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  1. 080 繊維学部
  2. 0801 学術論文

Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age

http://hdl.handle.net/10091/16148
e41c9e6f-d2b3-45db-baf6-f5ad9bafac94
名前 / ファイル ライセンス アクション
THE74_6_1028.pdf THE74_6_1028.pdf (133.2 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2012-12-19
タイトル
言語 en
タイトル Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age
言語
言語 eng
キーワード
主題Scheme Other
主題 Bovine blastocysts
キーワード
主題Scheme Other
主題 Cryotop
キーワード
主題Scheme Other
主題 MVC vitrification
キーワード
主題Scheme Other
主題 IVF
キーワード
主題Scheme Other
主題 ICSI
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Abdalla, H

× Abdalla, H

WEKO 40801

Abdalla, H

Search repository
Shimoda, M

× Shimoda, M

WEKO 40802

Shimoda, M

Search repository
Hara, H

× Hara, H

WEKO 40803

Hara, H

Search repository
Morita, H

× Morita, H

WEKO 40804

Morita, H

Search repository
Kuwayama, M

× Kuwayama, M

WEKO 40805

Kuwayama, M

Search repository
Hirabayashi, M

× Hirabayashi, M

WEKO 40806

Hirabayashi, M

Search repository
Hochi, S

× Hochi, S

WEKO 40807

Hochi, S

Search repository
信州大学研究者総覧へのリンク
氏名 Hochi, S
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html
出版者
出版者 ELSEVIER SCIENCE INC
引用
内容記述タイプ Other
内容記述 THERIOGENOLOGY. 74(6):1028-1035 (2010)
書誌情報 THERIOGENOLOGY

巻 74, 号 6, p. 1028-1035, 発行日 2010-10
抄録
内容記述タイプ Abstract
内容記述 The objective was to investigate the effects of developmental stage (fully-expanded or expanding blastocysts) and/or age (harvested on Days 7 or 8) on post-vitrification in vitro survival of bovine blastocysts derived from intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF). Post-warming survival (re-expansion of blastocoele within 24 h) of ICSI-derived fully-expanded blastocysts (80%) was similar to that of their IVF-derived counterparts (88%). However, the ability of ICSI-derived expanding blastocysts to survive vitrification procedures (61%) was lower than that of IVF-derived blastocysts (85%; P < 0.05), although the ICSI- and IVF-derived fresh blastocysts were of similar quality. The age of the blastocysts before vitrification did not affect cryotolerance for either ICSI-derived (73 and 59% for Days 7 and 8 embryos, respectively) or IVF-derived blastocysts (86% for both Days 7 and 8 embryos). At 24 h of post-warming culture, ICSI-derived blastocysts surviving vitrification contained a higher proportion of dead cells than their IVF-derived counterparts (5-13% vs. 2-4%; P < 0.05), but these proportions were not different from those of fresh control embryos. There was an adverse effect of vitrification on the ability of blastocysts to hatch within 72 h of culture only in IVF-derived Day 8 blastocysts (41 and 70% in vitrified and fresh control groups, respectively). In conclusion, the proportion of blastocysts that survived vitrification procedures was similar for ICSI- and IVF-derived bovine blastocysts if the former were cultured to the fully-expanded stage prior to vitrification, with no significant difference between embryos harvested on Day 7 versus Day 8.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0093-691X
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00862972
PubMed
関連識別子
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=20580073
関連名称
関連名称 20580073
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.theriogenology.2010.04.033
関連名称
関連名称 10.1016/j.theriogenology.2010.04.033
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000281941500014
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