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When 250 \u03bcM of 1-naphthol  or 2-naphthol was added to the tobacco cells, most of the naphthol was accumulated in the cell as glucosides and in further modified forms. The glucosylation activities against naphthols were increased in proportion to the concentration of naphthols in the culture medium. Addition of 1 mM naphthols caused cell death. Three glucosyltransferase genes, namely NtGT1a, NtGT1b and NtGT3 were isolated and characterized. The recombinant enzymes encoded by these genes showed glucosylation activity against naphthols and other phenolic compounds. It was also shown that these genes were induced following the addition of naphthols to the tobacco cells. 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In this work, detoxification of the exogenously added harmful compound naphthol in tobacco cells (Nicotiana tabacum L. Bright Yellow) was studied. When 250 muM of 1-naphthol or 2-naphthol was added to the tobacco cells, most of the naphthol was accumulated in the cell as glucosides and in further modified forms. The glucosylation activities against naphthols were increased in proportion to the concentration of naphthols in the culture medium. Addition of I mM naphthols caused cell death. Three glucosyltransferase genes, namely NtGT1a, NtGT1b and NtGT3 were isolated and characterized. The recombinant enzymes encoded by these genes showed glucosylation activity against naphthols and other phenolic compounds. It was also shown that these genes were induced following the addition of naphthols to the tobacco cells. These results suggest that naphthols are metabolized by glucosyltransferases whose production is inducible by naphthol itself."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Taguchi,  G"}], "nameIdentifiers": [{"nameIdentifier": "41010", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakamura,  M"}], "nameIdentifiers": [{"nameIdentifier": "41011", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hayashida,  N"}], "nameIdentifiers": [{"nameIdentifier": "41012", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okazaki,  M"}], "nameIdentifiers": [{"nameIdentifier": "41013", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Plant Sci 2003.pdf", "filesize": [{"value": "2.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2000000.0, "url": {"label": "Plant Sci 2003.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/13454/files/Plant Sci 2003.pdf"}, "version_id": "e6ebedfc-725d-4d25-8df4-83febe4727f3"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Nicotiana tabacum L. cv Bright Yellow", "subitem_subject_scheme": "Other"}, {"subitem_subject": "glucosyltransferase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "naphthol", "subitem_subject_scheme": "Other"}, {"subitem_subject": "xenobiotics", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Exogenously added naphthols induce three glucosyltransferases, and are accumulated as glucosides in tobacco cells", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Exogenously added naphthols induce three glucosyltransferases, and are accumulated as glucosides in tobacco cells"}]}, "item_type_id": "6", "owner": "1", "path": ["1309/1310"], "permalink_uri": "http://hdl.handle.net/10091/10112", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2010-05-26"}, "publish_date": "2010-05-26", "publish_status": "0", "recid": "13454", "relation": {}, "relation_version_is_last": true, "title": ["Exogenously added naphthols induce three glucosyltransferases, and are accumulated as glucosides in tobacco cells"], "weko_shared_id": null}