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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

L-Lysine production independent of the oxidative pentose phosphate pathway by Corynebacterium glutamicum with the Streptococcus mutans gapN gene

http://hdl.handle.net/10091/00018968
8c399af5-68a9-408b-a7f7-7765b96c2998
名前 / ファイル ライセンス アクション
L-lysine_production_independent_oxidative.pdf L-lysine_production_independent_oxidative.pdf (1.4 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2016-07-07
タイトル
言語 en
タイトル L-Lysine production independent of the oxidative pentose phosphate pathway by Corynebacterium glutamicum with the Streptococcus mutans gapN gene
言語
言語 eng
キーワード
主題Scheme Other
主題 Corynebacterium glutamicum
キーワード
主題Scheme Other
主題 Glyceraldehyde 3-phosphate dehydrogenases
キーワード
主題Scheme Other
主題 NADPH
キーワード
主題Scheme Other
主題 Glycolytic pathway
キーワード
主題Scheme Other
主題 Pentose phosphate pathway
キーワード
主題Scheme Other
主題 L-Lysine production
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Takeno, Seiki

× Takeno, Seiki

WEKO 50833

Takeno, Seiki

Search repository
Hori, Kazumasa

× Hori, Kazumasa

WEKO 50834

Hori, Kazumasa

Search repository
Ohtani, Sachiko

× Ohtani, Sachiko

WEKO 50835

Ohtani, Sachiko

Search repository
Mimura, Akinori

× Mimura, Akinori

WEKO 50836

Mimura, Akinori

Search repository
Mitsuhashi, Satoshi

× Mitsuhashi, Satoshi

WEKO 50837

Mitsuhashi, Satoshi

Search repository
Ikeda, Masato

× Ikeda, Masato

WEKO 50838

Ikeda, Masato

Search repository
信州大学研究者総覧へのリンク
氏名 Takeno, Seiki
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html
信州大学研究者総覧へのリンク
氏名 Ikeda, Masato
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html
出版者
出版者 ELSEVIER
引用
内容記述タイプ Other
内容記述 METABOLIC ENGINEERING. 37:1-10 (2016)
書誌情報 METABOLIC ENGINEERING

巻 37, p. 1-10, 発行日 2016-09
内容記述
内容記述タイプ Other
内容記述 Published online 29 March 2016
抄録
内容記述タイプ Abstract
内容記述 We have recently developed a Corynebacterium glutamicum strain that generates NADPH via the glycolytic pathway by replacing endogenous NAD-dependent glyceraldehyde 3-phosphate dehydrogenase (GapA) with a nonphosphorylating NADP-dependent glyceraldehyde 3-phosphate dehydrogenase (GapN) from Streptococcus mutans. Strain RE2, a suppressor mutant spontaneously isolated for its improved growth on glucose from the engineered strain, was proven to be a high-potential host for l-lysine production (Takeno et al., 2010). In this study, the suppressor mutation was identified to be a point mutation in rho encoding the transcription termination factor Rho. Strain RE2 still showed retarded growth despite the mutation rho696. Our strategy for reconciling improved growth with a high level of l-lysine production was to use GapA together with GapN only in the early growth phase, and subsequently shift this combination-type glycolysis to one that depends only on GapN in the rest of the growth phase. To achieve this, we expressed gapA under the myo-inositol-inducible promoter of iolT1 encoding a myo-inositol transporter in strain RE2. The resulting strain RE2Aiol was engineered into an l-lysine producer by introduction of a plasmid carrying the desensitized lysC, followed by examination for culture conditions with myo-inositol supplementation. We found that as a higher concentration of myo-inositol was added to the seed culture, the following fermentation period became shorter while maintaining a high level of l-lysine production. This finally reached a fermentation period comparable to that of the control GapA strain, and yielded a 1.5-fold higher production rate compared with strain RE2. The transcript level of gapA, as well as the GapA activity, in the early growth phase increased in proportion to the myo-inositol concentration and then fell to low levels in the subsequent growth phase, indicating that improved growth was a result of increased GapA activity, especially in the early growth phase. Moreover, blockade of the pentose phosphate pathway through a defect in glucose 6-phosphate dehydrogenase did not significantly affect l-lysine production in the engineered GapN strains, while a drastic decrease in l-lysine production was observed for the control GapA strain. Determination of the intracellular NADPH/NADP+ ratios revealed that the ratios in the engineered strains were significantly higher than the ratio of the control GapA strain irrespective of the pentose phosphate pathway. These results demonstrate that our strain engineering strategy allows efficient l-lysine production independent of the oxidative pentose phosphate pathway.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 1096-7176
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11589605
PubMed
関連識別子
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=27044449
関連名称
関連名称 27044449
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.ymben.2016.03.007
関連名称
関連名称 10.1016/j.ymben.2016.03.007
権利
権利情報 © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000377983400001
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