Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2010-01-27 |
タイトル |
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タイトル |
Construction of a Practical SCAR Marker Linked to Clubroot Resistance in Chinese Cabbage, with Intensive Analysis of HC352b Genes |
言語 |
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言語 |
eng |
DOI |
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関連識別子 |
https://doi.org/10.2503/jjshs1.77.150 |
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関連名称 |
10.2503/jjshs1.77.150 |
キーワード |
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主題 |
clubroot resistance, HC352, paralog, sequence characterized amplified region (SCAR), CRa |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Hayashida, N
Takabatake, Y
Nakazawa, N
Aruga, D
Nakanishi, H
Taguchi, G
Sakamoto, K
Matsumoto, E
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信州大学研究者総覧へのリンク |
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氏名 |
Hayashida, N |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.HVfmjekV.html |
信州大学研究者総覧へのリンク |
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氏名 |
Taguchi, G |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html |
出版者 |
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出版者 |
園芸学会 |
引用 |
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内容記述 |
Journal of the Japanese Society for Horticultural Science. 77(2):150-154 (2008) |
書誌情報 |
Journal of the Japanese Society for Horticultural Science
巻 77,
号 2,
p. 150-154,
発行日 2008-04
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抄録 |
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内容記述 |
A restriction fragment length polymorphism (RFLP) marker, HC352b, has identified as closely linked to the CRa locus responsible for clubroot resistance (CR) in a study dissecting CR loci in Chinese cabbage (Brassica rapa L. ssp pekinensis). Unfortunately, the RFLP pattern, including HC352b detected by the cDNA clone HC352, was complicated and confused in its interpretation because it represented multiple-copy loci. To provide a practical HC352b marker for CR breeding programs, a sequence characterized amplified region (SCAR) marker was constructed along the analysis of HC352 genes, followed by its evaluation for CR selection. Schematic characterization of signals was achieved by identification of HC352 homologous genes with their restriction sites, employing CRa-positive and -negative doubled haploid (DH) lines. Genomic sequence information from a set of HC352 homologous genes was analyzed to identify the CRa linked paralog HC352b, followed by the successful designation of a SCAR marker. This correctly predicted the CR phenotypes of all tested individuals of F2 and back-crossed progenies. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1882-3351 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA12177046 |
他の資源との関係:URI |
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関連識別子 |
http://www.jstage.jst.go.jp/ |
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関連名称 |
http://www.jstage.jst.go.jp/ |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
WoS |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&KeyUT=000255101200007&DestApp=WOS |