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This method has remained practically unchanged for more than 20 years, but the roles these molecules play are not definitively understood. To gain a better understanding, we have compared PEG transformation to a protocol using polyethylenimine (PEI) that is the basis for non-viral transfection in mammals and which has a well established molecular model for assisting DNA uptake. Protoplasts of Aspergillus nidulans could be transformed in the presence of Ca2+ with a relatively high ratio of PEI to DNA molecules. By comparing PEI and PEG in terms of interaction with DNA, fungal protoplasts, and response to different transformation conditions, we propose that the role of PEG is most likely to function after transforming DNA is incorporated into protoplasts, rather than the accepted view that it functions outside of the cell. 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  1. 100 全学教育機構
  2. 1001 学術論文

Comparison between polyethylene glycol- and polyethylenimine-mediated transformation of Aspergillus nidulans

http://hdl.handle.net/10091/1153
bf6594ef-566b-47dd-b287-16a89167e11a
名前 / ファイル ライセンス アクション
Kuwanoetalrevision[1].pdf Kuwanoetalrevision[1].pdf (544.6 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-09-11
タイトル
言語 en
タイトル Comparison between polyethylene glycol- and polyethylenimine-mediated transformation of Aspergillus nidulans
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Kuwano, T

× Kuwano, T

WEKO 46004

Kuwano, T

Search repository
Shirataki, C

× Shirataki, C

WEKO 46005

Shirataki, C

Search repository
Itoh, Y

× Itoh, Y

WEKO 46006

Itoh, Y

Search repository
信州大学研究者総覧へのリンク
氏名 Itoh, Y
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.OVTNOUkh.html
出版者
出版者 SPRINGER
引用
内容記述タイプ Other
内容記述 CURRENT GENETICS. 54(2):95-103(2008)
書誌情報 CURRENT GENETICS

巻 54, 号 2, p. 95-103, 発行日 2008-07
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0172-8083
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00146001
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1007/s00294-008-0204-z
関連名称
関連名称 10.1007/s00294-008-0204-z
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000258152800004
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