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GlcNAc oligomers prepared from chitin have useful biological activities, such as immunostimulation and the induction of plant defence responses. Microbial chitinolytic enzymes have been investigated extensively for their potential use in the eco-friendly enzymatic production of GlcNAc and its oligomers. Chitiniphilus shinanonensis SAY3(T) is a recently found bacterium with a strong chitinolytic activity. The chitinolytic enzymes from this strain are potentially useful for the efficient production of GlcNAc and its oligomers from chitin. ChiL from C. shinanonensis is an endo-type chitinase belonging to the family 18 glycoside hydrolases (GH18). To understand the enzymatic reaction mechanism of ChiL and utilize it for further enzyme engineering, the catalytic domain (41406) of ChiL, the construct for which was carefully designed, was expressed, purified and crystallized by the vapour-diffusion method. The crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 69.19, b = 81.55, c = 130.01 angstrom, and diffracted to 1.25 angstrom resolution. The Matthews coefficient (VM = 2.2 angstrom 3 Da(-1)) suggested the presence of two monomers per asymmetric unit with a solvent content of 45%."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ueda, Miruku"}], "nameIdentifiers": [{"nameIdentifier": "49718", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimosaka, Makoto"}], "nameIdentifiers": [{"nameIdentifier": "49719", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Arai, Ryoichi"}], "nameIdentifiers": [{"nameIdentifier": "49720", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-12-16"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ChiL_Cryst_ActaF2015.pdf", "filesize": [{"value": "370.8 kB"}], "format": "binary/octet-stream", "future_date_message": "", "is_thumbnail": false, "licensefree": "Copyright © International Union of Crystallography", "licensetype": "license_note", "mimetype": "binary/octet-stream", "size": 370800.0, "url": {"label": "ChiL_Cryst_ActaF2015", "url": "https://soar-ir.repo.nii.ac.jp/record/17805/files/ChiL_Cryst_ActaF2015.pdf"}, "version_id": "181a136d-4898-455a-8bf3-04454032c3f2"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-12-16"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "ChiL_Cryst_ActaF2015_SupInfo.pdf", "filesize": [{"value": "1.8 MB"}], "format": "binary/octet-stream", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "binary/octet-stream", "size": 1800000.0, "url": {"label": "ChiL_Cryst_ActaF2015_SupInfo", "url": "https://soar-ir.repo.nii.ac.jp/record/17805/files/ChiL_Cryst_ActaF2015_SupInfo.pdf"}, "version_id": "3d8afd51-1efb-441f-9e91-51152d0b07dd"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "chitinase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "chitinolytic enzyme", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Chitiniphilus shinanonensis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "chitin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "N-acetylglucosamine", "subitem_subject_scheme": "Other"}, {"subitem_subject": "family 18 glycoside hydrolases", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1310"], "permalink_uri": "http://hdl.handle.net/10091/00018574", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2015-12-16"}, "publish_date": "2015-12-16", "publish_status": "0", "recid": "17805", "relation": {}, "relation_version_is_last": true, "title": ["Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis"], "weko_shared_id": -1}
Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis
http://hdl.handle.net/10091/00018574
http://hdl.handle.net/10091/0001857430341ab7-79b5-489b-954c-94be37b60344
名前 / ファイル | ライセンス | アクション |
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ChiL_Cryst_ActaF2015 (370.8 kB)
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ChiL_Cryst_ActaF2015_SupInfo (1.8 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-12-16 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis | |||||
言語 | ||||||
言語 | eng | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1107/S2053230X15022001 | |||||
関連名称 | 10.1107/S2053230X15022001 | |||||
キーワード | ||||||
主題 | chitinase, chitinolytic enzyme, Chitiniphilus shinanonensis, chitin, N-acetylglucosamine, family 18 glycoside hydrolases | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Ueda, Miruku
× Ueda, Miruku× Shimosaka, Makoto× Arai, Ryoichi |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Shimosaka, Makoto | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Arai, Ryoichi | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OVkUjFkV.html | |||||
出版者 | ||||||
出版者 | International Union of Crystallography | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta Crystallographica Section F. F7(12):1516-1520 (2015) | |||||
書誌情報 |
Acta Crystallographica Section F 巻 F71, 号 12, p. 1516-1520, 発行日 2015-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2053-230X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12097708 | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=26625294 | |||||
関連名称 | 26625294 | |||||
権利 | ||||||
権利情報 | Copyright © International Union of Crystallography | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000369376500011 |