{"_buckets": {"deposit": "bcec6c09-27fb-4597-97f2-6389d275db5c"}, "_deposit": {"id": "13557", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "13557"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00013557"}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-02", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "51", "bibliographicPageStart": "45", "bibliographicVolumeNumber": "14", "bibliographic_titles": [{"bibliographic_title": "Zygote"}]}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_31": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\uff1amime\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "ZYGOTE. 14(1): 45-51 (2006)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Hochi, S", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000236477700007"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "CAMBRIDGE UNIV PRESS"}]}, "item_6_relation_46": {"attribute_name": "\u4ed6\u306e\u8cc7\u6e90\u3068\u306e\u95a2\u4fc2\uff1aURI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "http://journals.cambridge.org/action/displayJournal?jid=ZYG"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://journals.cambridge.org/action/displayJournal?jid=ZYG", "subitem_relation_type_select": "URI"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1017/S0967199406003522"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.1017/S0967199406003522", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0967-1994", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0967-1994", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10958262", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "interspecies ICSI; minke whale; microtubule-organizing centre (MTOC); sperm aster"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "INTRACYTOPLASMIC SPERM INJECTION; MOUSE OOCYTES; ASTER FORMATION; BOVINE OOCYTES; ACUTOROSTRATA SPERMATOZOA; CHROMATIN CONFIGURATIONS; RAT SPERMATOZOA; HAMSTER OOCYTES; FERTILIZATION; EGGS"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Using an interspecies microinsemination assay with bovine oocytes, it was examined whether centrosomes of Antarctic minke whale spermatozoa function as the microtubule-organizing centre (MTOC). Bull and rat spermatozoa were used as positive and negative controls, respectively. Vitrified-warmed bovine mature oocytes were subjected to immunostaining against alpha-tubulin 4-6 h after intracytoplasmic injection (ICSI) of 5 mM dithiothreitol-treated spermatozoa. Aster formation occurred from whale spermatozoa (33%) and bull spermatozoa (33%), but very little from rat spermatozoa (3%). Activation treatment for the microinseminated oocytes with 7% ethanol + 2 mM 6-dimethylaminopurine resulted in a similar proportion of oocytes forming a whale sperm aster (35% vs 27% in the non-treated group; 4 h after ICSI) but a significantly larger aster (ratio of aster diameter to oocyte diameter, 0.57 vs 0.30 in the non-treated group). These results indicate that the centrosome introduced into bovine oocytes by whale spermatozoa contributes to the MTOC and that assembly of the microtubule network is promoted by oocyte activation."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kobayashi,  T"}], "nameIdentifiers": [{"nameIdentifier": "41490", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Amemiya,  K"}], "nameIdentifiers": [{"nameIdentifier": "41491", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takeuchi,  K"}], "nameIdentifiers": [{"nameIdentifier": "41492", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsujioka,  T"}], "nameIdentifiers": [{"nameIdentifier": "41493", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tominaga,  K"}], "nameIdentifiers": [{"nameIdentifier": "41494", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirabayashi,  M"}], "nameIdentifiers": [{"nameIdentifier": "41495", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishikawa,  H"}], "nameIdentifiers": [{"nameIdentifier": "41496", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fukui,  Y"}], "nameIdentifiers": [{"nameIdentifier": "41497", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hochi,  S"}], "nameIdentifiers": [{"nameIdentifier": "41498", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ZYG14(1)45-51(\u002706).pdf", "filesize": [{"value": "114.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 114800.0, "url": {"label": "ZYG14(1)45-51(\u002706).pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/13557/files/ZYG14(1)45-51(\u002706).pdf"}, "version_id": "3da8be68-ad6e-4b64-afb7-2591b6b83ff9"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Contribution of spermatozoal centrosomes to the microtubiale-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis)", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Contribution of spermatozoal centrosomes to the microtubiale-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis)"}]}, "item_type_id": "6", "owner": "1", "path": ["1309/1310"], "permalink_uri": "http://hdl.handle.net/10091/271", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2007-08-28"}, "publish_date": "2007-08-28", "publish_status": "0", "recid": "13557", "relation": {}, "relation_version_is_last": true, "title": ["Contribution of spermatozoal centrosomes to the microtubiale-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis)"], "weko_shared_id": null}