{"_buckets": {"deposit": "d4ff6464-5721-45ab-92ef-6ef5d3493e5b"}, "_deposit": {"id": "13580", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "13580"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00013580", "sets": ["1310"]}, "author_link": ["41630", "41631", "41632", "41633"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-05-23", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "400", "bibliographicPageStart": "394", "bibliographicVolumeNumber": "5", "bibliographic_titles": [{"bibliographic_title": "MACROMOLECULAR BIOSCIENCE"}]}]}, "item_6_description_19": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "This is a preprint of an article published in [Fujii, T; Ogiwara, D; Ohkawa, K; Yamamoto, H.,Alkaline phosphatase encapsulated in gellan-chitosan hybrid capsules,MACROMOLECULAR BIOSCIENCE,Vol 5,394-400(2005)]", "subitem_description_type": "Other"}]}, "item_6_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_31": {"attribute_name": "フォーマット：mimeタイプ", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "MACROMOLECULAR BIOSCIENCE. 5(5): 394-400 (2005)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "Fujii, T", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.WVyFjekV.html"}, {"subitem_link_text": "Ohkawa, K", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.gUfejekV.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000229423100004"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "WILEY-V C H VERLAG GMBH"}]}, "item_6_relation_46": {"attribute_name": "他の資源との関係：URI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "http://www3.interscience.wiley.com/cgi-bin/jhome/77002860"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www3.interscience.wiley.com/cgi-bin/jhome/77002860", "subitem_relation_type_select": "URI"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1002/mabi.200400190"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1002/mabi.200400190", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1616-5187", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1616-5187", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11586345", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "biological applications of polymers; encapsulation; enzymes; gellan-chitosan; polyion complex capsule"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "POLYION COMPLEX FIBER; SOLUTION INTERFACES; MICROCAPSULES; PROTEINS; ENZYME; GLASSES; FILMS"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Alkaline phosphatase (ALP) was encapsulated in gellan-chitosan polyion complex (PIC) capsules using a convenient procedure. The recovery of ALP was about 50% when the capsules were prepared by dropping a solution of ALP and gellan mixture (ALP/gellan) into a chitosan solution. When p-nitrophenyl phosphate (p-NPP) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP) were incubated with ALP/gellan-chitosan capsules as substrates for ALP, the transparent colorless capsules changed to yellow and blue, respectively. The encapsulation of ALP into the PIC capsules was also confirmed by SDS-PAGE and immunoblot analyses. The ALP and polypeptides of more than 30 kDa remained without release even after incubation at 4 degrees C for 14 d. The biochemical properties of the encapsulated ALP activity were similar to those of the intact enzyme. When the solution containing p-NPP was loaded on a column packed with ALP/gellan-chitosan capsules at 27 degrees C, approximately 75% of p-NPP was hydrolyzed by passing through the column. No significant leakage of ALP was observed during the procedure, indicating that the capsules were resistant to pressure in the chromatographic operation. Furthermore, 70% of the hydrolytic activity of the packed capsules remained after storage at 4 degrees C for one month. These results suggest that the polyion complex capsules could be useful materials for protein fixation without chemical modification."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Fujii,  T"}], "nameIdentifiers": [{"nameIdentifier": "41630", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ogiwara,  D"}], "nameIdentifiers": [{"nameIdentifier": "41631", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohkawa,  K"}], "nameIdentifiers": [{"nameIdentifier": "41632", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamamoto,  H"}], "nameIdentifiers": [{"nameIdentifier": "41633", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Gellan-Chitosan.pdf", "filesize": [{"value": "601.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 601200.0, "url": {"label": "Gellan-Chitosan.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/13580/files/Gellan-Chitosan.pdf"}, "version_id": "7650aaaf-8d5d-4aa4-b025-670d0d4e839e"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Alkaline phosphatase encapsulated in gellan-chitosan hybrid capsules", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Alkaline phosphatase encapsulated in gellan-chitosan hybrid capsules", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1310"], "permalink_uri": "http://hdl.handle.net/10091/177", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-04-20"}, "publish_date": "2007-04-20", "publish_status": "0", "recid": "13580", "relation": {}, "relation_version_is_last": true, "title": ["Alkaline phosphatase encapsulated in gellan-chitosan hybrid capsules"], "weko_shared_id": -1}