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  1. 080 繊維学部
  2. 0801 学術論文

Microtubule assembly and in vitro development of bovine oocytes with increased intracellular glutathione level prior to vitrification and in vitro fertilization

http://hdl.handle.net/10091/00018692
http://hdl.handle.net/10091/00018692
2b6caad7-7829-4cbc-8d1c-e3ed0f84ab3d
名前 / ファイル ライセンス アクション
Microtubule_assembly_and_in_vitro_development.pdf Microtubule_assembly_and_in_vitro_development.pdf (377.6 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2016-03-23
タイトル
言語 en
タイトル Microtubule assembly and in vitro development of bovine oocytes with increased intracellular glutathione level prior to vitrification and in vitro fertilization
言語
言語 eng
キーワード
主題Scheme Other
主題 Bovine oocytes
キーワード
主題Scheme Other
主題 Intracellular glutathione
キーワード
主題Scheme Other
主題 beta-Mercaptoethanol
キーワード
主題Scheme Other
主題 Multiple asters
キーワード
主題Scheme Other
主題 Vitrification
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Hara, H

× Hara, H

WEKO 50064

Hara, H

Search repository
Yamane, I

× Yamane, I

WEKO 50065

Yamane, I

Search repository
Noto, I

× Noto, I

WEKO 50066

Noto, I

Search repository
Kagawa, N

× Kagawa, N

WEKO 50067

Kagawa, N

Search repository
Kuwayama, M

× Kuwayama, M

WEKO 50068

Kuwayama, M

Search repository
Hirabayashi, M

× Hirabayashi, M

WEKO 50069

Hirabayashi, M

Search repository
Hochi, S

× Hochi, S

WEKO 50070

Hochi, S

Search repository
信州大学研究者総覧へのリンク
氏名 Hochi, S
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html
出版者
出版者 CAMBRIDGE UNIV PRESS
引用
内容記述タイプ Other
内容記述 ZYGOTE. 22(4):476-482 (2014)
書誌情報 ZYGOTE

巻 22, 号 4, p. 476-482, 発行日 2014-11
抄録
内容記述タイプ Abstract
内容記述 Although vitrification is a useful technique for preservation of bovine oocytes, the yield of blastocysts derived from the vitrified oocytes is still low. We have recently reported a new type of cryoinjury, multiple aster formation, by which pronuclear migration and development of vitrified–warmed and in vitro-fertilized bovine oocytes are impaired. The aim of the present study was to investigate the effect of glutathione (GSH) content of vitrified bovine oocytes on multiple aster formation and subsequent in vitro development. Treatment of bovine cumulus–oocyte complexes with β-mercaptoethanol (βME) and l-cysteine (Cys) during in vitro maturation resulted in 2.5-fold higher GSH content not only in fresh control but also in vitrified–warmed oocytes. The percentage of normally fertilized zygotes exhibiting sperm aster(s) was >95% in all four groups (with or without βME/Cys × fresh control or vitrified). The frequency of multiple aster formation in vitrified oocytes (three-fold higher than that in fresh control oocytes) was not affected by the increased level of intracellular GSH with βME/Cys. Consequently, the migration and development of pronuclei as well as the yield of blastocysts from vitrified–warmed oocytes (17 versus 41%) were not improved. In addition, there was no effect of increased GSH level on the yield of blastocysts in fresh control groups.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0967-1994
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10958262
PubMed
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=23731802
関連名称 23731802
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1017/S0967199413000105
関連名称 10.1017/S0967199413000105
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000344813100007
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