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  1. 080 繊維学部
  2. 0801 学術論文

Cloning and characterization of a glucosyltransferase that reacts on 7-hydroxyl group of flavonol and 3-hydroxyl group of coumarin from tobacco cells.

http://hdl.handle.net/10091/10114
d2ee4cc9-5054-4050-9d10-6da5ff8ff5ea
名前 / ファイル ライセンス アクション
ABB(2003).pdf ABB(2003).pdf (3.5 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2010-05-26
タイトル
言語 en
タイトル Cloning and characterization of a glucosyltransferase that reacts on 7-hydroxyl group of flavonol and 3-hydroxyl group of coumarin from tobacco cells.
言語
言語 eng
キーワード
主題Scheme Other
主題 Nicotiana tabacum L. cv Bright Yellow
キーワード
主題Scheme Other
主題 Glucosyltransferase
キーワード
主題Scheme Other
主題 Flavonoid 7-O-glucosyltransferase
キーワード
主題Scheme Other
主題 3-Hydroxycoumarin
キーワード
主題Scheme Other
主題 Kaempferol
キーワード
主題Scheme Other
主題 Phenolic compound
キーワード
主題Scheme Other
主題 Plant-hormone-inducible
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Taguchi, G

× Taguchi, G

WEKO 41005

Taguchi, G

Search repository
Ubukata, T

× Ubukata, T

WEKO 41006

Ubukata, T

Search repository
Hayashida, N

× Hayashida, N

WEKO 41007

Hayashida, N

Search repository
Yamamoto, H

× Yamamoto, H

WEKO 41008

Yamamoto, H

Search repository
Okazaki, M

× Okazaki, M

WEKO 41009

Okazaki, M

Search repository
信州大学研究者総覧へのリンク
氏名 Taguchi, G
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html
信州大学研究者総覧へのリンク
氏名 Hayashida, N
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HVfmjekV.html
出版者
出版者 Elsevier
引用
内容記述タイプ Other
内容記述 Archives of Biochemistry and Biophysics. 420(1):95-102 (2003)
書誌情報 Archives of Biochemistry and Biophysics

巻 420, 号 1, p. 95-102, 発行日 2003-11
抄録
内容記述タイプ Abstract
内容記述 In higher plants, secondary metabolites are often converted to their glycoconjugates by glycosyltransferases (GTases). We cloned a cDNA encoding GTase (NtGT2) from tobacco (Nicotiana tabacum L.). The recombinant enzyme expressed in Escherichia coli (rNTGT2) showed glucosylation activity against several kinds of phenolic compounds, particularly the 7-hydroxyl group of flavonoids and 3-hydroxycoumarin. The Km values of kaempferol and 3-hydroxycoumarin with rNTGT2 are 6.5 μM and 23.6 μM, respectively. The deduced amino acid sequence of NTGT2 shows 60–70% identity to that of anthocyanin 5-O-glucosyltransferase (A5GT); rNTGT2 did not show activity against the anthocyanins tested. NtGT2 gene expression was induced by treating tobacco cells with plant hormones such as salicylic acid. We consider that NtGT2 gene might have evolved from the same ancestral gene as the A5GT genes to the stress-inducible GTases that react on several phenolic compounds.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0003-9861
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00547159
他の資源との関係:URI
関連識別子
識別子タイプ URI
関連識別子 http://www.elsevier.com/locate/yabbi
関連名称
関連名称 http://www.elsevier.com/locate/yabbi
PubMed
関連識別子
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=14622979
関連名称
関連名称 14622979
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.abb.2003.09.027
関連名称
関連名称 10.1016/j.abb.2003.09.027
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000186849500011
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