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Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age
http://hdl.handle.net/10091/16148
http://hdl.handle.net/10091/16148e41c9e6f-d2b3-45db-baf6-f5ad9bafac94
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||
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公開日 | 2012-12-19 | |||||||||||||||||||
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タイトル | Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age | |||||||||||||||||||
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言語 | eng | |||||||||||||||||||
DOI | ||||||||||||||||||||
関連識別子 | https://doi.org/10.1016/j.theriogenology.2010.04.033 | |||||||||||||||||||
関連名称 | 10.1016/j.theriogenology.2010.04.033 | |||||||||||||||||||
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主題 | Bovine blastocysts, Cryotop, MVC vitrification, IVF, ICSI | |||||||||||||||||||
資源タイプ | ||||||||||||||||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
タイプ | journal article | |||||||||||||||||||
著者 |
Abdalla, H
× Abdalla, H
× Shimoda, M
× Hara, H
× Morita, H
× Kuwayama, M
× Hirabayashi, M
× Hochi, S
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信州大学研究者総覧へのリンク | ||||||||||||||||||||
氏名 | Hochi, S | |||||||||||||||||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html | |||||||||||||||||||
出版者 | ||||||||||||||||||||
出版者 | ELSEVIER SCIENCE INC | |||||||||||||||||||
引用 | ||||||||||||||||||||
内容記述 | THERIOGENOLOGY. 74(6):1028-1035 (2010) | |||||||||||||||||||
書誌情報 |
THERIOGENOLOGY 巻 74, 号 6, p. 1028-1035, 発行日 2010-10 |
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内容記述 | The objective was to investigate the effects of developmental stage (fully-expanded or expanding blastocysts) and/or age (harvested on Days 7 or 8) on post-vitrification in vitro survival of bovine blastocysts derived from intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF). Post-warming survival (re-expansion of blastocoele within 24 h) of ICSI-derived fully-expanded blastocysts (80%) was similar to that of their IVF-derived counterparts (88%). However, the ability of ICSI-derived expanding blastocysts to survive vitrification procedures (61%) was lower than that of IVF-derived blastocysts (85%; P < 0.05), although the ICSI- and IVF-derived fresh blastocysts were of similar quality. The age of the blastocysts before vitrification did not affect cryotolerance for either ICSI-derived (73 and 59% for Days 7 and 8 embryos, respectively) or IVF-derived blastocysts (86% for both Days 7 and 8 embryos). At 24 h of post-warming culture, ICSI-derived blastocysts surviving vitrification contained a higher proportion of dead cells than their IVF-derived counterparts (5-13% vs. 2-4%; P < 0.05), but these proportions were not different from those of fresh control embryos. There was an adverse effect of vitrification on the ability of blastocysts to hatch within 72 h of culture only in IVF-derived Day 8 blastocysts (41 and 70% in vitrified and fresh control groups, respectively). In conclusion, the proportion of blastocysts that survived vitrification procedures was similar for ICSI- and IVF-derived bovine blastocysts if the former were cultured to the fully-expanded stage prior to vitrification, with no significant difference between embryos harvested on Day 7 versus Day 8. | |||||||||||||||||||
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収録物識別子タイプ | ISSN | |||||||||||||||||||
収録物識別子 | 0093-691X | |||||||||||||||||||
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収録物識別子タイプ | NCID | |||||||||||||||||||
収録物識別子 | AA00862972 | |||||||||||||||||||
PubMed | ||||||||||||||||||||
識別子タイプ | PMID | |||||||||||||||||||
関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=20580073 | |||||||||||||||||||
関連名称 | 20580073 | |||||||||||||||||||
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出版タイプ | AM | |||||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||
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URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000281941500014 |
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Cite as
Abdalla, H, Shimoda, M, Hara, H, Morita, H, Kuwayama, M, Hirabayashi, M, Hochi, S, 2010, Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age: ELSEVIER SCIENCE INC, 1028–1035 p.
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