{"_buckets": {"deposit": "ea7cf1d9-6948-463b-a9f0-b4c38a6b752b"}, "_deposit": {"id": "17924", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "17924"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00017924"}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-11", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "482", "bibliographicPageStart": "476", "bibliographicVolumeNumber": "22", "bibliographic_titles": [{"bibliographic_title": "ZYGOTE"}]}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Although vitrification is a useful technique for preservation of bovine oocytes, the yield of blastocysts derived from the vitrified oocytes is still low. We have recently reported a new type of cryoinjury, multiple aster formation, by which pronuclear migration and development of vitrified\u2013warmed and in vitro-fertilized bovine oocytes are impaired. The aim of the present study was to investigate the effect of glutathione (GSH) content of vitrified bovine oocytes on multiple aster formation and subsequent in vitro development. Treatment of bovine cumulus\u2013oocyte complexes with \u03b2-mercaptoethanol (\u03b2ME) and l-cysteine (Cys) during in vitro maturation resulted in 2.5-fold higher GSH content not only in fresh control but also in vitrified\u2013warmed oocytes. The percentage of normally fertilized zygotes exhibiting sperm aster(s) was \u003e95% in all four groups (with or without \u03b2ME/Cys \u00d7 fresh control or vitrified). The frequency of multiple aster formation in vitrified oocytes (three-fold higher than that in fresh control oocytes) was not affected by the increased level of intracellular GSH with \u03b2ME/Cys. Consequently, the migration and development of pronuclei as well as the yield of blastocysts from vitrified\u2013warmed oocytes (17 versus 41%) were not improved. In addition, there was no effect of increased GSH level on the yield of blastocysts in fresh control groups.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "ZYGOTE. 22(4):476-482 (2014)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Hochi, S", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000344813100007"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "CAMBRIDGE UNIV PRESS"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "23731802"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=23731802", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1017/S0967199413000105"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.1017/S0967199413000105", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0967-1994", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0967-1994", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10958262", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Bovine oocytes@@@Intracellular glutathione@@@beta-Mercaptoethanol@@@Multiple asters@@@Vitrification"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "MATURED PORCINE OOCYTES@@@CYSTEAMINE SUPPLEMENTATION@@@BLASTOCYST DEVELOPMENT@@@BETA-MERCAPTOETHANOL@@@EMBRYO DEVELOPMENT@@@MIXED DISULFIDE@@@THIOL COMPOUNDS@@@MALE PRONUCLEUS@@@MOUSE OOCYTES@@@PIG OOCYTES"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Although vitrification is a useful technique for preservation of bovine oocytes, the yield of blastocysts derived from the vitrified oocytes is still low. We have recently reported a new type of cryoinjury, multiple aster formation, by which pronuclear migration and development of vitrified-warmed and in vitro-fertilized bovine oocytes are impaired. The aim of the present study was to investigate the effect of glutathione (GSH) content of vitrified bovine oocytes on multiple aster formation and subsequent in vitro development. Treatment of bovine cumulus-oocyte complexes with beta-mercaptoethanol (beta ME) and L-cysteine (Cys) during in vitro maturation resulted in 2.5-fold higher GSH content not only in fresh control but also in vitrified-warmed oocytes. The percentage of normally fertilized zygotes exhibiting sperm aster(s) was \u003e95% in all four groups (with or without beta ME/Cys x fresh control or vitrified). The frequency of multiple aster formation in vitrified oocytes (three-fold higher than that in fresh control oocytes) was not affected by the increased level of intracellular GSH with beta ME/Cys. Consequently, the migration and development of pronuclei as well as the yield of blastocysts from vitrified-warmed oocytes (17 versus 41%) were not improved. In addition, there was no effect of increased GSH level on the yield of blastocysts in fresh control groups."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hara,  H"}], "nameIdentifiers": [{"nameIdentifier": "50064", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamane,  I"}], "nameIdentifiers": [{"nameIdentifier": "50065", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Noto,  I"}], "nameIdentifiers": [{"nameIdentifier": "50066", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kagawa,  N"}], "nameIdentifiers": [{"nameIdentifier": "50067", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuwayama,  M"}], "nameIdentifiers": [{"nameIdentifier": "50068", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirabayashi,  M"}], "nameIdentifiers": [{"nameIdentifier": "50069", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hochi,  S"}], "nameIdentifiers": [{"nameIdentifier": "50070", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-03-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Microtubule_assembly_and_in_vitro_development.pdf", "filesize": [{"value": "377.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 377600.0, "url": {"label": "Microtubule_assembly_and_in_vitro_development.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/17924/files/Microtubule_assembly_and_in_vitro_development.pdf"}, "version_id": "947a5391-fa77-49cf-9294-fa1c65682881"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Bovine oocytes", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Intracellular glutathione", "subitem_subject_scheme": "Other"}, {"subitem_subject": "beta-Mercaptoethanol", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Multiple asters", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Vitrification", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Microtubule assembly and in vitro development of bovine oocytes with increased intracellular glutathione level prior to vitrification and in vitro fertilization", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Microtubule assembly and in vitro development of bovine oocytes with increased intracellular glutathione level prior to vitrification and in vitro fertilization"}]}, "item_type_id": "6", "owner": "1", "path": ["1309/1310"], "permalink_uri": "http://hdl.handle.net/10091/00018692", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2016-03-23"}, "publish_date": "2016-03-23", "publish_status": "0", "recid": "17924", "relation": {}, "relation_version_is_last": true, "title": ["Microtubule assembly and in vitro development of bovine oocytes with increased intracellular glutathione level prior to vitrification and in vitro fertilization"], "weko_shared_id": null}