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Rescue of Vitrified-Warmed Bovine Oocytes with Rho-Associated Coiled-Coil Kinase Inhibitor
http://hdl.handle.net/10091/00018690
http://hdl.handle.net/10091/000186905eafedf2-2798-4436-9426-366022efc651
| 名前 / ファイル | ライセンス | アクション |
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Rescue_of_Vitrified-Warmed_Bovine_Oocytes.pdf (489.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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| 公開日 | 2016-03-23 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Rescue of Vitrified-Warmed Bovine Oocytes with Rho-Associated Coiled-Coil Kinase Inhibitor | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| DOI | ||||||||||||||||
| 関連識別子 | https://doi.org/10.1095/biolreprod.113.109769 | |||||||||||||||
| 関連名称 | 10.1095/biolreprod.113.109769 | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題 | apoptosis, bovine oocytes, cryopreservation, Cryotop vitrification, cytoskeleton, microtubule assembly, oocyte, ROCK inhibitor, vitrification | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| タイプ | journal article | |||||||||||||||
| 著者 |
Hwang, In-Sul
× Hwang, In-Sul
× Hara, Hiromasa
× Chung, Hak-Jae
× Hirabayashi, Masumi
× Hochi, Shinichi
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| 信州大学研究者総覧へのリンク | ||||||||||||||||
| 氏名 | Hochi, S | |||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html | |||||||||||||||
| 出版者 | ||||||||||||||||
| 出版者 | SOC STUDY REPRODUCTION | |||||||||||||||
| 引用 | ||||||||||||||||
| 内容記述 | BIOLOGY OF REPRODUCTION. 89(2):26 (2013) | |||||||||||||||
| 書誌情報 |
BIOLOGY OF REPRODUCTION 巻 89, 号 2, p. 26, 発行日 2013-08 |
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| 抄録 | ||||||||||||||||
| 内容記述 | Cryotolerance of matured bovine oocytes is not fully practical even though a promising vitrification procedure with a ultrarapid cooling rate was applied. The present study was conducted to investigate whether recovery culture of vitrified-warmed bovine oocytes with an inhibitor (Y-27632) of Rho-associated coiled-coil kinase (ROCK) can improve the developmental potential after in vitro fertilization (IVF) and in vitro culture. Immediately after warming, almost all oocytes appeared to be morphological normal. Treatment of the postwarming oocytes with 10 μM Y-27632 for 2 h resulted in the significantly higher oocyte survival rate before IVF as well as higher cleavage rate and blastocyst formation rate. Quality analysis of the resultant blastocysts in terms of total cell number and apoptotic cell ratio also showed the positive effect of the Y-27632 treatment. Time-dependent change in mitochondrial activity of the vitrified-warmed oocytes was not influenced by ROCK inhibition during the period of recovery culture. However, the ability of ooplasm to support single-aster formation was improved by the ROCK inhibition. Thus, inhibition of ROCK activity in vitrified-warmed bovine oocytes during a short-term recovery culture can lead to higher developmental competence, probably due to decreased apoptosis and normalized function of the microtubule-organizing center. | |||||||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||||||
| ISSN | ||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||
| 収録物識別子 | 0006-3363 | |||||||||||||||
| 書誌レコードID | ||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||
| 収録物識別子 | AA00565842 | |||||||||||||||
| PubMed | ||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||
| 関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=23782835 | |||||||||||||||
| 関連名称 | 23782835 | |||||||||||||||
| 出版タイプ | ||||||||||||||||
| 出版タイプ | AM | |||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||
| WoS | ||||||||||||||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000323165800004 | |||||||||||||||
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Cite as
Hwang, In-Sul, Hara, Hiromasa, Chung, Hak-Jae, Hirabayashi, Masumi, Hochi, Shinichi, 2013, Rescue of Vitrified-Warmed Bovine Oocytes with Rho-Associated Coiled-Coil Kinase Inhibitor: SOC STUDY REPRODUCTION, 26– p.
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