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  1. 080 繊維学部
  2. 0801 学術論文

Molecular cloning, gene expression analysis, and recombinant protein expression of novel silk proteins from larvae of a retreat-maker caddisfly, Stenopsyche marmorata

http://hdl.handle.net/10091/00018776
http://hdl.handle.net/10091/00018776
26a0a3eb-e815-4865-bd24-283d892a4e5e
名前 / ファイル ライセンス アクション
Molecular_cloning_gene_expression_analysis_recombinant_protein.pdf Molecular_cloning_gene_expression_analysis_recombinant_protein.pdf (1.9 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2016-04-22
タイトル
言語 en
タイトル Molecular cloning, gene expression analysis, and recombinant protein expression of novel silk proteins from larvae of a retreat-maker caddisfly, Stenopsyche marmorata
言語
言語 eng
キーワード
主題Scheme Other
主題 Caddisfly
キーワード
主題Scheme Other
主題 Trichoptera
キーワード
主題Scheme Other
主題 Stenopsyche marmorata
キーワード
主題Scheme Other
主題 Silk protein
キーワード
主題Scheme Other
主題 Aquatic silk
キーワード
主題Scheme Other
主題 Smsp
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Bai, Xue

× Bai, Xue

WEKO 50341

Bai, Xue

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Sakaguchi, Mayo

× Sakaguchi, Mayo

WEKO 50342

Sakaguchi, Mayo

Search repository
Yamaguchi, Yuko

× Yamaguchi, Yuko

WEKO 50343

Yamaguchi, Yuko

Search repository
Ishihara, Shiori

× Ishihara, Shiori

WEKO 50344

Ishihara, Shiori

Search repository
Tsukada, Masuhiro

× Tsukada, Masuhiro

WEKO 50345

Tsukada, Masuhiro

Search repository
Hirabayashi, Kimio

× Hirabayashi, Kimio

WEKO 50346

Hirabayashi, Kimio

Search repository
Ohkawa, Kousaku

× Ohkawa, Kousaku

WEKO 50347

Ohkawa, Kousaku

Search repository
Nomura, Takaomi

× Nomura, Takaomi

WEKO 50348

Nomura, Takaomi

Search repository
Arai, Ryoichi

× Arai, Ryoichi

WEKO 50349

Arai, Ryoichi

Search repository
信州大学研究者総覧へのリンク
氏名 Tsukada, Masuhiro
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.uhApjFkV.html
信州大学研究者総覧へのリンク
氏名 Hirabayashi, Kimio
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.WpTCjekV.html
信州大学研究者総覧へのリンク
氏名 Ohkawa, Kousaku
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.gUfejekV.html
信州大学研究者総覧へのリンク
氏名 Nomura, Takaomi
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.WhTeONkV.html
信州大学研究者総覧へのリンク
氏名 Arai, Ryoichi
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.OVkUjFkV.html
出版者
出版者 ACADEMIC PRESS INC ELSEVIER SCIENCE
引用
内容記述タイプ Other
内容記述 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 464(3):814-819 (2015)
書誌情報 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

巻 464, 号 3, p. 814-819, 発行日 2015-08-28
抄録
内容記述タイプ Abstract
内容記述 Retreat-maker larvae of Stenopsyche marmorata, one of the major caddisfly species in Japan, produce silk threads and adhesives to build food capture nets and protective nests in water. Research on these underwater adhesive silk proteins potentially leads to the development of new functional biofiber materials. Recently, we identified four major S. marmorata silk proteins (Smsps), Smsp-1, Smsp-2, Smsp-3, and Smsp-4 from silk glands of S. marmorata larvae. In this study, we cloned full-length cDNAs of Smsp-2, Smsp-3, and Smsp-4 from the cDNA library of the S. marmorata silk glands to reveal the primary sequences of Smsps. Homology search results of the deduced amino acid sequences indicate that Smsp-2 and Smsp-4 are novel proteins. The Smsp-2 sequence [167 amino acids (aa)] has an array of GYD-rich repeat motifs and two (SX)(4)E motifs. The Smsp-4 sequence (132 aa) contains a number of GW-rich repeat motifs and three (SX)(4)E motifs. The Smsp-3 sequence (248 aa) exhibits high homology with fibroin light chain of other caddisflies. Gene expression analysis of Smsps by real-time PCR suggested that the gene expression of Smsp-1 and Smsp-3 was relatively stable throughout the year, whereas that of Smsp-2 and Smsp-4 varied seasonally. Furthermore, Smsps recombinant protein expression was successfully performed in Escherichia coli. The study provides new molecular insights into caddisfly aquatic silk and its potential for future applications. (C) 2015 Elsevier Inc. All rights reserved.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0006-291X
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00564395
PubMed
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=26168724
関連名称 26168724
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.bbrc.2015.07.041
関連名称 10.1016/j.bbrc.2015.07.041
権利
権利情報 Copyright © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000359881900022
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