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Here, we developed a rapid assay for identifying and measuring the PML-RARA fusion gene in patients with APL using droplet-reverse transcription-polymerase chain reaction (droplet-RT-PCR) and instant quality fluorescence in situ hybridization (IQ-FISH). Methods: RNA for droplet-RT-PCR and fixed-cell suspensions for IQ-FISH were prepared from five patients with APL and three controls. We evaluated the amplification efficiency and reaction time with droplet-RT-PCR and signal clarity and hybridization time with IQ-FISH. Results: The reaction using droplet-RT-PCR was completed in 26 min. The PML-RARA fusion gene was detected in all samples from the five patients. IQ-FISH yielded clear signals after 1 h of hybridization. There were no significant differences in signal clarity or positive signal ratios between IQ-FISH and conventional FISH. Conclusions: Simultaneous droplet-RT-PCR and IQ-FISH, in addition to morphological examination of blood smears, can be used to diagnose patients as having APL within 4 h based on molecular/cytogenetic results. Rapid diagnosis can allow effective therapies to be started promptly. (C) 2015 Elsevier B.V. All rights reserved."}]}, "item_1627890986942": {"attribute_name": "\u51fa\u7248\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_be7fb7dd8ff6fe43", "subitem_version_type": "NA"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u91cd\u85e4, \u7fd4\u5e73"}], "nameIdentifiers": [{"nameIdentifier": "103720", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-06-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "13MH023A_shinsa.pdf", "filesize": [{"value": "97.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 97500.0, "url": {"label": "\u5185\u5bb9\u306e\u8981\u65e8", "url": "https://soar-ir.repo.nii.ac.jp/record/18986/files/13MH023A_shinsa.pdf"}, "version_id": "da0c89d9-e31e-470d-bca5-33ccb5dcde85"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-06-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "13MH023A_yoshi.pdf", "filesize": [{"value": "129.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 129300.00000000001, "url": {"label": "\u5be9\u67fb\u7d50\u679c\u306e\u8981\u65e8", "url": "https://soar-ir.repo.nii.ac.jp/record/18986/files/13MH023A_yoshi.pdf"}, "version_id": "bf750999-8773-4cea-b10d-905d76924c38"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-03-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "13MH023A_ronbun.pdf", "filesize": [{"value": "180.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "\u00a9 2016. 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Rapid diagnosis of acute promyelocytic leukemia with the PML-RARA fusion gene using a combination of droplet-reverse transcription-polymerase chain reaction and instant-quality fluorescence in situ hybridization(液滴型高速PCRとinstant-quality FISHを用いたPML-RARA融合遺伝子を伴う急性前骨髄性白血病の迅速診断)
http://hdl.handle.net/10091/00019748
64e10586-fddd-450c-8827-9ab821400257
名前 / ファイル | ライセンス | アクション | |
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2017-06-06 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Rapid diagnosis of acute promyelocytic leukemia with the PML-RARA fusion gene using a combination of droplet-reverse transcription-polymerase chain reaction and instant-quality fluorescence in situ hybridization(液滴型高速PCRとinstant-quality FISHを用いたPML-RARA融合遺伝子を伴う急性前骨髄性白血病の迅速診断) | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_db06 | |||||
タイプ | doctoral thesis | |||||
著者 |
重藤, 翔平
× 重藤, 翔平 |
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出版者 | ||||||
出版者 | 信州大学 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 重藤 翔平. Rapid diagnosis of acute promyelocytic leukemia with the PML-RARA fusion gene using a combination of droplet-reverse transcription-polymerase chain reaction and instant-quality fluorescence in situ hybridization(液滴型高速PCRとinstant-quality FISHを用いたPML-RARA融合遺伝子を伴う急性前骨髄性白血病の迅速診断). 信州大学, 2017, 博士論文. | |||||
書誌情報 |
発行日 2017-03-31 |
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学位授与番号 | ||||||
学位授与番号 | 13601甲第1088号 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2017-03-31 | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関名 | ||||||
学位授与機関名 | 信州大学(Shinshu university) | |||||
学位の区分 | ||||||
Doctoral | ||||||
学位の分野 | ||||||
医学 | ||||||
学位の報告番号 | ||||||
甲第1088号 | ||||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 雑誌に発表。CLINICA CHIMICA ACTA. 453:38-41 (2016); doi:10.1016/j.cca.2015.12.001. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Thesis | |||||
PubMed | ||||||
関連識別子 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=26656442 | |||||
関連名称 | ||||||
関連名称 | 26656442 | |||||
DOI | ||||||
関連識別子 | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.cca.2015.12.001 | |||||
関連名称 | ||||||
関連名称 | 10.1016/j.cca.2015.12.001 | |||||
権利 | ||||||
権利情報 | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
出版タイプ | ||||||
出版タイプ | NA | |||||
出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 | |||||
WoS | ||||||
Web of Science | ||||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000369453100005 |