{"_buckets": {"deposit": "dcb8a929-cd43-4977-ad8f-5cc48196d3ff"}, "_deposit": {"id": "18980", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "18980"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00018980"}, "item_14_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-03-31", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_14_date_granted_8": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5", "attribute_value_mlt": [{"subitem_dategranted": "2017-03-31"}]}, "item_14_degree_grantor_10": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u6a5f\u95a2", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "\u4fe1\u5dde\u5927\u5b66(Shinshu university)"}]}]}, "item_14_degree_name_9": {"attribute_name": "\u5b66\u4f4d\u540d", "attribute_value_mlt": [{"subitem_degreename": "\u535a\u58eb\uff08\u533b\u5b66\uff09"}]}, "item_14_description_26": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u96d1\u8a8c\u306b\u767a\u8868\u3002ONCOLOGY REPORTS. 37(2):1020-1026 (2017); doi:10.3892/or.2016.5287.", "subitem_description_type": "Other"}]}, "item_14_description_37": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Thesis", "subitem_description_type": "Other"}]}, "item_14_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "\u6d45\u9999\u3000\u5fd7\u7a42. A novel, rapid point-of-care test for lung cancer patients to detect epidermal growth factor receptor gene mutations by using real-time droplet-PCR and fresh liquid cytology specimens\uff08\u80ba\u764c\u60a3\u8005\u306b\u304a\u3051\u308b\u4e0a\u76ae\u6210\u9577\u56e0\u5b50\u53d7\u5bb9\u4f53\u907a\u4f1d\u5b50\u5909\u7570\u691c\u51fa\u306e\u305f\u3081\u306e\u6db2\u6ef4\u578b\u9ad8\u901fPCR\u88c5\u7f6e\u3068\u65b0\u9bae\u6db2\u72b6\u7d30\u80de\u8a3a\u691c\u4f53\u3092\u7528\u3044\u305f\u65b0\u898f\u8fc5\u901f\u30dd\u30a4\u30f3\u30c8\u30fb\u30aa\u30d6\u30fb\u30b1\u30a2\u30fb\u30c6\u30b9\u30c8\uff09. \u4fe1\u5dde\u5927\u5b66, 2017, \u535a\u58eb\u8ad6\u6587.", "subitem_description_type": "Other"}]}, "item_14_dissertation_number_7": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u756a\u53f7", "attribute_value_mlt": [{"subitem_dissertationnumber": "13601\u7532\u7b2c1080\u53f7"}]}, "item_14_link_74": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000393086900044"}]}, "item_14_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u4fe1\u5dde\u5927\u5b66"}]}, "item_14_relation_54": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "27922678"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=27922678", "subitem_relation_type_select": "PMID"}}]}, "item_14_relation_55": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.3892/or.2016.5287"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.3892/or.2016.5287", "subitem_relation_type_select": "DOI"}}]}, "item_14_select_71": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_14_text_11": {"attribute_name": "\u5b66\u4f4d\u306e\u533a\u5206", "attribute_value_mlt": [{"subitem_text_value": "Doctoral"}]}, "item_14_text_12": {"attribute_name": "\u5b66\u4f4d\u306e\u5206\u91ce", "attribute_value_mlt": [{"subitem_text_value": "\u533b\u5b66"}]}, "item_14_text_13": {"attribute_name": "\u5b66\u4f4d\u306e\u5831\u544a\u756a\u53f7", "attribute_value_mlt": [{"subitem_text_value": "\u7532\u7b2c1080\u53f7"}]}, "item_14_text_76": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "real-time droplet-PCR; point-of-care testing; lung cancer; EGFR; rapid assay; fresh liquid cytology specimen; bronchial lavage fluid; pleural effusion; cardiac effusion"}]}, "item_14_text_77": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "POLYMERASE-CHAIN-REACTION; TYROSINE KINASE INHIBITORS; RARA FUSION GENE; EGFR MUTATIONS; SENSITIVE DETECTION; INFLUENZA-VIRUS; GEFITINIB; POLYMORPHISMS; CHEMOTHERAPY; ASSOCIATION"}]}, "item_14_textarea_75": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Epidermal growth factor receptor gene (EGFR) mutations are associated with response to tyrosine kinase inhibitors (TKIs) in patients with non-small cell lung cancer (NSCLC). We developed a novel, rapid EGFR mutation assay using a real-time droplet-polymerase chain reaction machine (EGFR d-PCR assay). The purpose of this study was to validate the performance of the EGFR d-PCR assay using fresh liquid cytology specimens. We analyzed three major EGFR mutations (L858R in exon 21, E746_A750del in exon 19 and T790M in exon 20) in 80 fresh liquid cytology specimens of adenocarcinoma (ADC) or NSCLC-not otherwise specified (NOS) via the EGFR d-PCR assay and conventional real-time PCR assay using the therascreen (R) EGFR RGQ PCR kit (Therascreen assay). In addition, we performed sensitivity assays using cell lines with EGFR mutations. The EGFR d-PCR assay detected 16 L858Rs, 8 E746_A750dels and 1 T790M mutation and the Therascreen assay detected 16 L858Rs, 11 deletions in exon 19 and 1 T790M mutation. The results were concordant between the two assays. The reaction time of the EGFR d-PCR assay was 8 min and 10 sec, but that of the Therascreen assay was 1 h and 45 min. Sensitivity, as assessed by the detection limit of the EGFR d-PCR assay was 0.5, 0.05 and 0.5% for L858R, E746_A750del and T790M, respectively. The EGFR d-PCR assay markedly reduced the detection time of major EGFR mutations with high sensitivity compared with the conventional Therascreen assay and is expected to expedite EGFR-TKI therapy for lung cancer patients, especially those in advanced stages."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u6d45\u9999, \u5fd7\u7a42"}], "nameIdentifiers": [{"nameIdentifier": "103714", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-06-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "13MH001A_shinsa.pdf", "filesize": [{"value": "103.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 103500.0, "url": {"label": "\u5185\u5bb9\u306e\u8981\u65e8", "url": "https://soar-ir.repo.nii.ac.jp/record/18980/files/13MH001A_shinsa.pdf"}, "version_id": "2c26aa7a-24ff-4fab-8610-9739c1c0ae14"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-06-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "13MH001A_yoshi.pdf", "filesize": [{"value": "141.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 141600.0, "url": {"label": "\u5be9\u67fb\u7d50\u679c\u306e\u8981\u65e8", "url": "https://soar-ir.repo.nii.ac.jp/record/18980/files/13MH001A_yoshi.pdf"}, "version_id": "15ce7b40-765d-47b8-8f71-fbc2b672a26e"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-09-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "13MH001A_ronbun.pdf", "filesize": [{"value": "401.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 401400.0, "url": {"label": "\u535a\u58eb\u8ad6\u6587\u306e\u5168\u6587", "url": "https://soar-ir.repo.nii.ac.jp/record/18980/files/13MH001A_ronbun.pdf"}, "version_id": "6151c5a2-468a-4c03-a0c2-cbb4631be3e8"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "A novel, rapid point-of-care test for lung cancer patients to detect epidermal growth factor receptor gene mutations by using real-time droplet-PCR and fresh liquid cytology specimens\uff08\u80ba\u764c\u60a3\u8005\u306b\u304a\u3051\u308b\u4e0a\u76ae\u6210\u9577\u56e0\u5b50\u53d7\u5bb9\u4f53\u907a\u4f1d\u5b50\u5909\u7570\u691c\u51fa\u306e\u305f\u3081\u306e\u6db2\u6ef4\u578b\u9ad8\u901fPCR\u88c5\u7f6e\u3068\u65b0\u9bae\u6db2\u72b6\u7d30\u80de\u8a3a\u691c\u4f53\u3092\u7528\u3044\u305f\u65b0\u898f\u8fc5\u901f\u30dd\u30a4\u30f3\u30c8\u30fb\u30aa\u30d6\u30fb\u30b1\u30a2\u30fb\u30c6\u30b9\u30c8\uff09", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "A novel, rapid point-of-care test for lung cancer patients to detect epidermal growth factor receptor gene mutations by using real-time droplet-PCR and fresh liquid cytology specimens\uff08\u80ba\u764c\u60a3\u8005\u306b\u304a\u3051\u308b\u4e0a\u76ae\u6210\u9577\u56e0\u5b50\u53d7\u5bb9\u4f53\u907a\u4f1d\u5b50\u5909\u7570\u691c\u51fa\u306e\u305f\u3081\u306e\u6db2\u6ef4\u578b\u9ad8\u901fPCR\u88c5\u7f6e\u3068\u65b0\u9bae\u6db2\u72b6\u7d30\u80de\u8a3a\u691c\u4f53\u3092\u7528\u3044\u305f\u65b0\u898f\u8fc5\u901f\u30dd\u30a4\u30f3\u30c8\u30fb\u30aa\u30d6\u30fb\u30b1\u30a2\u30fb\u30c6\u30b9\u30c8\uff09"}]}, "item_type_id": "14", "owner": "1", "path": ["461/848/849"], "permalink_uri": "http://hdl.handle.net/10091/00019742", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2017-06-06"}, "publish_date": "2017-06-06", "publish_status": "0", "recid": "18980", "relation": {}, "relation_version_is_last": true, "title": ["A novel, rapid point-of-care test for lung cancer patients to detect epidermal growth factor receptor gene mutations by using real-time droplet-PCR and fresh liquid cytology specimens\uff08\u80ba\u764c\u60a3\u8005\u306b\u304a\u3051\u308b\u4e0a\u76ae\u6210\u9577\u56e0\u5b50\u53d7\u5bb9\u4f53\u907a\u4f1d\u5b50\u5909\u7570\u691c\u51fa\u306e\u305f\u3081\u306e\u6db2\u6ef4\u578b\u9ad8\u901fPCR\u88c5\u7f6e\u3068\u65b0\u9bae\u6db2\u72b6\u7d30\u80de\u8a3a\u691c\u4f53\u3092\u7528\u3044\u305f\u65b0\u898f\u8fc5\u901f\u30dd\u30a4\u30f3\u30c8\u30fb\u30aa\u30d6\u30fb\u30b1\u30a2\u30fb\u30c6\u30b9\u30c8\uff09"], "weko_shared_id": 3}