| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2016-04-22 |
| タイトル |
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タイトル |
Molecular cloning, gene expression analysis, and recombinant protein expression of novel silk proteins from larvae of a retreat-maker caddisfly, Stenopsyche marmorata |
| 言語 |
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言語 |
eng |
| DOI |
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関連識別子 |
https://doi.org/10.1016/j.bbrc.2015.07.041 |
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関連名称 |
10.1016/j.bbrc.2015.07.041 |
| キーワード |
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主題 |
Caddisfly, Trichoptera, Stenopsyche marmorata, Silk protein, Aquatic silk, Smsp |
| 資源タイプ |
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|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Bai, Xue
Sakaguchi, Mayo
Yamaguchi, Yuko
Ishihara, Shiori
Tsukada, Masuhiro
Hirabayashi, Kimio
Ohkawa, Kousaku
Nomura, Takaomi
Arai, Ryoichi
|
| 信州大学研究者総覧へのリンク |
|
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氏名 |
Tsukada, Masuhiro |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.uhApjFkV.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Hirabayashi, Kimio |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.WpTCjekV.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Ohkawa, Kousaku |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.gUfejekV.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Nomura, Takaomi |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.WhTeONkV.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Arai, Ryoichi |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.OVkUjFkV.html |
| 出版者 |
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出版者 |
ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 引用 |
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内容記述 |
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 464(3):814-819 (2015) |
| 書誌情報 |
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
巻 464,
号 3,
p. 814-819,
発行日 2015-08-28
|
| 抄録 |
|
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内容記述 |
Retreat-maker larvae of Stenopsyche marmorata, one of the major caddisfly species in Japan, produce silk threads and adhesives to build food capture nets and protective nests in water. Research on these underwater adhesive silk proteins potentially leads to the development of new functional biofiber materials. Recently, we identified four major S. marmorata silk proteins (Smsps), Smsp-1, Smsp-2, Smsp-3, and Smsp-4 from silk glands of S. marmorata larvae. In this study, we cloned full-length cDNAs of Smsp-2, Smsp-3, and Smsp-4 from the cDNA library of the S. marmorata silk glands to reveal the primary sequences of Smsps. Homology search results of the deduced amino acid sequences indicate that Smsp-2 and Smsp-4 are novel proteins. The Smsp-2 sequence [167 amino acids (aa)] has an array of GYD-rich repeat motifs and two (SX)(4)E motifs. The Smsp-4 sequence (132 aa) contains a number of GW-rich repeat motifs and three (SX)(4)E motifs. The Smsp-3 sequence (248 aa) exhibits high homology with fibroin light chain of other caddisflies. Gene expression analysis of Smsps by real-time PCR suggested that the gene expression of Smsp-1 and Smsp-3 was relatively stable throughout the year, whereas that of Smsp-2 and Smsp-4 varied seasonally. Furthermore, Smsps recombinant protein expression was successfully performed in Escherichia coli. The study provides new molecular insights into caddisfly aquatic silk and its potential for future applications. (C) 2015 Elsevier Inc. All rights reserved. |
| 資源タイプ（コンテンツの種類） |
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0006-291X |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00564395 |
| PubMed |
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識別子タイプ |
PMID |
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関連識別子 |
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=26168724 |
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関連名称 |
26168724 |
| 権利 |
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|
権利情報 |
Copyright © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 出版タイプ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| WoS |
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|
URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000359881900022 |
Molecular_cloning_gene_expression_analysis_recombinant_protein.pdf (1.9 MB)
