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Bull and rat spermatozoa were used as positive and negative controls, respectively. Vitrified-warmed bovine mature oocytes were subjected to immunostaining against alpha-tubulin 4-6 h after intracytoplasmic injection (ICSI) of 5 mM dithiothreitol-treated spermatozoa. Aster formation occurred from whale spermatozoa (33%) and bull spermatozoa (33%), but very little from rat spermatozoa (3%). Activation treatment for the microinseminated oocytes with 7% ethanol + 2 mM 6-dimethylaminopurine resulted in a similar proportion of oocytes forming a whale sperm aster (35% vs 27% in the non-treated group; 4 h after ICSI) but a significantly larger aster (ratio of aster diameter to oocyte diameter, 0.57 vs 0.30 in the non-treated group). These results indicate that the centrosome introduced into bovine oocytes by whale spermatozoa contributes to the MTOC and that assembly of the microtubule network is promoted by oocyte activation."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kobayashi, T"}], "nameIdentifiers": [{"nameIdentifier": "41490", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Amemiya, K"}], "nameIdentifiers": [{"nameIdentifier": "41491", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takeuchi, K"}], "nameIdentifiers": [{"nameIdentifier": "41492", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsujioka, T"}], "nameIdentifiers": [{"nameIdentifier": "41493", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tominaga, K"}], "nameIdentifiers": [{"nameIdentifier": "41494", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirabayashi, M"}], "nameIdentifiers": [{"nameIdentifier": "41495", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishikawa, H"}], "nameIdentifiers": [{"nameIdentifier": "41496", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fukui, Y"}], "nameIdentifiers": [{"nameIdentifier": "41497", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hochi, S"}], "nameIdentifiers": [{"nameIdentifier": "41498", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ZYG14(1)45-51(\u002706).pdf", "filesize": [{"value": "114.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 114800.0, "url": {"label": "ZYG14(1)45-51(\u002706).pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/13557/files/ZYG14(1)45-51(\u002706).pdf"}, "version_id": "3da8be68-ad6e-4b64-afb7-2591b6b83ff9"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Contribution of spermatozoal centrosomes to the microtubiale-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis)", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Contribution of spermatozoal centrosomes to the microtubiale-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis)", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1310"], "permalink_uri": "http://hdl.handle.net/10091/271", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-08-28"}, "publish_date": "2007-08-28", "publish_status": "0", "recid": "13557", "relation": {}, "relation_version_is_last": true, "title": ["Contribution of spermatozoal centrosomes to the microtubiale-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis)"], "weko_shared_id": -1}
Contribution of spermatozoal centrosomes to the microtubiale-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis)
http://hdl.handle.net/10091/271
http://hdl.handle.net/10091/27151ede3ed-20de-4a5b-87d7-d2bf29a75ad3
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-08-28 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Contribution of spermatozoal centrosomes to the microtubiale-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis) | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Kobayashi, T
× Kobayashi, T× Amemiya, K× Takeuchi, K× Tsujioka, T× Tominaga, K× Hirabayashi, M× Ishikawa, H× Fukui, Y× Hochi, S |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Hochi, S | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html | |||||
出版者 | ||||||
出版者 | CAMBRIDGE UNIV PRESS | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | ZYGOTE. 14(1): 45-51 (2006) | |||||
書誌情報 |
Zygote 巻 14, 号 1, p. 45-51, 発行日 2006-02 |
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資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0967-1994 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10958262 | |||||
他の資源との関係:URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://journals.cambridge.org/action/displayJournal?jid=ZYG | |||||
関連名称 | http://journals.cambridge.org/action/displayJournal?jid=ZYG | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1017/S0967199406003522 | |||||
関連名称 | 10.1017/S0967199406003522 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000236477700007 |