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Here, we developed a rapid assay for identifying and measuring the PML-RARA fusion gene in patients with APL using droplet-reverse transcription-polymerase chain reaction (droplet-RT-PCR) and instant quality fluorescence in situ hybridization (IQ-FISH). Methods: RNA for droplet-RT-PCR and fixed-cell suspensions for IQ-FISH were prepared from five patients with APL and three controls. We evaluated the amplification efficiency and reaction time with droplet-RT-PCR and signal clarity and hybridization time with IQ-FISH. Results: The reaction using droplet-RT-PCR was completed in 26 min. The PML-RARA fusion gene was detected in all samples from the five patients. IQ-FISH yielded clear signals after 1 h of hybridization. There were no significant differences in signal clarity or positive signal ratios between IQ-FISH and conventional FISH. Conclusions: Simultaneous droplet-RT-PCR and IQ-FISH, in addition to morphological examination of blood smears, can be used to diagnose patients as having APL within 4 h based on molecular/cytogenetic results. Rapid diagnosis can allow effective therapies to be started promptly. (C) 2015 Elsevier B.V. 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  1. 050 医学部, 大学院医学系研究科
  2. 0507 学位論文
  3. 博士(医学)

Rapid diagnosis of acute promyelocytic leukemia with the PML-RARA fusion gene using a combination of droplet-reverse transcription-polymerase chain reaction and instant-quality fluorescence in situ hybridization(液滴型高速PCRとinstant-quality FISHを用いたPML-RARA融合遺伝子を伴う急性前骨髄性白血病の迅速診断)

http://hdl.handle.net/10091/00019748
64e10586-fddd-450c-8827-9ab821400257
名前 / ファイル ライセンス アクション
13MH023A_shinsa.pdf 内容の要旨 (97.5 kB)
13MH023A_yoshi.pdf 審査結果の要旨 (129.3 kB)
13MH023A_ronbun.pdf 博士論文の全文 (180.4 kB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2017-06-06
タイトル
言語 en
タイトル Rapid diagnosis of acute promyelocytic leukemia with the PML-RARA fusion gene using a combination of droplet-reverse transcription-polymerase chain reaction and instant-quality fluorescence in situ hybridization(液滴型高速PCRとinstant-quality FISHを用いたPML-RARA融合遺伝子を伴う急性前骨髄性白血病の迅速診断)
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_db06
タイプ doctoral thesis
著者 重藤, 翔平

× 重藤, 翔平

WEKO 103720

重藤, 翔平

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出版者
出版者 信州大学
引用
内容記述タイプ Other
内容記述 重藤 翔平. Rapid diagnosis of acute promyelocytic leukemia with the PML-RARA fusion gene using a combination of droplet-reverse transcription-polymerase chain reaction and instant-quality fluorescence in situ hybridization(液滴型高速PCRとinstant-quality FISHを用いたPML-RARA融合遺伝子を伴う急性前骨髄性白血病の迅速診断). 信州大学, 2017, 博士論文.
書誌情報
発行日 2017-03-31
学位授与番号
学位授与番号 13601甲第1088号
学位授与年月日
学位授与年月日 2017-03-31
学位名
学位名 博士(医学)
学位授与機関
学位授与機関名
学位授与機関名 信州大学(Shinshu university)
学位の区分
Doctoral
学位の分野
医学
学位の報告番号
甲第1088号
内容記述
内容記述タイプ Other
内容記述 雑誌に発表。CLINICA CHIMICA ACTA. 453:38-41 (2016); doi:10.1016/j.cca.2015.12.001.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Thesis
PubMed
関連識別子
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=26656442
関連名称
関連名称 26656442
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.cca.2015.12.001
関連名称
関連名称 10.1016/j.cca.2015.12.001
権利
権利情報 © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
出版タイプ
出版タイプ NA
出版タイプResource http://purl.org/coar/version/c_be7fb7dd8ff6fe43
WoS
Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000369453100005
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