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Cauda epididymal spermatozoa from Crlj:Wistar rats were frozen in liquid nitrogen (LN2), first dried for 14 hr at 0.37 hPa and then for 3 hr at 0.001 hPa. The dried spermatozoa were stored for 1 year in a desiccator at +25 degrees C, or in a refrigerator at +4 degrees C, or in LN2 at -196 degrees C. Controls consisted of sperm that had only been frozen and stored in LN2. After being stored, spermatozoa were sonicated to dissociate the sperm tail and were injected into oocytes from superovulated Slc:SD rats. The respective fertilization rates of oocytes injected with frozen sperm, or with freeze-dried sperm stored at +25, +4, and -196 degrees C were 79%, 75%, 70%, and 73%. However, the corresponding cleavage rates of injected oocytes were 63%, 1%, 38%, and 36%. After transfer of \u003e80 zygotes of each group into recipients, the respective percentages of full-term normal offspring resulting from frozen sperm or from freeze-dried sperm stored at +25, +4, and -196 degrees C were 36%, 0%, 7%, and 14%. These results demonstrate that the storage temperature significantly influenced the likelihood of term development of rats produced by injection of oocytes with freeze-dried spermatozoa. Chromosomal analysis of the rat spermatozoa in the ICSI oocytes indicated that chromosomal aberration in freeze-dried spermatozoa stored at +25 degrees C (100%) occurred more frequently than in frozen control spermatozoa (41%) and freeze-dried spermatozoa stored at -196 degrees C (35%), and the frequency of chromosomal aberrations in freeze-dried spermatozoa stored at +4 degrees C (65%) was the intermediate. In conclusion, rat spermatozoa freeze-dried and stored at +4 degrees C for 1 year are capable of participating in full-term development after ICSI."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hochi, S"}], "nameIdentifiers": [{"nameIdentifier": "41362", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe, K"}], "nameIdentifiers": [{"nameIdentifier": "41363", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kato, M"}], "nameIdentifiers": [{"nameIdentifier": "41364", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirabayashi, M"}], "nameIdentifiers": [{"nameIdentifier": "41365", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "MRD75(5)890-4(\u002708).pdf", "filesize": [{"value": "155.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 155200.0, "url": {"label": "MRD75(5)890-4(\u002708).pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/13528/files/MRD75(5)890-4(\u002708).pdf"}, "version_id": "ad3f38c8-36a7-4a08-bc58-65046ecc10b4"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Live rats resulting from injection of oocytes with spermatozoa freeze-dried and stored for one year", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Live rats resulting from injection of oocytes with spermatozoa freeze-dried and stored for one year", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1310"], "permalink_uri": "http://hdl.handle.net/10091/1094", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-06-26"}, "publish_date": "2008-06-26", "publish_status": "0", "recid": "13528", "relation": {}, "relation_version_is_last": true, "title": ["Live rats resulting from injection of oocytes with spermatozoa freeze-dried and stored for one year"], "weko_shared_id": -1}
Live rats resulting from injection of oocytes with spermatozoa freeze-dried and stored for one year
http://hdl.handle.net/10091/1094
http://hdl.handle.net/10091/1094c07e9bce-d462-4304-81c3-063bd1a7e046
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-06-26 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Live rats resulting from injection of oocytes with spermatozoa freeze-dried and stored for one year | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Hochi, S
× Hochi, S× Watanabe, K× Kato, M× Hirabayashi, M |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Hochi, S | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html | |||||
出版者 | ||||||
出版者 | WILEY-LISS | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Molecular Reproduction and Development. 75(5): 890-894 (2008) | |||||
書誌情報 |
MOLECULAR REPRODUCTION AND DEVELOPMENT 巻 75, 号 5, p. 890-894, 発行日 2008-05 |
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資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1040-452X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10735246 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1002/mrd.20825 | |||||
関連名称 | 10.1002/mrd.20825 | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000254452000022 |