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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

A third glucose uptake bypass in Corynebacterium glutamicum ATCC 31833

http://hdl.handle.net/10091/00018787
http://hdl.handle.net/10091/00018787
333c42b5-cc04-494e-a8fa-02d218c3ce7b
名前 / ファイル ライセンス アクション
A_third_glucose_uptake_bypass.pdf A_third_glucose_uptake_bypass.pdf (868.4 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2016-04-22
タイトル
言語 en
タイトル A third glucose uptake bypass in Corynebacterium glutamicum ATCC 31833
言語
言語 eng
キーワード
主題Scheme Other
主題 Corynebacterium glutamicum
キーワード
主題Scheme Other
主題 Phosphotransferase system
キーワード
主題Scheme Other
主題 Glucose uptake bypass
キーワード
主題Scheme Other
主題 beta-Glucoside-PTS
キーワード
主題Scheme Other
主題 bglF
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Ikeda, Masato

× Ikeda, Masato

WEKO 50396

Ikeda, Masato

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Noguchi, Norio

× Noguchi, Norio

WEKO 50397

Noguchi, Norio

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Ohshita, Masakazu

× Ohshita, Masakazu

WEKO 50398

Ohshita, Masakazu

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Senoo, Akihiro

× Senoo, Akihiro

WEKO 50399

Senoo, Akihiro

Search repository
Mitsuhashi, Satoshi

× Mitsuhashi, Satoshi

WEKO 50400

Mitsuhashi, Satoshi

Search repository
Takeno, Seiki

× Takeno, Seiki

WEKO 50401

Takeno, Seiki

Search repository
信州大学研究者総覧へのリンク
氏名 Ikeda, Masato
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html
信州大学研究者総覧へのリンク
氏名 Takeno, Seiki
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html
出版者
出版者 SPRINGER
引用
内容記述タイプ Other
内容記述 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 99(6):2741-2750 (2015)
書誌情報 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

巻 99, 号 6, p. 2741-2750, 発行日 2015-03
抄録
内容記述タイプ Abstract
内容記述 In Corynebacterium glutamicum, the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) has long been the only known glucose uptake system, but we recently found suppressor mutants emerging from a PTS-negative strain of C. glutamicum ATCC 31833 on glucose agar plates, and identified two alternative potential glucose uptake systems, the myo-inositol transporters encoded by iolT1 and iolT2. The expression of either gene renders the PTS-negative strain WT Delta ptsH capable of growing on glucose. In the present study, we found a suppressor strain that still grew on glucose even after the iolT1 and iolT2 genes were both disrupted under the PTS-negative background. Whole-genome sequencing of the suppressor strain SPH1 identified a G-to-T exchange at 134 bp upstream of the bglF gene encoding an EII component of the beta-glucoside-PTS, which is found in limited wild-type strains of C. glutamicum. Introduction of the mutation into strain WT Delta ptsH allowed the PTS-negative strain to grow on glucose. Reverse transcription-quantitative PCR analysis revealed that the mutation upregulates the bglF gene by approximately 11-fold. Overexpression of bglF under the gapA promoter in strain WT Delta ptsH rendered the strain capable of growing on glucose, and deletion of bglF in strain SPH1 abolished the growth again, proving that bglF is responsible for glucose uptake in the suppressor strain. Simultaneous disruption of three glucokinase genes, glk (Cgl2185, NCgl2105), ppgK (Cgl1910, NCgl1835), and Cgl2647 (NCgl2558), in strain SPH1 resulted in no growth on glucose. Plasmid-mediated expression of any of the three genes in the triple-knockout mutant restored the growth on glucose. These results indicate that C. glutamicum ATCC 31833 has an additional non-PTS glucose uptake route consisting of the bglF-specified EII permease and native glucokinases.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0175-7598
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10503386
PubMed
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/25549619/
関連名称 25549619
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1007/s00253-014-6323-1
関連名称 10.1007/s00253-014-6323-1
権利
権利情報 The original publication is available at www.springerlink.com
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000350362500024
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