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15-2-14 :細胞壁合成・修飾・分解機構の研究
http://hdl.handle.net/10091/13635
http://hdl.handle.net/10091/13635686ce37c-d642-4857-8cdb-b77a2b6b486b
名前 / ファイル | ライセンス | アクション |
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Item type | 研究報告書 / Research Paper(1) | |||||
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公開日 | 2012-02-23 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 15-2-14 :細胞壁合成・修飾・分解機構の研究 | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_18ws | |||||
タイプ | research report | |||||
その他(別言語等)のタイトル | ||||||
その他のタイトル | バイオファイバー生合成機構の解明 | |||||
著者 |
関口, 順一
× 関口, 順一× 山本, 博規 |
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信州大学研究者総覧へのリンク | ||||||
氏名 | 山本, 博規 | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uhDNjekV.html | |||||
出版者 | ||||||
出版者 | 信州大学繊維学部 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 先進ファイバー工学研究教育拠点研究成果報告書 10: 60-60(2004) | |||||
書誌情報 |
先進ファイバー工学研究教育拠点研究成果報告書 巻 10, p. 60-60, 発行日 2004-03-31 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Modification of peptidoglycan is important in various bacteria. Especially Bacillus subtilis produces spore under the starvation conditions. We investigated the modification of spore peptidoglycan called cortex with the use of the polysaccharide deacetylase gene (pdaA) of Bacillus subtilis. PdaA-deficient spore did not lead to germination and this mutant completely lacked muramic-delta-lactam which is essential to germination. In-vitro experiments indicated that PdaA did not catalyze deacetylation reaction when the native polysaccharide was used as a substrate, but catalyzed deacetylation when the amidase-digested polysaccharide was used as a substrate. These results indicate PdaA plays a key role in cortex biosynthesis. On the other hand, cell surface targeting of peptidoglycan hydrolases was investigated by the immuno-fluorescence microscopy with 3xFLAG tagged protein, and localization of three proteins was identified. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1200368X | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |