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Molecular cloning and heterologous expression of novel glucosyltransferases from tobacco cultured cells that have broad substrate specificity and are induced by salicylic acid and auxin.
http://hdl.handle.net/10091/10115
http://hdl.handle.net/10091/10115d739bd42-20b3-4b06-8929-8211b6ebf061
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
EJB (2001).pdf (6.7 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-05-26 | |||||
| タイトル | ||||||
| タイトル | Molecular cloning and heterologous expression of novel glucosyltransferases from tobacco cultured cells that have broad substrate specificity and are induced by salicylic acid and auxin. | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1046/j.1432-1327.2001.02325.x | |||||
| 関連名称 | 10.1046/j.1432-1327.2001.02325.x | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Nicotiana tabacum L. cv Bright Yellow | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | glucosyltransferase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | phenylpropanoid | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 2,4-dichlorophenoxyacetic acid | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | salicylic acid | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Taguchi, G
× Taguchi, G× Yazawa, T× Hayashida, N× Okazaki, M |
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| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Taguchi, G | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html | |||||
| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Hayashida, N | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HVfmjekV.html | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | European Journal of Biochemistry. 268(14):4086-4094 (2001) | |||||
| 書誌情報 |
European Journal of Biochemistry 巻 268, 号 14, p. 4086-4094, 発行日 2001-12 |
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| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The definitive version is available at www.blackwell-synergy.com. | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Scopoletin is one of the phytoalexins in tobacco. Cells of the T-13 cell line (Nicotiana tabacum L. Bright Yellow) accumulate a large amount of scopoletin, also known as 7-hydroxy-6-methoxycoumarin, as a glucoconjugate, scopolin, in vacuoles. We report here the molecular cloning of glucosyltransferases that can catalyze the glucosylation of many kinds of secondary metabolites including scopoletin. Two cDNAs encoding glucosyltransferase (NtGT1a and NtGT1b) were isolated from a cDNA library derived from the tobacco T-13 cell line by screening with heterologous cDNAs as a probe. The deduced amino-acid sequences of NtGT1a and NtGT1b exhibited 92% identity with each other, ≈ 20–50% identities with other reported glucosyltransferases. Heterologous expression of these genes in Escherichia coli showed that the recombinant enzymes had glucosylation activity against both flavonoids and coumarins. They also strongly reacted with 2-naphthol as a substrate. These recombinant enzymes can utilize UDP-glucose as the sugar donor, but they can also utilize UDP-xylose as a weak donor. RNA blot analysis showed that these genes are induced by salicylic acid and auxin, but the time course of the expression was different. This result is similar to the changes in scopoletin glucosylation activity in these tobacco cells after addition of these plant growth regulators. These results might suggest that one of the roles of the products of these genes is scopoletin glucosylation, in response to salicylic acid and/or auxin, together with the other glucosyltransferases in tobacco cells. | |||||
| 資源タイプ（コンテンツの種類） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0014-2956 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11320858 | |||||
| PubMed | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=11454003 | |||||
| 関連名称 | 11454003 | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| WoS | ||||||
| 表示名 | Web of Science | |||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000170179800025 | |||||
