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GlcNAc oligomers prepared from chitin have useful biological activities, such as immunostimulation and the induction of plant defence responses. Microbial chitinolytic enzymes have been investigated extensively for their potential use in the eco-friendly enzymatic production of GlcNAc and its oligomers. Chitiniphilus shinanonensis SAY3(T) is a recently found bacterium with a strong chitinolytic activity. The chitinolytic enzymes from this strain are potentially useful for the efficient production of GlcNAc and its oligomers from chitin. ChiL from C. shinanonensis is an endo-type chitinase belonging to the family 18 glycoside hydrolases (GH18). To understand the enzymatic reaction mechanism of ChiL and utilize it for further enzyme engineering, the catalytic domain (41406) of ChiL, the construct for which was carefully designed, was expressed, purified and crystallized by the vapour-diffusion method. The crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 69.19, b = 81.55, c = 130.01 angstrom, and diffracted to 1.25 angstrom resolution. 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  1. 080 繊維学部
  2. 0801 学術論文

Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis

http://hdl.handle.net/10091/00018574
http://hdl.handle.net/10091/00018574
30341ab7-79b5-489b-954c-94be37b60344
名前 / ファイル ライセンス アクション
ChiL_Cryst_ActaF2015.pdf ChiL_Cryst_ActaF2015 (370.8 kB)
ChiL_Cryst_ActaF2015_SupInfo.pdf ChiL_Cryst_ActaF2015_SupInfo (1.8 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2015-12-16
タイトル
言語 en
タイトル Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis
言語
言語 eng
キーワード
主題Scheme Other
主題 chitinase
キーワード
主題Scheme Other
主題 chitinolytic enzyme
キーワード
主題Scheme Other
主題 Chitiniphilus shinanonensis
キーワード
主題Scheme Other
主題 chitin
キーワード
主題Scheme Other
主題 N-acetylglucosamine
キーワード
主題Scheme Other
主題 family 18 glycoside hydrolases
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Ueda, Miruku

× Ueda, Miruku

WEKO 49718

Ueda, Miruku

Search repository
Shimosaka, Makoto

× Shimosaka, Makoto

WEKO 49719

Shimosaka, Makoto

Search repository
Arai, Ryoichi

× Arai, Ryoichi

WEKO 49720

Arai, Ryoichi

Search repository
信州大学研究者総覧へのリンク
氏名 Shimosaka, Makoto
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html
信州大学研究者総覧へのリンク
氏名 Arai, Ryoichi
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.OVkUjFkV.html
出版者
出版者 International Union of Crystallography
引用
内容記述タイプ Other
内容記述 Acta Crystallographica Section F. F7(12):1516-1520 (2015)
書誌情報 Acta Crystallographica Section F

巻 F71, 号 12, p. 1516-1520, 発行日 2015-12
ISSN
収録物識別子タイプ ISSN
収録物識別子 2053-230X
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA12097708
PubMed
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=26625294
関連名称 26625294
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1107/S2053230X15022001
関連名称 10.1107/S2053230X15022001
権利
権利情報 Copyright © International Union of Crystallography
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000369376500011
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