WEKO3
アイテム
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The expression of either gene renders the PTS-negative strain WT Delta ptsH capable of growing on glucose. In the present study, we found a suppressor strain that still grew on glucose even after the iolT1 and iolT2 genes were both disrupted under the PTS-negative background. Whole-genome sequencing of the suppressor strain SPH1 identified a G-to-T exchange at 134 bp upstream of the bglF gene encoding an EII component of the beta-glucoside-PTS, which is found in limited wild-type strains of C. glutamicum. Introduction of the mutation into strain WT Delta ptsH allowed the PTS-negative strain to grow on glucose. Reverse transcription-quantitative PCR analysis revealed that the mutation upregulates the bglF gene by approximately 11-fold. Overexpression of bglF under the gapA promoter in strain WT Delta ptsH rendered the strain capable of growing on glucose, and deletion of bglF in strain SPH1 abolished the growth again, proving that bglF is responsible for glucose uptake in the suppressor strain. Simultaneous disruption of three glucokinase genes, glk (Cgl2185, NCgl2105), ppgK (Cgl1910, NCgl1835), and Cgl2647 (NCgl2558), in strain SPH1 resulted in no growth on glucose. Plasmid-mediated expression of any of the three genes in the triple-knockout mutant restored the growth on glucose. 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A third glucose uptake bypass in Corynebacterium glutamicum ATCC 31833
http://hdl.handle.net/10091/00018787
http://hdl.handle.net/10091/00018787333c42b5-cc04-494e-a8fa-02d218c3ce7b
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-04-22 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | A third glucose uptake bypass in Corynebacterium glutamicum ATCC 31833 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Corynebacterium glutamicum | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Phosphotransferase system | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Glucose uptake bypass | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | beta-Glucoside-PTS | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | bglF | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Ikeda, Masato
× Ikeda, Masato× Noguchi, Norio× Ohshita, Masakazu× Senoo, Akihiro× Mitsuhashi, Satoshi× Takeno, Seiki |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Ikeda, Masato | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Takeno, Seiki | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html | |||||
出版者 | ||||||
出版者 | SPRINGER | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 99(6):2741-2750 (2015) | |||||
書誌情報 |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 巻 99, 号 6, p. 2741-2750, 発行日 2015-03 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In Corynebacterium glutamicum, the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) has long been the only known glucose uptake system, but we recently found suppressor mutants emerging from a PTS-negative strain of C. glutamicum ATCC 31833 on glucose agar plates, and identified two alternative potential glucose uptake systems, the myo-inositol transporters encoded by iolT1 and iolT2. The expression of either gene renders the PTS-negative strain WT Delta ptsH capable of growing on glucose. In the present study, we found a suppressor strain that still grew on glucose even after the iolT1 and iolT2 genes were both disrupted under the PTS-negative background. Whole-genome sequencing of the suppressor strain SPH1 identified a G-to-T exchange at 134 bp upstream of the bglF gene encoding an EII component of the beta-glucoside-PTS, which is found in limited wild-type strains of C. glutamicum. Introduction of the mutation into strain WT Delta ptsH allowed the PTS-negative strain to grow on glucose. Reverse transcription-quantitative PCR analysis revealed that the mutation upregulates the bglF gene by approximately 11-fold. Overexpression of bglF under the gapA promoter in strain WT Delta ptsH rendered the strain capable of growing on glucose, and deletion of bglF in strain SPH1 abolished the growth again, proving that bglF is responsible for glucose uptake in the suppressor strain. Simultaneous disruption of three glucokinase genes, glk (Cgl2185, NCgl2105), ppgK (Cgl1910, NCgl1835), and Cgl2647 (NCgl2558), in strain SPH1 resulted in no growth on glucose. Plasmid-mediated expression of any of the three genes in the triple-knockout mutant restored the growth on glucose. These results indicate that C. glutamicum ATCC 31833 has an additional non-PTS glucose uptake route consisting of the bglF-specified EII permease and native glucokinases. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0175-7598 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10503386 | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | https://pubmed.ncbi.nlm.nih.gov/25549619/ | |||||
関連名称 | 25549619 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1007/s00253-014-6323-1 | |||||
関連名称 | 10.1007/s00253-014-6323-1 | |||||
権利 | ||||||
権利情報 | The original publication is available at www.springerlink.com | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000350362500024 |