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Analysis of the genomic structure of fv-hyd1 revealed an open reading frame (ORF) composed of 363 nucleotides and interrupted by three introns. The deduced amino acid sequence of FV-HYD1 showed a similarity to those of other fungal class I hydrophobins and contained eight cysteine residues highly conserved among hydrophobin proteins. The pattern of the hydropathy plot of FV-HYD1 was similar to those of class I hydrophobins. Southern blot analysis of genomic DNA showed that fv-hyd1 existed as a single copy. Northern blot analysis indicated that the fv-hyd1 transcript was not present in vegetative mycelia but markedly increased in level at the primordial stage. Moreover, the fv-hyd1 transcript was abundant even at the mature fruiting body stage. This result indicates that fv-hyd1 could encode a hydrophobin closely associated with fruiting body development."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yamada,  M"}], "nameIdentifiers": [{"nameIdentifier": "41596", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakuraba,  S"}], "nameIdentifiers": [{"nameIdentifier": "41597", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shibata,  K"}], "nameIdentifiers": [{"nameIdentifier": "41598", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Inatomi,  S"}], "nameIdentifiers": [{"nameIdentifier": "41599", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okazaki,  M"}], "nameIdentifiers": [{"nameIdentifier": "41600", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimosaka,  M"}], "nameIdentifiers": [{"nameIdentifier": "41601", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "AMB.pdf", "filesize": [{"value": "1.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 1600000.0, "url": {"label": "AMB.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/13573/files/AMB.pdf"}, "version_id": "c5d35464-19eb-4694-ba42-bb13ae35de56"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Cloning and characterization of a gene coding for a hydrophobin, Fv-hyd1, specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Cloning and characterization of a gene coding for a hydrophobin, Fv-hyd1, specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1309/1310"], "permalink_uri": "http://hdl.handle.net/10091/213", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-05-10"}, "publish_date": "2007-05-10", "publish_status": "0", "recid": "13573", "relation": {}, "relation_version_is_last": true, "title": ["Cloning and characterization of a gene coding for a hydrophobin, Fv-hyd1, specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes"], "weko_shared_id": -1}