ログイン
言語:

WEKO3

  • トップ
  • コミュニティ
  • ランキング
AND
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "f286f435-ce76-4c43-afcc-def6e262e11b"}, "_deposit": {"id": "13452", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "13452"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00013452", "sets": ["1309:1310"]}, "author_link": ["41001", "41002", "41003", "41004"], "item_1628147817048": {"attribute_name": "\u51fa\u7248\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2001-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "14", "bibliographicPageEnd": "4094", "bibliographicPageStart": "4086", "bibliographicVolumeNumber": "268", "bibliographic_titles": [{"bibliographic_title": "European Journal of Biochemistry"}]}]}, "item_6_description_19": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "The definitive version is available at www.blackwell-synergy.com.", "subitem_description_type": "Other"}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Scopoletin is one of the phytoalexins in tobacco. Cells of the T-13 cell line (Nicotiana tabacum L. Bright Yellow) accumulate a large amount of scopoletin, also known as 7-hydroxy-6-methoxycoumarin, as a glucoconjugate, scopolin, in vacuoles. We report here the molecular cloning of glucosyltransferases that can catalyze the glucosylation of many kinds of secondary metabolites including scopoletin. Two cDNAs encoding glucosyltransferase (NtGT1a and NtGT1b) were isolated from a cDNA library derived from the tobacco T-13 cell line by screening with heterologous cDNAs as a probe. The deduced amino-acid sequences of NtGT1a and NtGT1b  exhibited 92% identity with each other, \u2248 20\u201350% identities with other reported glucosyltransferases. Heterologous expression of these genes in Escherichia coli showed that the recombinant enzymes had glucosylation activity against both flavonoids and coumarins. They also strongly reacted with 2-naphthol as a substrate. These recombinant enzymes can utilize UDP-glucose as the sugar donor, but they can also utilize UDP-xylose as a weak donor. RNA blot analysis showed that these genes are induced by salicylic acid and auxin, but the time course of the expression was different. This result is similar to the changes in scopoletin glucosylation activity in these tobacco cells after addition of these plant growth regulators. These results might suggest that one of the roles of the products of these genes is scopoletin glucosylation, in response to salicylic acid and/or auxin, together with the other glucosyltransferases in tobacco cells.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_31": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\uff1amime\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "European Journal of Biochemistry. 268(14):4086-4094 (2001)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Taguchi, G", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html"}, {"subitem_link_text": "Hayashida, N", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.HVfmjekV.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000170179800025"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "11454003"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=11454003", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1046/j.1432-1327.2001.02325.x"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1046/j.1432-1327.2001.02325.x", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0014-2956", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0014-2956", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11320858", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Nicotiana tabacum L. cv Bright Yellow; glucosyltransferase; phenylpropanoid; 2,4-dichlorophenoxyacetic acid; salicylic acid"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "UDP-GLUCOSE; O-XYLOSYLTRANSFERASE; METHYL JASMONATE; MESSENGER-RNA; PURIFICATION; ACCUMULATION; GENES; 7-O-GLUCOSYLTRANSFERASE; 5-O-GLUCOSYLTRANSFERASE; GLYCOSYLTRANSFERASE"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Scopoletin is one of the phytoalexins in tobacco. Cells of the T-13 cell line (Nicotiana tabacum L. Bright Yellow) accumulate a large amount of scopoletin, also known as 7-hydroxy-6-methoxycoumarin, as a glucoconjugate, scopolin, in vacuoles. We report here the molecular cloning of glucosyltransferases that can catalyze the glucosylation of many kinds of secondary metabolites including scopoletin. Two cDNAs encoding glucosyltransferase (NtGT1a and NtGT1b) were isolated from a cDNA library derived from the tobacco T-13 cell line by screening with heterologous cDNAs as a probe. The deduced amino-acid sequences of NtGT1a and NtGT1b  exhibited 92% identity with each other, \u2248 20\u201350% identities with other reported glucosyltransferases. Heterologous expression of these genes in Escherichia coli showed that the recombinant enzymes had glucosylation activity against both flavonoids and coumarins. They also strongly reacted with 2-naphthol as a substrate. These recombinant enzymes can utilize UDP-glucose as the sugar donor, but they can also utilize UDP-xylose as a weak donor. RNA blot analysis showed that these genes are induced by salicylic acid and auxin, but the time course of the expression was different. This result is similar to the changes in scopoletin glucosylation activity in these tobacco cells after addition of these plant growth regulators. These results might suggest that one of the roles of the products of these genes is scopoletin glucosylation, in response to salicylic acid and/or auxin, together with the other glucosyltransferases in tobacco cells."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Taguchi,  G"}], "nameIdentifiers": [{"nameIdentifier": "41001", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yazawa,  T"}], "nameIdentifiers": [{"nameIdentifier": "41002", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hayashida,  N"}], "nameIdentifiers": [{"nameIdentifier": "41003", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okazaki,  M"}], "nameIdentifiers": [{"nameIdentifier": "41004", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "EJB (2001).pdf", "filesize": [{"value": "6.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 6700000.0, "url": {"label": "EJB (2001).pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/13452/files/EJB (2001).pdf"}, "version_id": "613e80c7-fc70-45bc-8a55-45a2f9b5358e"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Nicotiana tabacum L. cv Bright Yellow", "subitem_subject_scheme": "Other"}, {"subitem_subject": "glucosyltransferase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "phenylpropanoid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "2,4-dichlorophenoxyacetic acid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "salicylic acid", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Molecular cloning and heterologous expression of novel glucosyltransferases from tobacco cultured cells that have broad substrate specificity and are induced by salicylic acid and auxin.", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Molecular cloning and heterologous expression of novel glucosyltransferases from tobacco cultured cells that have broad substrate specificity and are induced by salicylic acid and auxin.", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1309/1310"], "permalink_uri": "http://hdl.handle.net/10091/10115", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2010-05-26"}, "publish_date": "2010-05-26", "publish_status": "0", "recid": "13452", "relation": {}, "relation_version_is_last": true, "title": ["Molecular cloning and heterologous expression of novel glucosyltransferases from tobacco cultured cells that have broad substrate specificity and are induced by salicylic acid and auxin."], "weko_shared_id": -1}
  1. 080 繊維学部
  2. 0801 学術論文

Molecular cloning and heterologous expression of novel glucosyltransferases from tobacco cultured cells that have broad substrate specificity and are induced by salicylic acid and auxin.

http://hdl.handle.net/10091/10115
d739bd42-20b3-4b06-8929-8211b6ebf061
名前 / ファイル ライセンス アクション
EJB EJB (2001).pdf (6.7 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2010-05-26
タイトル
言語 en
タイトル Molecular cloning and heterologous expression of novel glucosyltransferases from tobacco cultured cells that have broad substrate specificity and are induced by salicylic acid and auxin.
言語
言語 eng
キーワード
主題Scheme Other
主題 Nicotiana tabacum L. cv Bright Yellow
キーワード
主題Scheme Other
主題 glucosyltransferase
キーワード
主題Scheme Other
主題 phenylpropanoid
キーワード
主題Scheme Other
主題 2,4-dichlorophenoxyacetic acid
キーワード
主題Scheme Other
主題 salicylic acid
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Taguchi, G

× Taguchi, G

WEKO 41001

Taguchi, G

Search repository
Yazawa, T

× Yazawa, T

WEKO 41002

Yazawa, T

Search repository
Hayashida, N

× Hayashida, N

WEKO 41003

Hayashida, N

Search repository
Okazaki, M

× Okazaki, M

WEKO 41004

Okazaki, M

Search repository
信州大学研究者総覧へのリンク
氏名 Taguchi, G
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html
信州大学研究者総覧へのリンク
氏名 Hayashida, N
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HVfmjekV.html
引用
内容記述タイプ Other
内容記述 European Journal of Biochemistry. 268(14):4086-4094 (2001)
書誌情報 European Journal of Biochemistry

巻 268, 号 14, p. 4086-4094, 発行日 2001-12
内容記述
内容記述タイプ Other
内容記述 The definitive version is available at www.blackwell-synergy.com.
抄録
内容記述タイプ Abstract
内容記述 Scopoletin is one of the phytoalexins in tobacco. Cells of the T-13 cell line (Nicotiana tabacum L. Bright Yellow) accumulate a large amount of scopoletin, also known as 7-hydroxy-6-methoxycoumarin, as a glucoconjugate, scopolin, in vacuoles. We report here the molecular cloning of glucosyltransferases that can catalyze the glucosylation of many kinds of secondary metabolites including scopoletin. Two cDNAs encoding glucosyltransferase (NtGT1a and NtGT1b) were isolated from a cDNA library derived from the tobacco T-13 cell line by screening with heterologous cDNAs as a probe. The deduced amino-acid sequences of NtGT1a and NtGT1b exhibited 92% identity with each other, ≈ 20–50% identities with other reported glucosyltransferases. Heterologous expression of these genes in Escherichia coli showed that the recombinant enzymes had glucosylation activity against both flavonoids and coumarins. They also strongly reacted with 2-naphthol as a substrate. These recombinant enzymes can utilize UDP-glucose as the sugar donor, but they can also utilize UDP-xylose as a weak donor. RNA blot analysis showed that these genes are induced by salicylic acid and auxin, but the time course of the expression was different. This result is similar to the changes in scopoletin glucosylation activity in these tobacco cells after addition of these plant growth regulators. These results might suggest that one of the roles of the products of these genes is scopoletin glucosylation, in response to salicylic acid and/or auxin, together with the other glucosyltransferases in tobacco cells.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0014-2956
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11320858
PubMed
関連識別子
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=11454003
関連名称
関連名称 11454003
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1046/j.1432-1327.2001.02325.x
関連名称
関連名称 10.1046/j.1432-1327.2001.02325.x
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000170179800025
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 11:02:12.460920
Show All versions

Share

Mendeley CiteULike Twitter Facebook Print Addthis

Cite as

Export

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by CERN Data Centre & Invenio


Powered by CERN Data Centre & Invenio