the blastocyst yield (9% to 18%; P > 0.05). Higher blastocyst yields were due to increasing the proportion of ICSI oocytes that passed through the early postfertilization events until cleavage. None of the regimens have any adverse effect on the quality of the blastocysts regarding the total cell number or the proportion of the inner cell mass cells. Thus, a new activation regimen using two triggers for single calcium increase effectively improved blastocyst yield after bovine ICSI using oocytes harvested from stored ovaries. | |||||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0093-691X | |||||||||||||
| 書誌レコードID | ||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AA00862972 | |||||||||||||
| PubMed | ||||||||||||||
| 識別子タイプ | PMID | |||||||||||||
| 関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=19464048 | |||||||||||||
| 関連名称 | 19464048 | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | AM | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||
| WoS | ||||||||||||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000268901700004 | |||||||||||||
