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  1. 080 繊維学部
  2. 0802 紀要・刊行物
  3. 08030 先端繊維技術科学に関する研究報告
  4. Vol. 11

15-2-8 : キチン・キトサンの酵素変換による高機能化に関する研究

http://hdl.handle.net/10091/13457
a42ee192-062d-4e7b-8786-fed59d38ce76
名前 / ファイル ライセンス アクション
AFSTT11-210.pdf AFSTT11-210.pdf (889.2 kB)
Item type 研究報告書 / Research Paper(1)
公開日 2011-12-05
タイトル
言語 ja
タイトル 15-2-8 : キチン・キトサンの酵素変換による高機能化に関する研究
言語
言語 jpn
資源タイプ
資源 http://purl.org/coar/resource_type/c_18ws
タイプ research report
その他(別言語等)のタイトル
その他のタイトル 天然繊維の高機能化と応用
著者 下坂, 誠

× 下坂, 誠

WEKO 43582

下坂, 誠

Search repository
小平, 律子

× 小平, 律子

WEKO 43583

小平, 律子

Search repository
信州大学研究者総覧へのリンク
氏名 下坂, 誠
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html
出版者
出版者 信州大学繊維学部
引用
内容記述タイプ Other
内容記述 先進ファイバー工学研究教育拠点研究成果報告書 11: 51-52(2005)
書誌情報 先進ファイバー工学研究教育拠点研究成果報告書

巻 11, p. 51-52, 発行日 2005-03-31
抄録
内容記述タイプ Abstract
内容記述 A gene (csnC) Coding for chitosanase secreted in medium was isolated from the koji mold Aspergillus oryzae. CsnC ORF contained serine- and threonine-rich linker and three repeated sequences in its C-terminal, which were not observed in other fungal chitosanases. Tne cDNA corresponding to mature chitosanase was synthesized by a reverse transcriptase-PCR, and introduced into cells of the yeast Pichia pastoris by using an expression vector The resulting transformant secreted active recombinant chitosanase. A protein lacking the repeated sequence (CsnCΔR3) was also expressed and used for kinetic analysis. The repeated sequence was found to be involved in binding of the enzyme to insoluble substrate chitosan. Environmental DNA was prepared from microorganisms in soil amended with chitin. A part of 16S ribosomal DNA was PCR-amplified by using the environmental DNA as a template. The resulting DNA fragments were fractionated by a denaturant gradient gel electrophoresis (DGGE). The intensities of several DNA bands increased significantly after addition of chitin. Determination of nucleotide sequence of these DNA fragments indicated that most of them showed a similarity, to 16S rDNA sequences derived from unculturable bacteria deposited in the database.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA1200368X
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
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