{"_buckets": {"deposit": "44c3b824-0521-42eb-b7f3-5cba8e7f28af"}, "_deposit": {"id": "18015", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "18015"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00018015"}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "8", "bibliographicPageEnd": "1511", "bibliographicPageStart": "1490", "bibliographicVolumeNumber": "56", "bibliographic_titles": [{"bibliographic_title": "PLANT AND CELL PHYSIOLOGY"}]}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Gene expression during arbuscular mycorrhizal development is highly orchestrated in both plants and arbuscular mycorrhizal fungi. To elucidate the gene expression profiles of the symbiotic association, we performed a digital gene expression analysis of Lotus japonicus and Rhizophagus irregularis using a HiSeq 2000 next-generation sequencer with a Cufflinks assembly and de novo transcriptome assembly. There were 3,641 genes differentially expressed during arbuscular mycorrhizal development in L. japonicus, approximately 80% of which were up-regulated. The up-regulated genes included secreted proteins, transporters, proteins involved in lipid and amino acid metabolism, ribosomes and histones. We also detected many genes that were differentially expressed in small-secreted peptides and transcription factors, which may be involved in signal transduction or transcription regulation during symbiosis. Coregulated genes between arbuscular mycorrhizal and root nodule symbiosis were not particularly abundant, but transcripts encoding for membrane traffic-related proteins, transporters and iron transport-related proteins were found to be highly co-up-regulated. In transcripts of arbuscular mycorrhizal fungi, expansion of cytochrome P450 was observed, which may contribute to various metabolic pathways required to accommodate roots and soil. 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To elucidate the gene expression profiles of the symbiotic association, we performed a digital gene expression analysis of Lotus japonicus and Rhizophagus irregularis using a HiSeq 2000 next-generation sequencer with a Cufflinks assembly and de novo transcriptome assembly. There were 3,641 genes differentially expressed during arbuscular mycorrhizal development in L. japonicus, approximately 80% of which were up-regulated. The up-regulated genes included secreted proteins, transporters, proteins involved in lipid and amino acid metabolism, ribosomes and histones. We also detected many genes that were differentially expressed in small-secreted peptides and transcription factors, which may be involved in signal transduction or transcription regulation during symbiosis. Coregulated genes between arbuscular mycorrhizal and root nodule symbiosis were not particularly abundant, but transcripts encoding for membrane traffic-related proteins, transporters and iron transport-related proteins were found to be highly co-up-regulated. In transcripts of arbuscular mycorrhizal fungi, expansion of cytochrome P450 was observed, which may contribute to various metabolic pathways required to accommodate roots and soil. The comprehensive gene expression data of both plants and arbuscular mycorrhizal fungi provide a powerful platform for investigating the functional and molecular mechanisms underlying arbuscular mycorrhizal symbiosis."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Handa,  Yoshihiro"}], "nameIdentifiers": [{"nameIdentifier": "50367", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishide,  Hiroyo"}], "nameIdentifiers": [{"nameIdentifier": "50368", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takeda,  Naoya"}], "nameIdentifiers": [{"nameIdentifier": "50369", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki,  Yutaka"}], "nameIdentifiers": [{"nameIdentifier": "50370", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawaguchi,  Masayoshi"}], "nameIdentifiers": [{"nameIdentifier": "50371", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saito,  Katsuharu"}], "nameIdentifiers": [{"nameIdentifier": "50372", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-05-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "RNA-seq_Transcriptional_Profiling_Arbuscular_Mycorrhiza.pdf", "filesize": [{"value": "1.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1600000.0, "url": {"label": "RNA-seq_Transcriptional_Profiling_Arbuscular_Mycorrhiza.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/18015/files/RNA-seq_Transcriptional_Profiling_Arbuscular_Mycorrhiza.pdf"}, "version_id": "6cc7e5da-ce91-4dde-b071-768ce4747c8e"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Arbuscular mycorrhiza", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Lotus japonicus", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Rhizophagus irregularis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Root nodule", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Symbiosis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Transcriptome", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "RNA-seq Transcriptional Profiling of an Arbuscular Mycorrhiza Provides Insights into Regulated and Coordinated Gene Expression in Lotus japonicus and Rhizophagus irregularis", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "RNA-seq Transcriptional Profiling of an Arbuscular Mycorrhiza Provides Insights into Regulated and Coordinated Gene Expression in Lotus japonicus and Rhizophagus irregularis"}]}, "item_type_id": "6", "owner": "1", "path": ["1016/1017"], "permalink_uri": "http://hdl.handle.net/10091/00018783", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2016-04-22"}, "publish_date": "2016-04-22", "publish_status": "0", "recid": "18015", "relation": {}, "relation_version_is_last": true, "title": ["RNA-seq Transcriptional Profiling of an Arbuscular Mycorrhiza Provides Insights into Regulated and Coordinated Gene Expression in Lotus japonicus and Rhizophagus irregularis"], "weko_shared_id": null}