{"_buckets": {"deposit": "d1173f10-f97b-4dab-adc4-123f7557bfbd"}, "_deposit": {"id": "18008", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "18008"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00018008", "sets": ["1310"]}, "author_link": ["50341", "50342", "50343", "50344", "50345", "50346", "50347", "50348", "50349"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-08-28", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "819", "bibliographicPageStart": "814", "bibliographicVolumeNumber": "464", "bibliographic_titles": [{"bibliographic_title": "BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Retreat-maker larvae of Stenopsyche marmorata, one of the major caddisfly species in Japan, produce silk threads and adhesives to build food capture nets and protective nests in water. Research on these underwater adhesive silk proteins potentially leads to the development of new functional biofiber materials. Recently, we identified four major S. marmorata silk proteins (Smsps), Smsp-1, Smsp-2, Smsp-3, and Smsp-4 from silk glands of S. marmorata larvae. In this study, we cloned full-length cDNAs of Smsp-2, Smsp-3, and Smsp-4 from the cDNA library of the S. marmorata silk glands to reveal the primary sequences of Smsps. Homology search results of the deduced amino acid sequences indicate that Smsp-2 and Smsp-4 are novel proteins. The Smsp-2 sequence [167 amino acids (aa)] has an array of GYD-rich repeat motifs and two (SX)(4)E motifs. The Smsp-4 sequence (132 aa) contains a number of GW-rich repeat motifs and three (SX)(4)E motifs. The Smsp-3 sequence (248 aa) exhibits high homology with fibroin light chain of other caddisflies. Gene expression analysis of Smsps by real-time PCR suggested that the gene expression of Smsp-1 and Smsp-3 was relatively stable throughout the year, whereas that of Smsp-2 and Smsp-4 varied seasonally. Furthermore, Smsps recombinant protein expression was successfully performed in Escherichia coli. The study provides new molecular insights into caddisfly aquatic silk and its potential for future applications. (C) 2015 Elsevier Inc. All rights reserved.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 464(3):814-819 (2015)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "Tsukada, Masuhiro", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.uhApjFkV.html"}, {"subitem_link_text": "Hirabayashi, Kimio", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.WpTCjekV.html"}, {"subitem_link_text": "Ohkawa, Kousaku", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.gUfejekV.html"}, {"subitem_link_text": "Nomura, Takaomi", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.WhTeONkV.html"}, {"subitem_link_text": "Arai, Ryoichi", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.OVkUjFkV.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000359881900022"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "ACADEMIC PRESS INC ELSEVIER SCIENCE"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "26168724"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=26168724", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/j.bbrc.2015.07.041"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.bbrc.2015.07.041", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Copyright © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0006-291X", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0006-291X", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00564395", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Caddisfly; Trichoptera; Stenopsyche marmorata; Silk protein; Aquatic silk; Smsp"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "ELECTROPHORESIS GELS; UNDERWATER SILK; TRICHOPTERA; FIBROIN; MORI"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Retreat-maker larvae of Stenopsyche marmorata, one of the major caddisfly species in Japan, produce silk threads and adhesives to build food capture nets and protective nests in water. Research on these underwater adhesive silk proteins potentially leads to the development of new functional biofiber materials. Recently, we identified four major S. marmorata silk proteins (Smsps), Smsp-1, Smsp-2, Smsp-3, and Smsp-4 from silk glands of S. marmorata larvae. In this study, we cloned full-length cDNAs of Smsp-2, Smsp-3, and Smsp-4 from the cDNA library of the S. marmorata silk glands to reveal the primary sequences of Smsps. Homology search results of the deduced amino acid sequences indicate that Smsp-2 and Smsp-4 are novel proteins. The Smsp-2 sequence [167 amino acids (aa)] has an array of GYD-rich repeat motifs and two (SX)(4)E motifs. The Smsp-4 sequence (132 aa) contains a number of GW-rich repeat motifs and three (SX)(4)E motifs. The Smsp-3 sequence (248 aa) exhibits high homology with fibroin light chain of other caddisflies. Gene expression analysis of Smsps by real-time PCR suggested that the gene expression of Smsp-1 and Smsp-3 was relatively stable throughout the year, whereas that of Smsp-2 and Smsp-4 varied seasonally. Furthermore, Smsps recombinant protein expression was successfully performed in Escherichia coli. The study provides new molecular insights into caddisfly aquatic silk and its potential for future applications. (C) 2015 Elsevier Inc. All rights reserved."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Bai,  Xue"}], "nameIdentifiers": [{"nameIdentifier": "50341", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakaguchi,  Mayo"}], "nameIdentifiers": [{"nameIdentifier": "50342", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamaguchi,  Yuko"}], "nameIdentifiers": [{"nameIdentifier": "50343", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishihara,  Shiori"}], "nameIdentifiers": [{"nameIdentifier": "50344", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsukada,  Masuhiro"}], "nameIdentifiers": [{"nameIdentifier": "50345", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirabayashi,  Kimio"}], "nameIdentifiers": [{"nameIdentifier": "50346", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohkawa,  Kousaku"}], "nameIdentifiers": [{"nameIdentifier": "50347", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nomura,  Takaomi"}], "nameIdentifiers": [{"nameIdentifier": "50348", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Arai,  Ryoichi"}], "nameIdentifiers": [{"nameIdentifier": "50349", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-04-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Molecular_cloning_gene_expression_analysis_recombinant_protein.pdf", "filesize": [{"value": "1.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 1900000.0, "url": {"label": "Molecular_cloning_gene_expression_analysis_recombinant_protein.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/18008/files/Molecular_cloning_gene_expression_analysis_recombinant_protein.pdf"}, "version_id": "3cecdd85-52e6-43b4-bbd8-9f6250f636e4"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Caddisfly", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Trichoptera", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Stenopsyche marmorata", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Silk protein", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Aquatic silk", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Smsp", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Molecular cloning, gene expression analysis, and recombinant protein expression of novel silk proteins from larvae of a retreat-maker caddisfly, Stenopsyche marmorata", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Molecular cloning, gene expression analysis, and recombinant protein expression of novel silk proteins from larvae of a retreat-maker caddisfly, Stenopsyche marmorata", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1310"], "permalink_uri": "http://hdl.handle.net/10091/00018776", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2016-04-22"}, "publish_date": "2016-04-22", "publish_status": "0", "recid": "18008", "relation": {}, "relation_version_is_last": true, "title": ["Molecular cloning, gene expression analysis, and recombinant protein expression of novel silk proteins from larvae of a retreat-maker caddisfly, Stenopsyche marmorata"], "weko_shared_id": -1}