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  1. 080 繊維学部
  2. 0801 学術論文

Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age

http://hdl.handle.net/10091/16148
http://hdl.handle.net/10091/16148
e41c9e6f-d2b3-45db-baf6-f5ad9bafac94
名前 / ファイル ライセンス アクション
THE74_6_1028.pdf THE74_6_1028.pdf (133.2 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2012-12-19
タイトル
タイトル Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age
言語
言語 eng
DOI
関連識別子 https://doi.org/10.1016/j.theriogenology.2010.04.033
関連名称 10.1016/j.theriogenology.2010.04.033
キーワード
主題 Bovine blastocysts, Cryotop, MVC vitrification, IVF, ICSI
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Abdalla, H

× Abdalla, H

Abdalla, H

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Shimoda, M

× Shimoda, M

Shimoda, M

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Hara, H

× Hara, H

Hara, H

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Morita, H

× Morita, H

Morita, H

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Kuwayama, M

× Kuwayama, M

Kuwayama, M

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Hirabayashi, M

× Hirabayashi, M

Hirabayashi, M

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Hochi, S

× Hochi, S

Hochi, S

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信州大学研究者総覧へのリンク
氏名 Hochi, S
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html
出版者
出版者 ELSEVIER SCIENCE INC
引用
内容記述 THERIOGENOLOGY. 74(6):1028-1035 (2010)
書誌情報 THERIOGENOLOGY

巻 74, 号 6, p. 1028-1035, 発行日 2010-10
抄録
内容記述 The objective was to investigate the effects of developmental stage (fully-expanded or expanding blastocysts) and/or age (harvested on Days 7 or 8) on post-vitrification in vitro survival of bovine blastocysts derived from intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF). Post-warming survival (re-expansion of blastocoele within 24 h) of ICSI-derived fully-expanded blastocysts (80%) was similar to that of their IVF-derived counterparts (88%). However, the ability of ICSI-derived expanding blastocysts to survive vitrification procedures (61%) was lower than that of IVF-derived blastocysts (85%; P < 0.05), although the ICSI- and IVF-derived fresh blastocysts were of similar quality. The age of the blastocysts before vitrification did not affect cryotolerance for either ICSI-derived (73 and 59% for Days 7 and 8 embryos, respectively) or IVF-derived blastocysts (86% for both Days 7 and 8 embryos). At 24 h of post-warming culture, ICSI-derived blastocysts surviving vitrification contained a higher proportion of dead cells than their IVF-derived counterparts (5-13% vs. 2-4%; P < 0.05), but these proportions were not different from those of fresh control embryos. There was an adverse effect of vitrification on the ability of blastocysts to hatch within 72 h of culture only in IVF-derived Day 8 blastocysts (41 and 70% in vitrified and fresh control groups, respectively). In conclusion, the proportion of blastocysts that survived vitrification procedures was similar for ICSI- and IVF-derived bovine blastocysts if the former were cultured to the fully-expanded stage prior to vitrification, with no significant difference between embryos harvested on Day 7 versus Day 8.
資源タイプ(コンテンツの種類)
ISSN
収録物識別子タイプ ISSN
収録物識別子 0093-691X
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00862972
PubMed
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=20580073
関連名称 20580073
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000281941500014
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Cite as

Abdalla, H, Shimoda, M, Hara, H, Morita, H, Kuwayama, M, Hirabayashi, M, Hochi, S, 2010, Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age: ELSEVIER SCIENCE INC, 1028–1035 p.

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