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  1. 080 繊維学部
  2. 0801 学術論文

A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa

http://hdl.handle.net/10091/16150
8fc702f3-fa82-4912-bd54-d8e3a69d4934
名前 / ファイル ライセンス アクション
THE72_4_453.pdf THE72_4_453.pdf (327.0 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2012-12-19
タイトル
言語 en
タイトル A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa
言語
言語 eng
キーワード
主題Scheme Other
主題 Blastocyst yield
キーワード
主題Scheme Other
主題 Bovine ICSI
キーワード
主題Scheme Other
主題 Ethanol
キーワード
主題Scheme Other
主題 Ionomycin
キーワード
主題Scheme Other
主題 Stored ovary
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Abdalla, H

× Abdalla, H

WEKO 40790

Abdalla, H

Search repository
Shimoda, M

× Shimoda, M

WEKO 40791

Shimoda, M

Search repository
Hirabayashi, M

× Hirabayashi, M

WEKO 40792

Hirabayashi, M

Search repository
Hochi, S

× Hochi, S

WEKO 40793

Hochi, S

Search repository
信州大学研究者総覧へのリンク
氏名 Hochi, S
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html
出版者
出版者 ELSEVIER SCIENCE INC
引用
内容記述タイプ Other
内容記述 THERIOGENOLOGY. 72(4):453-460 (2009)
書誌情報 THERIOGENOLOGY

巻 72, 号 4, p. 453-460, 発行日 2009-09
抄録
内容記述タイプ Abstract
内容記述 Regardless of the presence of sperm-borne oocyte-activating factors, activation of bovine oocytes with exogenous activation stimuli is required for further development after intracytoplasmic sperm injection (ICSI). The current study was designed to develop a new activation regimen for improving the blastocyst yield after ICSI of bovine oocytes harvested from ovaries stored at 10 to 12 degrees C for 24 h. After ICSI, oocytes were treated with 5 mu M ionomycin for 5 min, 7% ethanol for 5 or 10 min, ionomycin followed by ethanol (5 or 10 min), ionomycin followed by 10 mu g/mL cycloheximide for 5 h, or ionomycin followed by 1.9 mM 6-dimethylaminopurine for 3 h. Across the activation regimens, the cleavage rates of ICSI oocytes (45% to 77%) were higher than those of parthenogenetically activated oocytes (11% to 21%; P < 0.05). Activating the ICSI oocytes with ionomycin plus ethanol improved the blastocyst yield (29% to 30%) compared with that of nontreated oocytes (12%; P < 0.05), but the other regimens did not improve the blastocyst yield (9% to 18%; P > 0.05). Higher blastocyst yields were due to increasing the proportion of ICSI oocytes that passed through the early postfertilization events until cleavage. None of the regimens have any adverse effect on the quality of the blastocysts regarding the total cell number or the proportion of the inner cell mass cells. Thus, a new activation regimen using two triggers for single calcium increase effectively improved blastocyst yield after bovine ICSI using oocytes harvested from stored ovaries.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0093-691X
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00862972
PubMed
関連識別子
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=19464048
関連名称
関連名称 19464048
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.theriogenology.2009.03.011
関連名称
関連名称 10.1016/j.theriogenology.2009.03.011
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000268901700004
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