Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2010-05-26 |
タイトル |
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タイトル |
Cloning and characterization of a glucosyltransferase that reacts on 7-hydroxyl group of flavonol and 3-hydroxyl group of coumarin from tobacco cells. |
言語 |
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言語 |
eng |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.abb.2003.09.027 |
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関連名称 |
10.1016/j.abb.2003.09.027 |
キーワード |
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主題 |
Nicotiana tabacum L. cv Bright Yellow, Glucosyltransferase, Flavonoid 7-O-glucosyltransferase, 3-Hydroxycoumarin, Kaempferol, Phenolic compound, Plant-hormone-inducible |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Taguchi, G
Ubukata, T
Hayashida, N
Yamamoto, H
Okazaki, M
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信州大学研究者総覧へのリンク |
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氏名 |
Taguchi, G |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html |
信州大学研究者総覧へのリンク |
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氏名 |
Hayashida, N |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.HVfmjekV.html |
出版者 |
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出版者 |
Elsevier |
引用 |
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内容記述 |
Archives of Biochemistry and Biophysics. 420(1):95-102 (2003) |
書誌情報 |
Archives of Biochemistry and Biophysics
巻 420,
号 1,
p. 95-102,
発行日 2003-11
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抄録 |
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内容記述 |
In higher plants, secondary metabolites are often converted to their glycoconjugates by glycosyltransferases (GTases). We cloned a cDNA encoding GTase (NtGT2) from tobacco (Nicotiana tabacum L.). The recombinant enzyme expressed in Escherichia coli (rNTGT2) showed glucosylation activity against several kinds of phenolic compounds, particularly the 7-hydroxyl group of flavonoids and 3-hydroxycoumarin. The Km values of kaempferol and 3-hydroxycoumarin with rNTGT2 are 6.5 μM and 23.6 μM, respectively. The deduced amino acid sequence of NTGT2 shows 60–70% identity to that of anthocyanin 5-O-glucosyltransferase (A5GT); rNTGT2 did not show activity against the anthocyanins tested. NtGT2 gene expression was induced by treating tobacco cells with plant hormones such as salicylic acid. We consider that NtGT2 gene might have evolved from the same ancestral gene as the A5GT genes to the stress-inducible GTases that react on several phenolic compounds. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0003-9861 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00547159 |
他の資源との関係:URI |
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関連識別子 |
http://www.elsevier.com/locate/yabbi |
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関連名称 |
http://www.elsevier.com/locate/yabbi |
PubMed |
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関連識別子 |
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=14622979 |
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関連名称 |
14622979 |
出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
WoS |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000186849500011 |