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Thymidine-dependent SCVs (TD-SCVs) are unable to synthesize thymidine; hence, these variants fail to grow in a medium without thymidine. In this study, we used 10 TD-SCVs of Staphylococcus aureus, of which four strains possessed mecA. We compared the efficacy of a newly modified medium containing thymidine for the detection of TD-SCVs of methicillin-resistant S. aureus (MRSA) to the efficacy of routinely used laboratory media. We observed that none of the 10 TD-SCVs of S. aureus grew in Mueller-Hinton agar, and four TD-SCVs of MRSA failed to grow on all MRSA screening media, except for the ChromID (TM) MRSA medium. Laboratory tests conducted using medium with thymidine incorporated showed that thymidine did not affect the minimum inhibitory concentrations of oxacillin and cefoxitin for clinical isolates of S. aureus, and was able to detect MRSA, including TD-SCVs. 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  1. 050 医学部, 大学院医学系研究科
  2. 0507 学位論文
  3. 博士(医学)

Addition of thymidine to culture media for accurate examination of thymidine-dependent small-colony variants of methicillin-resistant Staphylococcus aureus: A pilot study(メチシリン耐性 Staphylococcus aureus チミジン依存性変異株の正確な検査を行うために培地へチミジンを添加する試験的研究)

http://hdl.handle.net/10091/00019745
http://hdl.handle.net/10091/00019745
464c48e1-e57c-4351-b02c-9034016ee861
名前 / ファイル ライセンス アクション
13MH038K_shinsa.pdf 内容の要旨 (85.3 kB)
13MH038K_yoshi.pdf 審査結果の要旨 (171.9 kB)
13MH038K_ronbun.pdf 博士論文の全文 (488.6 kB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2017-06-06
タイトル
言語 en
タイトル Addition of thymidine to culture media for accurate examination of thymidine-dependent small-colony variants of methicillin-resistant Staphylococcus aureus: A pilot study(メチシリン耐性 Staphylococcus aureus チミジン依存性変異株の正確な検査を行うために培地へチミジンを添加する試験的研究)
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_db06
タイプ doctoral thesis
著者 堀内, 一樹

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堀内, 一樹

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出版者
出版者 信州大学
引用
内容記述タイプ Other
内容記述 堀内 一樹. Addition of thymidine to culture media for accurate examination of thymidine-dependent small-colony variants of methicillin-resistant Staphylococcus aureus: A pilot study(メチシリン耐性 Staphylococcus aureus チミジン依存性変異株の正確な検査を行うために培地へチミジンを添加する試験的研究). 信州大学, 2017, 博士論文.
書誌情報
発行日 2017-03-31
学位授与番号
学位授与番号 13601甲第1084号
学位授与年月日
学位授与年月日 2017-03-31
学位名
学位名 博士(医学)
学位授与機関
学位授与機関名 信州大学(Shinshu university)
学位の区分
Doctoral
学位の分野
医学
学位の報告番号
甲第1084号
内容記述
内容記述タイプ Other
内容記述 雑誌に発表。JOURNAL OF MICROBIOLOGICAL METHODS. 110:40-44 (2015); doi:10.1016/j.mimet.2015.01.007.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Thesis
PubMed
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=25596520
関連名称 25596520
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.mimet.2015.01.007
関連名称 10.1016/j.mimet.2015.01.007
権利
権利情報 © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
出版タイプ
出版タイプ NA
出版タイプResource http://purl.org/coar/version/c_be7fb7dd8ff6fe43
WoS
Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000350191500007
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